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Scientists from all over the world trust Glygen products for their most important experiments.  Below is a list of the newest publications that have successfully used Glygen. 

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MOST RECENT PUBLICATIONS 

 

 

Application Glygen product Media Year Publication Excerpt
Peptides Filter Plate 5-7ul C18 2013 Erik Portelius, Maria Olsson, Gunnar Brinkmalm, Ulla Rüetschi, Niklas Mattsson, Ulf Andreasson, Johan Gobom, Ann Brinkmalm, Mikko Hölttä, Kaj Blennow, Henrik Zetterberg
Mass Spectrometric Characterization of Amyloid-β Species in the 7PA2 Cell Model of Alzheimer's Disease
Journal of Alzheimer's Disease, Volume 33, Number 1 / 2013, 85-93
 
Small molecules NuTip TiO2 2012 Hyun Ju Yoo and Kristina Hâkansson
Phosphate-containing Metabolite Enrichment with TiO2 Micro-tips
Bull. Korean Chem. Soc. 2012, Vol. 33, No. 8 1
"Samples were desalted prior to mass spectrometric analyses using NuTipTM graphitized carbon 10μl pipette tips (Glygen Corp., Columbia, MD). Tips were conditioned by pipetting five times 10μl of 60% acetonitrile containing 0.05% trifluoroacetic acid (TFA) followed by three times 10μl of water."
      2012 Hansuk Buncherda, Merel A. Nessena, Niels Nousea, Sacha K. Steldera, Winfried Rosebooma, Henk L. Dekkera, Jos C. Arentsb, Linde E. Smeenkc, Martin J. Wannerc, Jan H. van Maarseveenc, Xiao Yangd, Peter J. Lewisd, Leo J. de Koninga, Chris G. de Kostera, Luitzen de Jonga
Selective enrichment and identification of cross-linked peptides to study 3-D structures of protein complexes by mass spectrometry
Journal of Proteomics, Volume 75, Issue 7, 3 April 2012, Pages 2205–2215
TT3C18
"Following reduction all samples were desalted using porous graphitized carbon Top Tip solid phase extraction cartridges (Glygen, Columbia, MD) and the human milk oligosaccharides (HMOs) were eluted..."
    TiO2 2012 Iris L. Batalha, Christopher R. Lowe2, Ana C.A. Roque
Platforms for enrichment of phosphorylated proteins and peptides in proteomics
Volume 30, Issue 2, February 2012, Pages 100–110
"Sequential enrichment of sulfated glycans is attained using spin-columns packed with strong anion exchanger"
  NuTip C8 and Carbon 2012 Mariana Barboza, Janneth Pinzon, Saumya Wickramasinghe, John W. Froehlich, Isabelle Moeller, Jennifer T. Smilowitz, L. Renee Ruhaak, Jincui Huang, Bo Lönnerdal‖, J. Bruce German, Juan F. Medrano, Bart C. Weimer and Carlito B. Lebrilla.
Glycosylation of Human Milk Lactoferrin Exhibits Dynamic Changes During Early Lactation Enhancing Its Role in Pathogenic Bacteria-Host Interactions.
June 1, 2012 Molecular & Cellular Proteomics, 11, M111.015248.
 
  Nutip C18 and C4 2012 C. Fernández-Costaa, C. Ruiz-Romeroa, F.J. Blancoa, H.M. Santosb, J.L. Capelo
An assessment of the indirect high intensityultrasonic assisted cleavage of complex proteomeswith immobilized trypsin.
Talanta Available online 22 December 2012
 
