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Product Description
Micro-spin column packed with media
- Significantly reduce time and expense of SPE (e.g., can top-load samples and centrifuge)
- Accomodates wide spectrum of volumes and allows precise control over rates of binding and elution
- No filters, glues, polymers, or matrices: reducing sample loss and contamination risk
TopTip enables high-fidelity chromatographic separation of even ultra-low concentration samples.
The fine 1-2 µm slit at the bottom of the TopTip permits liquid to pass through (with pressure via centrifuge or syringe), but retains chromatographicmedia in the tip. This eliminates the need for a filter – reducing dead volume, loss of sample and contamination risk.
TopTip technology has powered research resulting in many publications: Explore literature featuring TopTip.
Specifications
Sold in packs of 96, except for TT3, which are sold in packs of 20.
| Volume (µL) | Binding capacity | Media amount | |
| TT1 | 1-10 | 400 µg | 4 mg |
| TT2 | 10-200 | 1000 µg | 10 mg |
| TT3 | 100-1000 | 5000 µg | 50 mg |
Selected applications
- Antibody Enrichment TopTip
- Detergent Removal TopTip
- DNA Purification TopTip
- Glycopeptide/Glycoprotein Enrichment TopTip
- Phosphopeptide Enrichment TopTip
- Protein/Peptide Desalting TopTip
- Protein/Peptide Enrichment TopTip
Ordering information
| TopTip (1-10 ul) | TopTip (10-200 ul) | TopTip (100-1000 ul) | Gel-loader TopTip | |
| Pack of 96 | Pack of 96 | Pack of 20 | Pack of 96 | |
| Chromatographic Media | ||||
| Silica | TT1SIO / $181.00 | TT2SIO / $218.00 | TT3SIO / $118.00 | GT1SIO / $361.00 |
| C-18 | TT1C18 / $145.00 | TT2C18 / $175.00 | TT3C18 / $95.00 | GT2C18 / $289.00 |
| C-08 | TT1C08 / $145.00 | TT2C08 / $175.00 | TT3C08 / $95.00 | GT2C08 / $289.00 |
| C-04 | TT1C04 / $145.00 | TT2C04 / $175.00 | TT3C04 / $95.00 | GT2C04 / $289.00 |
| CN | TT1CN / $206.00 | TT2CN / $281.00 | TT3CN / $156.00 | GT2CN / $361.00 |
| NH2 | TT1NH2 / $206.00 | TT2NH2 / $281.00 | TT3NH2 / $156.00 | GT2NH2 / $361.00 |
| Carbon (Hypercarb) | TT1CAR / $243.00 | TT2CAR / $331.00 | TT3CAR / $206.00 | GT2CAR / $361.00 |
| C18 + Carbon (Hypercarb) | TT1MC18 / $243.00 | TT2MC18 / $331.00 | TT3MC18 / $206.00 | GT2MC18 / $361.00 |
| Cellulose | TT1CEL / $206.00 | TT2CEL / $281.00 | TT3CEL / $156.00 | GT2CEL / $361.00 |
| Zirconium dioxide | TT1ZRO / $243.00 | TT2ZRO / $331.00 | TT3ZRO / $206.00 | GT2ZRO / $361.00 |
| ZrO2+Hypercarb | TT1ZROC / $243.00 | TT2ZROC / $331.00 | TT3ZROC / $206.00 | GT2ZROC / $361.00 |
| ZrO2+Poros | TT1ZROP / $243.00 | TT2ZROP / $331.00 | TT3ZROP / $206.00 | GT2ZROP / $361.00 |
| ZrO2+Poros+Hypercarb | TT1ZROPC / $243.00 | TT2ZROPC / $331.00 | TT3ZROPC / $206.00 | GT2ZROPC / $361.00 |
| Titanium dioxide | TT1TIO / $243.00 | TT2TIO / $331.00 | TT3TIO / $206.00 | GT2TIO / $361.00 |
| TiO2+Hypercarb | TT1TIOC / $243.00 | TT2TIOC / $331.00 | TT3TIOC / $206.00 | GT2TIOC / $361.00 |
| TiO2+Poros | TT1TIOP / $243.00 | TT2TIOP / $331.00 | TT3TIOP / $206.00 | GT2TIOP / $361.00 |
| TiO2+Poros+Hypercarb | TT1TIOPC / $243.00 | TT2TIOPC / $331.00 | TT3TIOPC / $206.00 | GT2TIOPC / $361.00 |
| **NEW** ZrO2 + TiO2 | TT1TIZR / $243.00 | TT2TIZR / $331.00 | TT3TIZR / $206.00 | GT2TIZR / $361.00 |
| POROS RP-1 | TT1PR1 / $243.00 | TT2PR1 / $331.00 | TT3PR1 / $181.00 | GT2PR1 / $361.00 |
| POROS RP-2 | TT1PR2 / $243.00 | TT2PR2 / $331.00 | TT3PR2 / $181.00 | GT2PR2 / $361.00 |
