Loading... Please wait...

Our Newsletter


Product Description

 Micro-spin column (no filter) packed with pure desalting media for low concentration and low volume protein/peptide samples
C18 • C08 • C04 • Carbon • HILIC

  • Significantly reduce time and expense of SPE (e.g., can top-load samples and centrifuge)
  • Micro-spin column format accomodates wide spectrum of volumes and allows precise control over rates of binding and elution
  • No filters, glues, polymers, or matrices: reducing sample loss and contamination risk
  • Ideal for desalting small sample concentrations

Protein/Peptide Desalting TopTip is based on Glygen’s proprietary micro-spin column technology, enabling high-fidelity separation of low-concentration/volume samples.

The fine slit at the bottom of the TopTip (slit width: 1-2 µm) permits liquid to pass through, but retains chromatographic material in the tip. This unique design eliminates the need for a filter – reducing dead volume, loss of sample and contamination risk.

Elution pressure can be generated using any centrifuge (adaptor included), or manually with a syringe.

Learn more about TopTip technology.

TopTip technology has powered research resulting in many publications: Explore literature featuring TopTip.


How TopTip works

how-to-toptip.png


Media selection
Protein/Peptide Desalting TopTips are available with a wide selection of chomatographic media:

Media Use for
C18 Hydrophobic (Highest)
C08 Hydrophobic (High)
C04 Hydrophobic
Carbon (Graphite) Both hydrophobic and -philic
HILIC Hydrophilic


Specifications
Sold in packs of 96, except for TT3, which are sold in packs of 20.

  Volume (ul) Binding capacity Media amount
TT1 1-10 400 µg 4 mg
TT2 10-200 1000 µg 10 mg
TT3 100-1000 5000 µg 50 mg


Selected references

  • C18: Hao Wang, et al. PKC-[beta]II sensitizes cardiac myofilaments to Ca2+ by phosphorylating troponin I on threonine-144. Journal of Molecular and Cellular Cardiology, Volume 41, Issue 5 (2006), p. 823-833.
  • CAR: Michael Wacker, et al. Substrate specificity of bacterial oligosaccharyltransferase suggests a common transfer mechanism for the bacterial and eukaryotic systems. PNAS, Volume 103, No. 18 (2006) p. 7088-7093
  • HIL: Wim Fremout, et al. Tryptic peptide analysis of protein binders in works of art by liquid chromatography-tandem mass spectrometry. Analytica Chimica Acta, Volume 658, Issue 2 (2010), p. 156-162.


Ordering information

Part No. Price ($) Specs  
TT1C18 145 Size: TopTip (1-10 ul) Media: C-18
TT1C08 145 Size: TopTip (1-10 ul) Media: C-08
TT1C04 145 Size: TopTip (1-10 ul) Media: C-04
TT1CAR 243 Size: TopTip (1-10 ul) Media: Carbon (Graphite)
TT1HIL 206 Size: TopTip (1-10 ul) Media: HILIC
TT2C18 175 Size: TopTip (10-200 ul) Media: C-18
TT2C08 175 Size: TopTip (10-200 ul) Media: C-08
TT2C04 175 Size: TopTip (10-200 ul) Media: C-04
TT2CAR 331 Size: TopTip (10-200 ul) Media: Carbon (Graphite)
TT2HIL 281 Size: TopTip (10-200 ul) Media: HILIC
TT3C18 95 Size: TopTip (100-1000 ul) Media: C-18
TT3C08 95 Size: TopTip (100-1000 ul) Media: C-08
TT3C04 95 Size: TopTip (100-1000 ul) Media: C-04
TT3CAR 206 Size: TopTip (100-1000 ul) Media: Carbon (Graphite)
TT3HIL 156 Size: TopTip (100-1000 ul) Media: HILIC

Protein/Peptide Desalting TopTip

Price:
$95.00


Write your own product review

Product Reviews

This product hasn't received any reviews yet. Be the first to review this product!

Customers Who Viewed This Product Also Viewed

You Recently Viewed...