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Product Description

 Micro-spin column (no filter) packed with MOAC media for low concentration phosphopeptide samples
TiO2 • ZrO2 • Mixed TiO2/ZrO2 

  • Significantly reduce time and expense of SPE (e.g., can top-load samples and centrifuge)
  • MOAC NuTip enriches phosphorylated Ser, Tyr, and Thr, in a highly sensitive and reproducible way, with minimal non-specific binding
  • Micro-spin column allows precise control over rates of binding and elution
  • No filters, glues, polymers, or matrices: reducing sample loss and contamination risk
  • Ideal for enriching small sample concentrations

Phosphopeptide Enrichment TopTip micro-spin column enables high-fidelity separation of phosphopeptides. The micro-scale of this technology ensures that phospohpeptides, often in µLtra-low concentrations, are not overwhelmed by excessive chromatographic material.

The 1-2 µm slit at the bottom of the TopTip permits liquid to pass through, but retains media. This unique design eliminates the need for a filter – reducing dead volume, loss of sample and contamination risk.

Elution pressure can be generated using any centrifuge (adaptor included), or manually with a syringe.


Learn more about TopTip technology.

TopTip technology has powered research resulting in many publications: Explore literature featuring TopTip.


How TopTip works

how-to-toptip.png


Media selection
Phosphopeptide Enrichment TopTips contain proprietary MOAC media. The literature suggests only 30% overlap in phosphopeptides isolated by TiO2 vs. ZrO2, emphasizing importance of an informed (and perhaps mixed-media) approach to enrichment.

Media Use for
TiO2 Selective for multiply phosphorylated peptides
ZrO2 Selective for monophosphorylated peptides
TiO2 / ZrO2 mixed media Broad spectrum


Specifications
Sold in packs of 96 tips. Also available in kits with a sampling of Glygen’s three MOAC media (32 tips each of TiO2, ZrO2, and TiO2+ZrO2 mixed media)

  Volume (ul) Binding capacity Media amount
TT1 1-10 400 µg 4 mg
TT2 10-200 1000 µg 10 mg
TT3 100-1000 5000 µg 50 mg


Selected references


  • Rudrabhatla, et al. ‘Quantitative phosphoproteomic analysis of neuronal intermediate filament proteins (NF-M/H) in Alzheimer’s disease by iTRAQ.’ FASEB Journal. 2010 Nov; 24(11): 4396-4407.
  • Özlü, Nurhan et al. Binding Partner Switching on Microtubules and Aurora-B in the Mitosis to Cytokinesis Transition. Mol Cell Proteomics, 2010 9: 336-350.


Ordering information

Part No. Price ($) Specs  
TT1TIO 243 Size: TopTip (1-10 µL) Media: Titanium dioxide
TT1ZRO 243 Size: TopTip (1-10 µL) Media: Zirconium dioxide
TT1TIZR 243 Size: TopTip (1-10 µL) Media: TiO2+ZrO2
TT2TIO 331 Size: TopTip (10-200 µL) Media: Titanium dioxide
TT2ZRO 331 Size: TopTip (10-200 µL) Media: Zirconium dioxide
TT2TIZR 331 Size: TopTip (10-200 µL) Media: TiO2+ZrO2
TT3TIO 206 Size: TopTip (100-1000 µL) Media: Titanium dioxide
TT3ZRO 206 Size: TopTip (100-1000 µL) Media: Zirconium dioxide
TT3TIZR 206 Size: TopTip (100-1000 µL) Media: TiO2+ZrO2
TT1KITPHOS 269 Size: TopTip (1-10 µL) Media: Selection of TiO2, ZrO2, and mixed TiO2+ZrO2
TT2KITPHOS 355 Size: TopTip (10-200 µL) Media: Selection of TiO2, ZrO2, and mixed TiO2+ZrO2

Phosphopeptide Enrichment TopTip

Price:
$206.00


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