| POROS weak anion exchanger | TT1PWA / $243.00 | TT2PWA / $331.00 | TT3PWA / $206.00 | GT2PWA / $361.00 |
| POROS strong anion exchanger | TT1PSA / $243.00 | TT2PSA / $331.00 | TT3PSA / $206.00 | GT2PSA / $361.00 |
| POROS strong cation exchanger | TT1PSC / $243.00 | TT2PSC / $331.00 | TT3PSC / $206.00 | GT2PSC / $361.00 |
| HILIC | TT1HIL / $206.00 | TT2HIL / $281.00 | TT3HIL / $156.00 | GT2HIL / $361.00 |
| PolyHYDROXYETHYL A | TT1PHIL / $206.00 | TT2PHIL / $281.00 | TT3PHIL / $156.00 | GT2PHIL / $361.00 |
| PolySULFOETHYL A (silica SCX) | TT1SSA / $206.00 | TT2SSA / $281.00 | TT3SSA / $156.00 | GT2SSA / $361.00 |
| PolyCAT A (silica WCX) | TT1CAT / $206.00 | TT2CAT / $281.00 | TT3CAT / $156.00 | GT2CAT / $361.00 |
| SilIca strong Anion (SAX) | TT1SAX / $206.00 | TT2SAX / $281.00 | TT3SAX / $156.00 | GT2SAX / $361.00 |
| PolyWAX LP (silica weak anion) | TT1WAX / $206.00 | TT2WAX / $281.00 | TT3WAX / $156.00 | GT2WAX / $361.00 |
| SDS-Removal | TT1SDS / $206.00 | TT2SDS / $281.00 | TT3SDS / $156.00 | GT2SDS / $361.00 |
| Affinity Media | ||||
| Silica IMAC | TT1SIMAC / $243.00 | TT2SIMAC / $331.00 | Not Available | GT2SIMAC / $361.00 |
| POROS IMAC | TT1IMAC / $243.00 | TT2IMAC / $331.00 | Not Available | GT2IMAC / $361.00 |
| Ni | TT1NIA / $243.00 | TT2NIA / $331.00 | Not Available | GT2NIA / $361.00 |
| Fe | TT1FEA / $243.00 | TT2FEA / $331.00 | Not Available | GT2FEA / $361.00 |
| Ga | TT1GAA / $243.00 | TT2GAA / $331.00 | Not Available | GT2GAA / $361.00 |
| Ca | TT1CAA / $243.00 | TT2CAA / $331.00 | Not Available | GT2CAA / $361.00 |
| Protein A | TT1PRA / $281.00 | TT2PRA / $368.00 | Not Available | GT2PRA / $361.00 |
| Protein G | TT1PRG / $318.00 | TT2PRG / $493.00 | Not Available | GT2PRG / $361.00 |
| Lectin: ConA | TT1CONA / $318.00 | TT2CONA / $443.00 | Not Available | GT2CONA / $361.00 |
| Lectin: WGA | TT1WGA / $443.00 | TT2WGA / $568.00 | Not Available | GT2WGA / $361.00 |
| Borate | TT1BOR / $306.00 | TT2BOR / $456.00 | Not Available | GT2BOR / $361.00 |
| Blue Dye | TT1BLU / $206.00 | TT2BLU / $281.00 | TT3BLU / $281.00 | GT2BLU / $361.00 |
| Red Dye | TT1RED / $206.00 | TT2RED / $281.00 | TT3RED / $281.00 | GT2RED / $361.00 |
| Immobilized Enzymes | ||||
| Trypsin | TT1TRY / $331.00 | TT2TRY / $456.00 | Not Available | GT2TRY / $361.00 |
| Trypsin +C-18 | Not Available | Not Available | Not Available | Not Available |
| Trypsin+Carbon | Not Available | Not Available | Not Available | Not Available |
| Trypsin (poroszyme) | TT1PRZ / $556.00 | TT2PRZ / $731.00 | Not Available | GT2PRZ / $361.00 |
| Sialidase | TT1SIA / $443.00 | TT2SIA / $693.00 | Not Available | Not Available |
| Trypsin adsorbed on C18 | Not Available | Not Available | Not Available | Not Available |
| Trypsin adsorbed on HILIC | Not Available | Not Available | Not Available | Not Available |
| G-10 | TT1G10 / $118.00 | TT2G10 / $118.00 | TT3G10 / $118.00 | Not Available |
| G-25 | TT1G25 / $118.00 | TT2G25 / $118.00 | TT3G25 / $118.00 | Not Available |
| G-50 | TT1G50 / $118.00 | TT2G50 / $118.00 | TT3G50 / $118.00 | Not Available |
| G-100 | TT1G100 / $118.00 | TT2G100 / $118.00 | TT3G100 / $118.00 | Not Available |
| P-2 | TT1P2 / $118.00 | TT2P2 / $118.00 | TT3P2 / $118.00 | Not Available |
| P-4 | TT1P4 / $118.00 | TT2P4 / $118.00 | TT3P4 / $118.00 | Not Available |
| P-6 | TT1P6 / $118.00 | TT2P6 / $118.00 | TT3P6 / $118.00 | Not Available |
| Empty Tip | TT1EMT / $145.00 | TT2EMT / $175.00 | TT3EMT / $95.00 | GT2EMT / $289.00 |
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