Micro-spin column (no filter) for low concentration and low volume glycopeptide/-protein samples
ConA lectin • WGA lectin • Silica/Poros SAX• Carbon

• Significantly reduce time and expense of SPE (e.g., can top-load samples and centrifuge)
• Immobilized lectin TopTip enriches glycosylated peptides/proteins, in a highly sensitive and reproducible way, with minimal non-specific binding
• Micro-spin column allows precise control over rates of binding and elution
• No filters, glues, polymers, or matrices: reducing sample loss and contamination risk
• Ideal for enriching small sample concentrations

Glycopeptide/Glycoprotein Enrichment TopTip micro-spin column, allows selective recovery of glycosylated peptides/proteins known to have important biological function in immune regulation, inflammation, cell-to-cell adhesion, and cell signaling.

The fine slit at the bottom of the TopTip (slit width: 1-2 µm) permits liquid to pass through, but retains media in the tip. This unique design eliminates the need for a filter – reducing dead volume, loss of sample and contamination risk.

Elution pressure can be generated using any centrifuge (adaptor included), or manually with a syringe.

Learn more about TopTip technology.

TopTip technology has powered research resulting in many publications: Explore literature featuring TopTip.

How TopTip works

Media selection
Protein/Peptide Enrichment TopTips are available with a wide selection of chomatographic media:

Specifications
Sold in packs of 96 tips. Also available in kits with a sampling of Glygen’s three MOAC media (32 tips each of TiO2, ZrO2, and TiO2+ZrO2 mixed media)

Selected references

• Lectins: N. I. Taranenko, et al.  Effects of Lectins Affinity Chromatography on Glycoproteins Enrichment Using AP-MALDI Ion Trap Mass Spectrometry. Proceedings of 52nd ASMS Conference on Mass Spectrometry and Allied Topics (2004).
• SAX: Ming Lei, et al. Sequential Enrichment of Sulfated Glycans by Strong Anion-Exchange Chromatography Prior to Mass Spectrometric Measurements. J Am Soc Mass Spectrom, 21(2010), p. 348-357.

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