Micro-spin column (no filter) packed with pure desalting media for low concentration and low volume protein/peptide samples
C18 • C08 • C04 • Carbon • HILIC

• Significantly reduce time and expense of SPE (e.g., can top-load samples and centrifuge)
• Micro-spin column format accomodates wide spectrum of volumes and allows precise control over rates of binding and elution
• No filters, glues, polymers, or matrices: reducing sample loss and contamination risk
• Ideal for desalting small sample concentrations

Protein/Peptide Desalting TopTip is based on Glygen’s proprietary micro-spin column technology, enabling high-fidelity separation of low-concentration/volume samples.

The fine slit at the bottom of the TopTip (slit width: 1-2 µm) permits liquid to pass through, but retains chromatographic material in the tip. This unique design eliminates the need for a filter – reducing dead volume, loss of sample and contamination risk.

Elution pressure can be generated using any centrifuge (adaptor included), or manually with a syringe.

Learn more about TopTip technology.

TopTip technology has powered research resulting in many publications: Explore literature featuring TopTip.

How TopTip works

Media selection
Protein/Peptide Desalting TopTips are available with a wide selection of chomatographic media:

Specifications
Sold in packs of 96, except for TT3, which are sold in packs of 20.

Selected references

• C18: Hao Wang, et al. PKC-[beta]II sensitizes cardiac myofilaments to Ca2+ by phosphorylating troponin I on threonine-144. Journal of Molecular and Cellular Cardiology, Volume 41, Issue 5 (2006), p. 823-833.
• CAR: Michael Wacker, et al. Substrate specificity of bacterial oligosaccharyltransferase suggests a common transfer mechanism for the bacterial and eukaryotic systems. PNAS, Volume 103, No. 18 (2006) p. 7088-7093
• HIL: Wim Fremout, et al. Tryptic peptide analysis of protein binders in works of art by liquid chromatography-tandem mass spectrometry. Analytica Chimica Acta, Volume 658, Issue 2 (2010), p. 156-162.

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