    TiO2 2012 Wolfgang Hoehenwarter, Martin Thomas, Ella Nukarinen, Volker Egelhofer, Horst Roehrig, Wolfram Weckwerth, Uwe Conrath and Gerold J. M. Beckers.
Tandem metal oxide affinity chromatography identifies novel in vivo MAP kinase substrates in Arabidopsis thaliana. MCP Papers in Press. Published on November 20, 2012 as Manuscript M112.020560
"Micropipette tips (TopTip 10–200 μl) filled with protein A affin- ity medium were obtained from Glygen Corp."
  TopTip C18 2012 Francesco Bonardi, Fabrizia Fusetti, Patrick Deelen, Djoke van Gosliga, Edo, Vellenga and Jan Jacob Schuringa.
A Proteomics and transcriptomics approach to identify leukemic stem cell Markers
MCP Papers in Press. Published on December 11, 2012 as Manuscript M112.021931
"[SDS removal protocol using] TopTip filters with Poly- Hydroxyethyl (HILIC) resin..."
    TiO2 2012 François Griaud, Andrew J.K. Williamson, Samuel Taylor, David N. Potier, Elaine Spooncer, Andrew Pierce, Anthony D. Whetton
BCR/ABL modulates protein phosphorylation associated with the etoposide-induced DNA damage response
Journal of Proteomics, Volume 77, 21 December 2012, Pages 14–26
 
  NuTip C18 2012 Jesús Mateosa, Lucía Louridoa, Patricia Fernández-Puentea, Valentina Calamiaa, Carlos Fernández-Lópezb, Natividad Oreirob, Cristina Ruiz-Romeroa, Francisco J. Blancoa
Differential protein profiling of synovial fluid from rheumatoid arthritis and osteoarthritis patients using LC–MALDI TOF/TOF; Journal of Proteomics, Volume 75, Issue 10, 6 June 2012, Pages 2869–2878
 
    TiO2 2012 Jean Charles Isner, Thomas Nu hse and Frans J. M. Maathuis,
The cyclic nucleotide cGMP is involved in plant hormone signalling and alters phosphorylation of Arabidopsis thaliana root proteins.
Journal of Experimental Botany, Vol. 63, No. 8, pp. 3199–3205, 2012
 
  NuTip Carbon 2012 Takashi Nishikaze , Yuko Fukuyama , Shin-ichirou Kawabata , and Koichi Tanaka
Sensitive Analyses of Neutral N-Glycans using Anion-Doped Liquid Matrix G3CA by Negative-Ion Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry
Anal. Chem., 2012, 84 (14), pp 6097–6103
NT2CAR
"Sample treatment can also be partly performed using an integrated 'MALDI pen' (Glygen, 2009)"
  NuTip C18 2012 Mohammad SAADATI BEZDI, Reza FARSHBAF POURABAD, Mahmoud TOORCHI, Nosratollah ZARGHAMI, Setsuko KOMATSU.
Protein patterns in the salivary gland of the sunn pest, Eurygaster integriceps (Put.) (Hemiptera: Scutelleridae)
Türk. entomol. derg., 2012, 36 (2): 215-223
 
  TopTip Empty 2012 Wei Jiang, Oksana Lockridge
Detectable organophosphorus pesticide exposure in the blood of Nebraska and Iowa residents measured by mass spectrometry of butyrylcholinesterase adducts
Chemico-Biological Interactions: Available online 16 September 2012
 
  TopTip C4 2012 Igor V. Peshenko, Elena V. Olshevskaya, Sunghyuk Lim, James B. Ames and Alexander M. Dizhoor
Calcium-Myristoyl Tug Is a New Mechanism for Intramolecular Tuning of Calcium Sensitivity and Target Enzyme Interaction for Guanylyl Cyclase-activating Protein :DYNAMIC CONNECTION BETWEEN N-FATTY ACYL GROUP AND EF-HAND CONTROLS CALCIUM SENSITIVITY
J. Biol. Chem. 2012 287: 13972-13984.
 
  NuTip C18 2012 Carolina Fernández-Costa, Valentina Calamia, Patricia Fernández-Puente, José-Luis Capelo-Martínez,
Cristina Ruiz-Romero, and Francisco J Blanco
Sequential depletion of human serum for the search of osteoarthritis biomarkers
Fernández-Costa et al. Proteome Science 2012, 10:55
 
  NuTip Carbon 2012 Kaoru Kaneshiro, Makoto Watanabe, Kazuya Terasawa, Hiromasa Uchimura, Yuko Fukuyama, Shinichi Iwamoto , Taka-Aki Sato , Kazuharu Shimizu, Gozoh Tsujimoto,, and Koichi Tanaka
Rapid Quantitative Profiling of N-Glycan by the Glycan-Labeling Method Using 3-Aminoquinoline/α-Cyano-4-hydroxycinnamic Acid
Anal. Chem., 2012, 84 (16), pp 7146–7151
 
