Loading... Please wait...

Our Newsletter


Product Description

 Micro-spin column (no filter) packed with MOAC media for low concentration phosphopeptide samples
TiO2 • ZrO2 • Mixed TiO2/ZrO2 

  • Significantly reduce time and expense of SPE (e.g., can top-load samples and centrifuge)
  • MOAC NuTip enriches phosphorylated Ser, Tyr, and Thr, in a highly sensitive and reproducible way, with minimal non-specific binding
  • Micro-spin column allows precise control over rates of binding and elution
  • No filters, glues, polymers, or matrices: reducing sample loss and contamination risk
  • Ideal for enriching small sample concentrations

Phosphopeptide Enrichment TopTip micro-spin column enables high-fidelity separation of phosphopeptides. The micro-scale of this technology ensures that phospohpeptides, often in µLtra-low concentrations, are not overwhelmed by excessive chromatographic material.

The 1-2 µm slit at the bottom of the TopTip permits liquid to pass through, but retains media. This unique design eliminates the need for a filter – reducing dead volume, loss of sample and contamination risk.

Elution pressure can be generated using any centrifuge (adaptor included), or manually with a syringe.


Learn more about TopTip technology.

TopTip technology has powered research resulting in many publications: Explore literature featuring TopTip.


How TopTip works

how-to-toptip.png


Media selection
Phosphopeptide Enrichment TopTips contain proprietary MOAC media. The literature suggests only 30% overlap in phosphopeptides isolated by TiO2 vs. ZrO2, emphasizing importance of an informed (and perhaps mixed-media) approach to enrichment.

Media Use for
TiO2 Selective for multiply phosphorylated peptides
ZrO2 Selective for monophosphorylated peptides
TiO2 / ZrO2 mixed media Broad spectrum


Specifications
Sold in packs of 96 tips. Also available in kits with a sampling of Glygen’s three MOAC media (32 tips each of TiO2, ZrO2, and TiO2+ZrO2 mixed media)

  Volume (ul) Binding capacity Media amount
TT1 1-10 400 µg 4 mg
TT2 10-200 1000 µg 10 mg
TT3 100-1000 5000 µg 50 mg


Selected references


  • Rudrabhatla, et al. ‘Quantitative phosphoproteomic analysis of neuronal intermediate filament proteins (NF-M/H) in Alzheimer’s disease by iTRAQ.’ FASEB Journal. 2010 Nov; 24(11): 4396-4407.
  • Özlü, Nurhan et al. Binding Partner Switching on Microtubules and Aurora-B in the Mitosis to Cytokinesis Transition. Mol Cell Proteomics, 2010 9: 336-350.


Ordering information

Part No. Price ($) Specs  
TT1TIO 243 Size: TopTip (1-10 µL) Media: Titanium dioxide
TT1ZRO 243 Size: TopTip (1-10 µL) Media: Zirconium dioxide
TT1TIZR 243 Size: TopTip (1-10 µL) Media: TiO2+ZrO2
TT2TIO 331 Size: TopTip (10-200 µL) Media: Titanium dioxide
TT2ZRO 331 Size: TopTip (10-200 µL) Media: Zirconium dioxide
TT2TIZR 331 Size: TopTip (10-200 µL) Media: TiO2+ZrO2
TT3TIO 206 Size: TopTip (100-1000 µL) Media: Titanium dioxide
TT3ZRO 206 Size: TopTip (100-1000 µL) Media: Zirconium dioxide
TT3TIZR 206 Size: TopTip (100-1000 µL) Media: TiO2+ZrO2
TT1KITPHOS 269 Size: TopTip (1-10 µL) Media: Selection of TiO2, ZrO2, and mixed TiO2+ZrO2
TT2KITPHOS 355 Size: TopTip (10-200 µL) Media: Selection of TiO2, ZrO2, and mixed TiO2+ZrO2

Phosphopeptide Enrichment TopTip

Price:
$206.00


Write your own product review

Product Reviews

This product hasn't received any reviews yet. Be the first to review this product!

Customers Who Viewed This Product Also Viewed

You Recently Viewed...