Micro-spin column (no filter) packed with MOAC media for low concentration phosphopeptide samples
TiO2 • ZrO2 • Mixed TiO2/ZrO2 

• Significantly reduce time and expense of SPE (e.g., can top-load samples and centrifuge)
• MOAC NuTip enriches phosphorylated Ser, Tyr, and Thr, in a highly sensitive and reproducible way, with minimal non-specific binding
• Micro-spin column allows precise control over rates of binding and elution
• No filters, glues, polymers, or matrices: reducing sample loss and contamination risk
• Ideal for enriching small sample concentrations

Phosphopeptide Enrichment TopTip micro-spin column enables high-fidelity separation of phosphopeptides. The micro-scale of this technology ensures that phospohpeptides, often in µLtra-low concentrations, are not overwhelmed by excessive chromatographic material.

The 1-2 µm slit at the bottom of the TopTip permits liquid to pass through, but retains media. This unique design eliminates the need for a filter – reducing dead volume, loss of sample and contamination risk.

Elution pressure can be generated using any centrifuge (adaptor included), or manually with a syringe.

Learn more about TopTip technology.

TopTip technology has powered research resulting in many publications: Explore literature featuring TopTip.

How TopTip works

Media selection
Phosphopeptide Enrichment TopTips contain proprietary MOAC media. The literature suggests only 30% overlap in phosphopeptides isolated by TiO2 vs. ZrO2, emphasizing importance of an informed (and perhaps mixed-media) approach to enrichment.

Specifications
Sold in packs of 96 tips. Also available in kits with a sampling of Glygen’s three MOAC media (32 tips each of TiO2, ZrO2, and TiO2+ZrO2 mixed media)

Selected references

• Rudrabhatla, et al. ‘Quantitative phosphoproteomic analysis of neuronal intermediate filament proteins (NF-M/H) in Alzheimer’s disease by iTRAQ.’ FASEB Journal. 2010 Nov; 24(11): 4396-4407.
• Özlü, Nurhan et al. Binding Partner Switching on Microtubules and Aurora-B in the Mitosis to Cytokinesis Transition. Mol Cell Proteomics, 2010 9: 336-350.

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