  NuTip Carbon 2012 Hannu Peltoniemi, Suvi Natune, Ilja Ritamo, Leena Valmu, Jarkko Räbinä
Novel data analysis tool for semiquantitative LC-MS-MS2 profiling of N-glycans
Glycoconjugate Journal June 2012
 
  NuTip C18 2012 Salavati, Afshin; Taleei, Alireza; Akbar Shahnejat Bushehri, Ali; Komatsu, Setsuko
Analysis of the Proteome of Common Bean (Phaseolus vulgaris L.) Roots after Inoculation with Rhizobium etli Protein and Peptide Letters, Volume 19, Number 8
The peptide solution ob- tained was dried, re-concentrated with 30 μL of 0.1% trifluoroacetic acid
and desalted with NuTip C-18 pipet tips (Glygen, Columbia, MD, USA
  TopTip C18 2012 Dana M Freund, Jessica E. Prenni, and Norman P. Curthoys
Response of the Mitochondrial Proteome of Rat Renal Proximal Convoluted Tubules to Chronic Metabolic Acidosis Am J Physiol Renal Physiol ajprenal.00526.2012; published ahead of print November 7, 2012,
 
    Carbon 2012 Lebrilla and David A. Mills, Kemin Tan, Hyun-Ju Eom, Andrzej Joachimiak, Carlito B. David A. Sela, Daniel Garrido, Larry Lerno, Shuai Wu
Fucosylated Human Milk Oligosaccharides
ATCC 15697 a-Fucosidases Are Active on
Bifidobacterium longum subsp. Infantis 2 December 2011.10.1128/AEM.06762-11.
Appl. Environ. Microbiol. 2012, 78(3):795. DOI:
 
      2012 Valentina Calamia, Lucía Lourido, Patricia Fernández-Puente, Jesús Mateos, Beatriz Rocha, Eulalia Montell, Josep Vergés, Cristina Ruiz-Romero, and Francisco J Blanco
Secretome analysis of chondroitin sulfate-treated chondrocytes reveals anti-angiogenic, anti-inflammatory and anti-catabolic properties
Arthritis Research & Therapy 2012, 14:R202
 
    C18 2012 Mohammad Saadati Bezdi, Mahmoud Toorchi, Reza Farshbaf Pourabad, Nosratollah Zarghami, Mohammad-Zaman Nouri, Setsuko Komatsu
PROTEOME ANALYSIS OF GUT AND SALIVARY GLAND PROTEINS OF FIFTH-INSTAR NYMPH AND ADULTS OF THE SUNN PEST, Eurygaster integriceps
Archives of Insect Biochemistry and Physiology: Volume 81, Issue 2, pages 105–119, October 2012
 
    Carbon 2012 Tia Hirvonen, Heli Suila, Annika Kotovuori, Ilja Ritamo, Annamari Heiskanen, Pertti Sistonen, Heidi Anderson, Tero Satomaa, Juhani Saarinen, Sari Tiitinen, Jarkko Räbinä, Saara Laitinen, Suvi Natunen, and Leena Valmu
The i Blood Group Antigen as a Marker for Umbilical Cord Blood-Derived Mesenchymal Stem Cells
Stem Cells and Development. February 10, 2012, 21(3): 455-464. doi:10.1089/scd.2011.0405.
 
    C18 2012 Hironori Kogaa,   Koji Dohib, Takumi Nishiuchic, Tomoaki Katoc, Hiroyuki Takaharaa, Masashi Morid, Setsuko Komatsue
Proteomic analysis of susceptible rice plants expressing the whole plant-specific resistance against Magnaporthe oryzae: Involvement of a thaumatin-like protein
Physiological and Molecular Plant Pathology: Volume 77, Issue 1, January 2012, Pages 60–66
 
    TiO2 2012 Yanmei Chen, Peng Liu , Wolfgang Hoehenwarter , and Jinxing Lin
Proteomic and phosphoproteomic analysis of Picea wilsonii pollen development under nutrient limitation.
J. Proteome Res., 2012, 11 (8), pp 4180–4190
 
