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Product Description

 Micro-spin column (no filter) for low concentration and low volume protein/peptide samples
C18 • C08 • C04 • Carbon • HILIC • Silica/Poros SAX • Silica/Poros WAX • Silica/Poros SCX • Silica WCX

  • Significantly reduce time and expense of SPE (e.g., can top-load samples and centrifuge)
  • Micro-spin column format accomodates wide spectrum of volumes and allows precise control over rates of binding and elution
  • No filters, glues, polymers, or matrices: reducing sample loss and contamination risk
  • Ideal for enriching small sample concentrations

Protein/Peptide Enrichment TopTip micro-spin column enables separation of low-concentration/volume samples.

The fine 1-2 µm slit at the bottom of the TopTip permits liquid to pass through (via centrifuge or syringe), but retains media in the tip. This unique design eliminates the need for a filter – reducing dead volume, loss of sample and contamination risk.

Learn more about TopTip technology.

TopTip technology has powered research resulting in many publications: Explore literature featuring TopTip.


How TopTip works

how-to-toptip.png


Media selection
Protein/Peptide Desalting TopTips are available with a wide selection of chomatographic media:

Media Use for
C18 Hydrophobic (Highest)
C08 Hydrophobic (High)
C04 Hydrophobic
Carbon (Graphite) Both hydrophobic and -philic
HILIC Hydrophilic
POROS SCX Strongly basic
Silica WCX, SCX Basic
Silica WAX, SAX Acidic
POROS WAX, SAX Strongly acidic


Specifications
Sold in packs of 96, except for TT3, which are sold in packs of 20.

  Volume (ul) Binding capacity Media amount
TT1 1-10 400 µg 4 mg
TT2 10-200 1000 µg 10 mg
TT3 100-1000 5000 µg 50 mg


Selected references

  • SCX: Wen-Ping Chen, et al. Cohen Microscale analysis of amino acids using gas chromatography–mass spectrometry after methyl chloroformate derivatization. Journal of Chromatography B, Vol. 878, Issue 24 (2010), p. 2199-2208.
  • SSA: Scott J Walmsley, et al. Proteomic Analysis of Brush Border Membrane Vesicles Isolated from Purified Proximal Convoluted Tubules. Am J Physiol Renal Physiol (3/10/10).
  • HIL: W Fremout, et al. Tryptic peptide analysis of protein binders in works of art by liquid chromatography-tandem mass spectrometry. Analytica Chimica Acta, Vol. 658, (2010), p. 156-162.


Ordering information

Part No. Price ($) Specs  
TT1C18 145 Size: TopTip (1-10 ul) Media: C-18
TT1C08 145 Size: TopTip (1-10 ul) Media: C-08
TT1C04 145 Size: TopTip (1-10 ul) Media: C-04
TT1CAR 243 Size: TopTip (1-10 ul) Media: Carbon (Graphite)
TT1HIL 206 Size: TopTip (1-10 ul) Media: HILIC
TT1PSC 243 Size: TopTip (1-10 ul) Media: POROS strong cation exchanger
TT1PWA 243 Size: TopTip (1-10 ul) Media: POROS weak anion exchanger
TT1PSA 243 Size: TopTip (1-10 ul) Media: POROS strong anion exchanger
TT1SSA 206 Size: TopTip (1-10 ul) Media: PolySULFOETHYL A (silica SCX)
TT1CAT 206 Size: TopTip (1-10 ul) Media: PolyCAT A (silica WCX)
TT1SAX 206 Size: TopTip (1-10 ul) Media: SilIca strong Anion (SAX)
TT1WAX 206 Size: TopTip (1-10 ul) Media: PolyWAX LP (silica weak anion)
TT2C18 175 Size: TopTip (10-200 ul) Media: C-18
TT2C08 175 Size: TopTip (10-200 ul) Media: C-08
TT2C04 175 Size: TopTip (10-200 ul) Media: C-04
TT2CAR 331 Size: TopTip (10-200 ul) Media: Carbon (Graphite)
TT2HIL 281 Size: TopTip (10-200 ul) Media: HILIC
TT2PSC 331 Size: TopTip (10-200 ul) Media: POROS strong cation exchanger
TT2PWA 331 Size: TopTip (10-200 ul) Media: POROS weak anion exchanger
TT2PSA 331 Size: TopTip (10-200 ul) Media: POROS strong anion exchanger
TT2SSA 281 Size: TopTip (10-200 ul) Media: PolySULFOETHYL A (silica SCX)
TT2CAT 281 Size: TopTip (10-200 ul) Media: PolyCAT A (silica WCX)
TT2SAX 281 Size: TopTip (10-200 ul) Media: SilIca strong Anion (SAX)
TT2WAX 281 Size: TopTip (10-200 ul) Media: PolyWAX LP (silica weak anion)
TT3C18 95 Size: TopTip (100-1000 ul) Media: C-18
TT3C08 95 Size: TopTip (100-1000 ul) Media: C-08
TT3C04 95 Size: TopTip (100-1000 ul) Media: C-04
TT3CAR 206 Size: TopTip (100-1000 ul) Media: Carbon (Graphite)
TT3HIL 156 Size: TopTip (100-1000 ul) Media: HILIC
TT3PSC 206 Size: TopTip (100-1000 ul) Media: POROS strong cation exchanger
TT3PWA 206 Size: TopTip (100-1000 ul) Media: POROS weak anion exchanger
TT3PSA 206 Size: TopTip (100-1000 ul) Media: POROS strong anion exchanger
TT3SSA 156 Size: TopTip (100-1000 ul) Media: PolySULFOETHYL A (silica SCX)
TT3CAT 156 Size: TopTip (100-1000 ul) Media: PolyCAT A (silica WCX)
TT3SAX 156 Size: TopTip (100-1000 ul) Media: SilIca strong Anion (SAX)
TT3WAX 156 Size: TopTip (100-1000 ul) Media: PolyWAX LP (silica weak anion)

Protein/Peptide Enrichment TopTip

Price:
$95.00


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