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Product Description

 Micro-spin column (no filter) packed with pure desalting media for low concentration and low volume protein/peptide samples
C18 • C08 • C04 • Carbon • HILIC

  • Significantly reduce time and expense of SPE (e.g., can top-load samples and centrifuge)
  • Micro-spin column format accomodates wide spectrum of volumes and allows precise control over rates of binding and elution
  • No filters, glues, polymers, or matrices: reducing sample loss and contamination risk
  • Ideal for desalting small sample concentrations

Protein/Peptide Desalting TopTip is based on Glygen’s proprietary micro-spin column technology, enabling high-fidelity separation of low-concentration/volume samples.

The fine slit at the bottom of the TopTip (slit width: 1-2 µm) permits liquid to pass through, but retains chromatographic material in the tip. This unique design eliminates the need for a filter – reducing dead volume, loss of sample and contamination risk.

Elution pressure can be generated using any centrifuge (adaptor included), or manually with a syringe.

Learn more about TopTip technology.

TopTip technology has powered research resulting in many publications: Explore literature featuring TopTip.


How TopTip works

how-to-toptip.png


Media selection
Protein/Peptide Desalting TopTips are available with a wide selection of chomatographic media:

Media Use for
C18 Hydrophobic (Highest)
C08 Hydrophobic (High)
C04 Hydrophobic
Carbon (Graphite) Both hydrophobic and -philic
HILIC Hydrophilic


Specifications
Sold in packs of 96, except for TT3, which are sold in packs of 20.

  Volume (ul) Binding capacity Media amount
TT1 1-10 400 µg 4 mg
TT2 10-200 1000 µg 10 mg
TT3 100-1000 5000 µg 50 mg


Selected references

  • C18: Hao Wang, et al. PKC-[beta]II sensitizes cardiac myofilaments to Ca2+ by phosphorylating troponin I on threonine-144. Journal of Molecular and Cellular Cardiology, Volume 41, Issue 5 (2006), p. 823-833.
  • CAR: Michael Wacker, et al. Substrate specificity of bacterial oligosaccharyltransferase suggests a common transfer mechanism for the bacterial and eukaryotic systems. PNAS, Volume 103, No. 18 (2006) p. 7088-7093
  • HIL: Wim Fremout, et al. Tryptic peptide analysis of protein binders in works of art by liquid chromatography-tandem mass spectrometry. Analytica Chimica Acta, Volume 658, Issue 2 (2010), p. 156-162.


Ordering information

Part No. Price ($) Specs  
TT1C18 145 Size: TopTip (1-10 ul) Media: C-18
TT1C08 145 Size: TopTip (1-10 ul) Media: C-08
TT1C04 145 Size: TopTip (1-10 ul) Media: C-04
TT1CAR 243 Size: TopTip (1-10 ul) Media: Carbon (Graphite)
TT1HIL 206 Size: TopTip (1-10 ul) Media: HILIC
TT2C18 175 Size: TopTip (10-200 ul) Media: C-18
TT2C08 175 Size: TopTip (10-200 ul) Media: C-08
TT2C04 175 Size: TopTip (10-200 ul) Media: C-04
TT2CAR 331 Size: TopTip (10-200 ul) Media: Carbon (Graphite)
TT2HIL 281 Size: TopTip (10-200 ul) Media: HILIC
TT3C18 95 Size: TopTip (100-1000 ul) Media: C-18
TT3C08 95 Size: TopTip (100-1000 ul) Media: C-08
TT3C04 95 Size: TopTip (100-1000 ul) Media: C-04
TT3CAR 206 Size: TopTip (100-1000 ul) Media: Carbon (Graphite)
TT3HIL 156 Size: TopTip (100-1000 ul) Media: HILIC

Protein/Peptide Desalting TopTip

Price:
$95.00


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