Micro-spin column (no filter) for low concentration and low volume protein/peptide samples
C18 • C08 • C04 • Carbon • HILIC • Silica/Poros SAX • Silica/Poros WAX • Silica/Poros SCX • Silica WCX

• Significantly reduce time and expense of SPE (e.g., can top-load samples and centrifuge)
• Micro-spin column format accomodates wide spectrum of volumes and allows precise control over rates of binding and elution
• No filters, glues, polymers, or matrices: reducing sample loss and contamination risk
• Ideal for enriching small sample concentrations

Protein/Peptide Enrichment TopTip micro-spin column enables separation of low-concentration/volume samples.

The fine 1-2 µm slit at the bottom of the TopTip permits liquid to pass through (via centrifuge or syringe), but retains media in the tip. This unique design eliminates the need for a filter – reducing dead volume, loss of sample and contamination risk.

Learn more about TopTip technology.

TopTip technology has powered research resulting in many publications: Explore literature featuring TopTip.

How TopTip works

Media selection
Protein/Peptide Desalting TopTips are available with a wide selection of chomatographic media:

Specifications
Sold in packs of 96, except for TT3, which are sold in packs of 20.

Selected references

• SCX: Wen-Ping Chen, et al. Cohen Microscale analysis of amino acids using gas chromatography–mass spectrometry after methyl chloroformate derivatization. Journal of Chromatography B, Vol. 878, Issue 24 (2010), p. 2199-2208.
• SSA: Scott J Walmsley, et al. Proteomic Analysis of Brush Border Membrane Vesicles Isolated from Purified Proximal Convoluted Tubules. Am J Physiol Renal Physiol (3/10/10).
• HIL: W Fremout, et al. Tryptic peptide analysis of protein binders in works of art by liquid chromatography-tandem mass spectrometry. Analytica Chimica Acta, Vol. 658, (2010), p. 156-162.

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