  NuTip C18 2012 Nagib Ahsan, Takuji Nakamura, Setsuko Komatsu
Differential responses of microsomal proteins and metabolites in two contrasting cadmium (Cd)-accumulating soybean cultivars under Cd stress
Amino Acids (2012) 42:317–327
 
    C18 2012 Yohei Nanjo, Ludovit Skultety , L'ubica Uváčková , Katarína Klubicová , Martin Hajduch , and Setsuko Komatsu
Mass Spectrometry-Based Analysis of Proteomic Changes in the Root Tips of Flooded Soybean Seedlings
J. Proteome Res., 2012, 11 (1), pp 372–385
 
    Carbon 2012 Zi-Wei Chen, Li-Hai Chen, Natalia Akentieva, Cheryl F. Lichti, Ramin Darbandi, Randy Hastings, Douglas F. Covey, David E. Reichert, R. Reid Townsend, Alex S. Evers
A neurosteroid analogue photolabeling reagent labels the colchicine-binding site on tubulin: A mass spectrometric analysis
ELECTROPHORESIS Volume 33, Issue 4, Article first published online: 27 MAR 2012
 
  NuTip Carbon 2012 Céline Marionneau, Cheryl F. Lichti, Pierre Lindenbaum, Flavien Charpentier, Jeanne M Nerbonne , R. Reid Townsend, and Jean Mérot
Mass Spectrometry-Based Identification of Native Cardiac Nav1.5 Channel α Subunit Phosphorylation Sites
J. Proteome Res., 2012, 11 (12), pp 5994–6007
 
  TopTip C18 2012 Rikard Fristedt, Wioleta Wasilewska, Elzbieta Romanowska, Alexander V. Vener
Differential phosphorylation of thylakoid proteins in mesophyll and bundle sheath chloroplasts from maize plants grown under low or high light
PROTEOMICS Volume 12, Issue 18, pages 2852–2861, September 2012
 
  NuTip C18 2012 Zahed Hossaina, Takahiro Makinoc, Setsuko Komatsua
Proteomic study of β-aminobutyric acid-mediated cadmium stress alleviation in soybean Journal of Proteomics
Volume 75, Issue 13, 16 July 2012, Pages 4151–4164
 
  NuTip C18 2012 Zahed Hossain, Makita Hajika, Setsuko Komatsu
Comparative proteome analysis of high and low cadmium accumulating soybeans under cadmium stress
Amino Acids (2012) 43:2393–2416
 
      2012 Lindenburg, Petrus Wilhelmus
New electromigration-driven enrichment techniques for peptidomics and metabolomics
Lindenburg, P.W., 2012, Doctoral thesis, Leiden University
 
  TwistTip SCX 2012 Will I. Menzel, Wen-Ping Chen, Adrian D. Hegeman, Jerry D. Cohen
Qualitative and Quantitative Screening of Amino Acids in Plant Tissues High-Throughput Phenotyping in Plants Methods in Molecular Biology Volume 918, 2012, pp 165-178
 
  NuTip C18 2012 Afshin Salavatia, b, Ali Akbar Shahnejat Bushehrib, Alireza Taleeib, Susumu Hiragaa, Setsuko Komatsua
A comparative proteomic analysis of the early response to compatible symbiotic bacteria in the roots of a supernodulating soybean variety
Journal of Proteomics Volume 75, Issue 3, 4 January 2012, Pages 819–832
 
  NuTip C18 2012 Afshin Salavatia, b, 1, Amana Khatoona, c, 1, Yohei Nanjoa, 1, Setsuko Komatsua,
Analysis of proteomic changes in roots of soybean seedlings during recovery after flooding
Journal of Proteomics Volume 75, Issue 3, 4 January 2012, Pages 878–893
 
  NuTip Bravo Automation carbon 2012 Andrew R. Lee, Rachel R. Lamb, Julietta H. Chang, Petra Erdmann-Gilmore, Cheryl F. Lichti , Henry W. Rohrs , James P. Malone, Yogesh P. Wairkar , Aaron DiAntonio, R. Reid Townsend, and Susan M. Culican
Identification of Potential Mediators of Retinotopic Mapping: A Comparative Proteomic Analysis of Optic Nerve from WT and Phr1 Retinal Knockout Mice
J. Proteome Res., 2012, 11 (11), pp 5515–5526
 
