Loading... Please wait...

Our Newsletter


Product Description

 Micro-spin column (no filter) for low concentration and low volume glycopeptide/-protein samples
ConA lectin • WGA lectin • Silica/Poros SAX• Carbon

  • Significantly reduce time and expense of SPE (e.g., can top-load samples and centrifuge)
  • Immobilized lectin TopTip enriches glycosylated peptides/proteins, in a highly sensitive and reproducible way, with minimal non-specific binding
  • Micro-spin column allows precise control over rates of binding and elution
  • No filters, glues, polymers, or matrices: reducing sample loss and contamination risk
  • Ideal for enriching small sample concentrations

Glycopeptide/Glycoprotein Enrichment TopTip micro-spin column, allows selective recovery of glycosylated peptides/proteins known to have important biological function in immune regulation, inflammation, cell-to-cell adhesion, and cell signaling.

The fine slit at the bottom of the TopTip (slit width: 1-2 µm) permits liquid to pass through, but retains media in the tip. This unique design eliminates the need for a filter – reducing dead volume, loss of sample and contamination risk.

Elution pressure can be generated using any centrifuge (adaptor included), or manually with a syringe.

Learn more about TopTip technology.

TopTip technology has powered research resulting in many publications: Explore literature featuring TopTip.


How TopTip works

how-to-toptip.png


Media selection
Protein/Peptide Enrichment TopTips are available with a wide selection of chomatographic media:

Media Use for
ConA lectin Alpha-linked mannose and terminal glucose residues
WGA lectin N-Acetyl glucosamine (GlcNAc) groups and sialic acid
SAX Sulfated glycan
Carbon (Graphite) Both hydrophobic and -philic


Specifications
Sold in packs of 96 tips. Also available in kits with a sampling of Glygen’s three MOAC media (32 tips each of TiO2, ZrO2, and TiO2+ZrO2 mixed media)

  Volume (ul) Binding capacity Media amount
TT1 1-10 400 µg 4 mg
TT2 10-200 1000 µg 10 mg
TT3 100-1000 5000 µg 50 mg


Selected references

  • Lectins: N. I. Taranenko, et al.  Effects of Lectins Affinity Chromatography on Glycoproteins Enrichment Using AP-MALDI Ion Trap Mass Spectrometry. Proceedings of 52nd ASMS Conference on Mass Spectrometry and Allied Topics (2004).
  • SAX: Ming Lei, et al. Sequential Enrichment of Sulfated Glycans by Strong Anion-Exchange Chromatography Prior to Mass Spectrometric Measurements. J Am Soc Mass Spectrom, 21(2010), p. 348-357.


Ordering information

Part No. Price ($) Specs  
TT1CONA 318 Size: TopTip (1-10 ul) Media: Lectin: ConA
TT1WGA 443 Size: TopTip (1-10 ul) Media: Lectin: WGA
TT1SAX 206 Size: TopTip (1-10 ul) Media: SilIca strong Anion (SAX)
TT1PSA 243 Size: TopTip (1-10 ul) Media: POROS strong anion exchanger
TT1CAR 243 Size: TopTip (1-10 ul) Media: Carbon (Graphite)
TT2CONA 443 Size: TopTip (10-200 ul) Media: Lectin: ConA
TT2WGA 568 Size: TopTip (10-200 ul) Media: Lectin: WGA
TT2SAX 281 Size: TopTip (10-200 ul) Media: SilIca strong Anion (SAX)
TT2PSA 331 Size: TopTip (10-200 ul) Media: POROS strong anion exchanger
TT2CAR 331 Size: TopTip (10-200 ul) Media: Carbon (Graphite)
TT3CONA 318 Size: TopTip (100-1000 ul) Media: Lectin: ConA
TT3WGA 443 Size: TopTip (100-1000 ul) Media: Lectin: WGA
TT3SAX 206 Size: TopTip (100-1000 ul) Media: SilIca strong Anion (SAX)
TT3PSA 243 Size: TopTip (100-1000 ul) Media: POROS strong anion exchanger
TT3CAR 243 Size: TopTip (100-1000 ul) Media: Carbon (Graphite)

Glycopeptide/Glycoprotein Enrichment TopTip

Price:
$206.00


Write your own product review

Product Reviews

This product hasn't received any reviews yet. Be the first to review this product!

Customers Who Viewed This Product Also Viewed

You Recently Viewed...