  NuTip Bravo Automation Carbon 2012 Zi-Wei Chen, Brad Manion, R. Reid Townsend, David E. Reichert, Douglas F. Covey, Joe Henry Steinbach, Werner Sieghart, Karoline Fuchs and Alex S. Evers
Neurosteroid Analog Photolabeling of a Site in the Third Transmembrane Domain of the β3 Subunit of the GABAA Receptor
Molecular Pharmacology September 2012 vol. 82 no. 3 408-419
Bravo carbon
 
  NuTip Bravo Automation Carbon 2012 Zi-Wei Chen, Karoline Fuchs, Werner Sieghart, R. Reid Townsend, and Alex S. Evers
Deep Amino Acid Sequencing of Native Brain GABAA Receptors Using High-Resolution Mass Spectrometry
Mol Cell Proteomics 2012 11: M111.011445. First Published on October 3, 2011, doi:10.1074/mcp.M111.011445
 
  NuTip C18 2012 Hiroko Sawadaa, Setsuko Komatsub, Yohei Nanjob, Nisar Ahmad Khana, Yoshihisa Kohnoa
Proteomic analysis of rice response involved in reduction of grain yield under elevated ozone stress
Environmental and Experimental Botany Volume 77, April 2012, Pages 108–116
 
  NuTip C18 2012 Valentina Calamia, Patricia Fernández-Puente, Jesús Mateos, Lucía Lourido, Beatriz Rocha, Eulália Montell, Josep Vergés, Cristina Ruiz-Romero, and Francisco J. Blanco
Pharmacoproteomic Study of Three Different Chondroitin Sulfate Compounds on Intracellular and Extracellular Human Chondrocyte Proteomes
Mol Cell Proteomics 2012 11: M111.013417
 
  NuTip C18 2012 Beatriz Rocha, Valentina Calamia, Jesús Mateos, Patricia Fernández-Puente, Francisco J. Blanco, and Cristina Ruiz-Romero
Metabolic Labeling of Human Bone Marrow Mesenchymal Stem Cells for the Quantitative Analysis of their Chondrogenic Differentiation
J. Proteome Res., 2012, 11 (11), pp 5350–5361
 
  NuTip   2012 Jose Antonio Mendez, Nelson C. Soares, Jesús Mateos, Carmen Gayoso, Carlos Rumbo, Jesús Aranda, Maria Tomas, and Germán Bou
Extracellular Proteome of a Highly Invasive Multidrug-resistant Clinical Strain of Acinetobacter baumannii
J. Proteome Res., 2012, 11 (12), pp 5678–5694
NuTip
 
      2012 F Dycka - is.muni.cz
Identification and characterization of proteins regulated by plant hormones cytokinins by mass spectrometry
Thesis Brno, 2012
 
      2012 Wim Fremouta, Maarten Dhaenensc, Steven Saverwynsa, Jana Sanyovaa, Peter Vandenabeeled, Dieter Deforcec, Luc Moens
Development of a dedicated peptide tandem mass spectral library for conservation science
Analytica Chimica Acta Volume 728, 30 May 2012, Pages 39–48
 
      2012 Federico Ferro , X Renza Spelat, X Elisa Puppato, X Antonio Paolo Beltrami, X Daniela Cesselli, X Giuseppe Falini, Carlo Alberto Beltrami, Ferro F, Spelat R, D'Aurizio F, Puppato E, Pandolfi M, et al.
Dental Pulp Stem Cells Differentiation Reveals New Insights in Oct4A Dynamics. PLoS ONE 7(7): e41774. doi:10.1371/journal.pone.0041774(2012)
 
      2012 Nithya Krishnana, TuKiet T. Lamc, Andrew Fritzd, Donald Rempinskia, Kieran O'Loughline, Hans Mindermane, Ronald Berezneyd, William F. Marzlufff and Roopa Thapara,
The Prolyl Isomerase Pin1 Targets Stem-Loop Binding Protein (SLBP) To Dissociate the SLBP-Histone mRNA Complex Linking Histone mRNA Decay with SLBP Ubiquitination
Mol. Cell. Biol. November 2012 vol. 32 no. 21 4306-4322
 
      2012 Nithya Krishnan1, TuKiet T. Lam, Andrew Fritz, Donald Rempinski, Kieran O’ Loughlin, Hans Minderman, Ronald Berezney, William F. Marzluff and Roopa Thapar
THE PROLYL ISOMERASE PIN1 TARGETS SLBP TO DISSOCIATE THE SLBP-HISTONE mRNA COMPLEX LINKING HISTONE mRNA DECAY WITH SLBP UBIQUITINATION
Published ahead of print 20 August 2012, doi: 10.1128/MCB.00382-12 ,MCB.00382-12
 
    Carbon 2012 Pia H Jensen, Niclas G Karlsson, Daniel Kolarich,& Nicolle H Packer
Determination of site-specific glycan heterogeneity on glycoproteins
Nature Protocols Volume:7,Pages:1299–1310Year published:(2012)DOI:doi:10.1038/nprot.2012.063
 
  TopTip SCX 2012 Xenia Gatsos, David L Steer, Thamradeen A Junaid, A Ian Smith, Ben Adler, M John Albert
Application of protein purification methods for the enrichment of a cytotoxin from Campylobacter jejuni
BMC Microbiology 2012, 12:303 doi:10.1186/1471-2180-12-303
 
      2012 Mohammad-Zaman Nouria, b, Susumu Hiragab, Yuki Yanagawab, Yukari Sunoharaa, Hiroshi Matsumotoa, Setsuko Komatsua,
Characterization of calnexin in soybean roots and hypocotyls under osmotic stress
Phytochemistry Volume 74, February 2012, Pages 20–29
 
  TopTip TiO2 2012 Pierce A, Williamson A, Jaworska E, Griffiths JR, Taylor S, et al.
Identification of Nuclear Protein Targets for Six Leukemogenic Tyrosine Kinases Governed by Post-Translational Regulation.
PLoS ONE 7(6): e38928. doi:10.1371/journal.pone.0038928, (2012)
 
      2012 Chen, D. and Caruso, R. A.
Recent Progress in the Synthesis of Spherical Titania Nanostructures and Their Applications.
Adv. Funct. Mater.. doi: 10.1002/adfm.201201880 (2012)
 
      2012 Xing Liu, Lana Barkawi, Gary Gardner and Jerry D. Cohen
Transport of Indole-3-Butyric Acid and Indole-3-Acetic Acid in Arabidopsis Hypocotyls Using Stable Isotope Labeling
Plant Physiology April 2012 vol. 158 no. 4 1988-2000
 
      2012 Xing Liu
Roles of Multiple Mechanisms in Regulating Auxin Levels during Plant Growth and Development
A DISSERTATION SUBMITTED TO THE FACULTY OF THE GRADUATE SCHOOL OF THE UNIVERSITY OF MINNESOTA
Julian Avila, Oliver G. Gregory, Dongyin Su, Taunya A. Deeter, Sixue Chen, Cecilia Silva-Sanchez, Shouling Xu, Gregory B. Martin and Timothy P. Devarenne
The β-Subunit of the SnRK1 Complex Is Phosphorylated by the Plant Cell Death Suppressor Adi31
Plant Physiology July 2012 vol. 159 no. 3 1277-1290
 
      2012 Schneider, Tobias (2012): Mass spectrometric analysis of global histone modification profiles during xenopus laevis development. Dissertation, LMU München: Medizinische Fakultät Thesis  
      2012 Hersberger, Katherine E.
Metal-Oxide Enrichment and Gas-Phase Characterization of Sulfopeptides using Fourier Transform Ion Cyclotron Resonance Mass Spectrometry.
A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy (Chemistry) in The University of Michigan
CHARACTERIZATION OF SULFOPEPTIDES USING FOURIER. TRANSFORM ION CYCLOTRON RESONANCE MASS
      2011 Richard Y-C. Huang, Evelyn M. Kanter, Henry W. Rohrs, Kathryn A. Yamada, and R. Reid Townsend
Identification of phosphorylation sites in the myocardial gap junction protein connexin45.
ASMS 2011poster
 
      2011 Ales Tichy, Barbora Salovska, Pavel Rehulka, Jana Klimentova, Jirina Vavrova, Jiri Stulik, Lenka HernychovaPhosphoproteomics: Searching for a needle in a haystack,
Journal of Proteomics Volume 74, Issue 12, 18 November 2011, Pages 2786–2797
"The filtrates were desalted by using a NuTipHypercarbon (Glygen Corp., Columbia, MD)..."
      2011 Thomas Eickner, Stefan Mikkat, Peter Lorenz, Martin Sklorz, Ralf Zimmermann, Hans-Jürgen Thiesen and Michael O. Glocker
Systematic studies on TiO2-based phosphopeptide enrichment procedures upon in-solution and in-gel digestions of proteins. Are there readily applicable protocols suitable for matrix-assisted laser desorption/ionization mass spectrometry-based phosphopeptide stability estimations?
Eur. J. Mass Spectrom. 17, 507–523 (2011)
"...the polysaccharides were desalted using a NuTip type P20 carbon solid-phase extraction pipette tip (Glygen, USA)."
  TopTip TiO2 2011 Junmin Pan, Bianca Naumann-Busch , Liang Wang , Michael Specht , Martin Scholz , Kerstin Trompelt , and Michael Hippler
Protein Phosphorylation Is a Key Event of Flagellar Disassembly Revealed by Analysis of Flagellar Phosphoproteins during Flagellar Shortening in Chlamydomonas
J. Proteome Res., 2011, 10 (8), pp 3830–3839
 
      2011 Richard Y-C. Huang, James G. Laing, Evelyn M. Kanter, Viviana M. Berthoud, Mingwei Bao, Henry W. Rohrs, R. Reid Townsend, and Kathryn A. Yamada
Identification of CaMKII Phosphorylation Sites in Connexin43 by High-Resolution Mass Spectrometry
J Proteome Res. 2011 March 4; 10(3): 1098–1109.
 
    TiO2 2011 Armann Andaya, Weitao Jia, Masaaki Sokabe, Christopher S. Fraser, John W. B. Hershey, and Julie A. Leary
Phosphorylation of Human Eukaryotic Initiation Factor 2γ: Novel Site Identification and Targeted PKC Involvement
J. Proteome Res., 2011, 10 (10), pp 4613–4623
 
  NuTip Carbon 2011 Bieke Nagels, Francis Santens, Koen Weterings, Els J. M. Van Damme, Nico Callewaert
Improved sample preparation for CE-LIF analysis of plant N-glycans
ELECTROPHORESISSpecial Issue: Focus On Glycomics & Glycoproteomics
Volume 32, Issue 24, pages 3482–3490, December 2011
"The released carbohydrates were desalted on a micro-carbon column from Glygen essentially following the vendor's protocol"
      2011 Takeshi Kumazawa, Chika Hasegawab, Seisaku Uchigasakic, Xiao-Pen Leea, Osamu Suzukid, Keizo Satoa
Quantitative determination of phenothiazine derivatives in human plasma using monolithic silica solid-phase extraction tips and gas chromatography–mass spectrometry
Journal of Chromatography A, Volume 1218, Issue 18, 6 May 2011, Pages 2521–2527
"Top Tips were purchased from Glygen Corp. (Columbia, MD)."
  Empty TopTip   2011 Mariya Liyasovaa, b, Bin Lia, , Lawrence M. Schopfera, Florian Nachonc,   Patrick Massonc, a, Clement E. Furlongd, Oksana Lockridgea Exposure to tri-o-cresyl phosphate detected in jet airplane passengers
Toxicology and Applied Pharmacology, Volume 256, Issue 3, 1 November 2011, Pages 337–347
 
  NuTip C18 2011 Lisa M. Jones , Justin B. Sperry , James A. Carroll , and Michael L. Gross
Fast Photochemical Oxidation of Proteins for Epitope Mapping
Anal. Chem., 2011, 83 (20), pp 7657–7661
"After digestion, an aliquot of digested peptides and glycopep- tides was applied to 10 μl immobilized lectin columns (TopTip)."
    TiO2 2011 David A. Goldstrohm, Corey D. Broeckling, Jessica E. Prenni, and Norman P. Curthoys
Importance of Manual Validation for the Identification of Phosphopeptides Using a Linear Ion Trap Mass
spectrometer
J Biomol Tech. 2011 April; 22(1): 10–20.
"...was resuspended in binding solution (15 mM ammonium acetate, pH 3.5, with 85% ACN) and loaded onto a hydrophilic interaction TopTip HILIC column"
      2011 Takashi Nishikaze, Toshio Nakamura, Hiroshi Jinmei, Junko Amano.
Negative-ion MALDI-MS2 for discrimination of α2,3- and α2,6-sialylation on glycopeptides labeled with a pyrene derivative.
Journal of Chromatography B, Volume 879, Issues 17–18, 15 May 2011, Pages 1419–1428
"The purification was performed using commercial tips filled with anion-exchange resin."
  Coated plates TiO2 2011 Ramesh N. Reddy, Taylor L. Knotts, Brian R. Roberts, Jeffery D. Molkentin, S. Russ Price, Jennifer L. Gooch
Calcineurin A-β is required for hypertrophy but not matrix expansion in the diabetic kidney
Journal of Cellular and Molecular Medicine
Volume 15, Issue 2, pages 414–422, February 2011
"The last purification method used involved anion-exchange spin columns (TopTip..., [Glygen]...)"
  NuTip C18 2011 Yoshiaki Furukawa, Kumi Kaneko, Shoji Watanabe, Koji Yamanaka, and Nobuyuki Nukina
A Seeding Reaction Recapitulates Intracellular Formation of Sarkosyl-insoluble Transactivation Response Element (TAR) DNA-binding Protein-43 Inclusions.
THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 286, NO. 21, pp. 18664–18672, May 27, 2011
"SDS removal protocol using TopTip filters supplied with HILIC resin..."
      2011 Jung Hun Oh, Jeffrey M. Craft, Reid Townsend,Joseph O. Deasy, Jeffrey D. Bradley, and Issam El NaqaA Bioinformatics Approach for Biomarker Identification in Radiation-Induced Lung Inflammation from Limited Proteomics Data.
J Proteome Res. 2011 March 4; 10(3): 1406–1415. doi:10.1021/pr101226q.
"...peptide mass fingerprints produced by the automatic work- flow performed equally well or better than the manual process regarding signal intensity and protein sequence coverage. As an additional benefit, the whole process is carried out in a reproducible and timesaving manner."
  NuTip Carbon 2011 David A. Sela, Yanhong Li, Larry Lerno, Shuai Wu, Angela M. Marcobal, J. Bruce German, Xi Chen, Carlito B. Lebrilla and David A. Mills.
An Infant-associated Bacterial Commensal Utilizes Breast Milk Sialyloligosaccharides
THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 286, NO. 14, pp. 11909–11918, April 8, 2011
 
    TiO2 2011 M. N. Schulz, J. Fanghänel, M. Schäfer, V. Badock, H. Briem, U. Boemer, D. Nguyen, M. Husemann and R. C. Hillig
A crystallographic fragment screen identifies cinnamic acid derivatives as starting points for potent Pim-1 inhibitors
Acta Cryst. (2011). D67, 156-166
 
    TiO2/ZrO2 Mix 2011 Katerina Tsougeni , Panagiotis Zerefos , Angeliki Tserepi , Antonia Vlahou , Spiros D. Garbis and Evangelos Gogolides
TiO2–ZrO2 affinity chromatography polymeric microchip for phosphopeptide enrichment and separation
Lab Chip, 2011,11, 3113-3120
 
    C18 2011 Yoshiaki Furukawa, Kumi Kaneko§, and Nobuyuki Nukina
Tau Protein Assembles into Isoform- and Disulfide-dependent Polymorphic Fibrils with Distinct Structural Properties
THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 286, NO. 31, pp. 27236–27246, August 5, 2011
 
    TiO2 2011 Alexandra M. E. Jones, Thomas S. Nühse
Phosphoproteomics Using iTRAQ Plant Kinases
Methods in Molecular Biology Volume 779, 2011, pp 287-302
 
  TopTip TiO2 2011 Jesse Rinehart, Norma Va´zquez, Kristopher T. Kahle, Caleb A. Hodson, Aaron M. Ring, Erol E. Gulcicek, Angeliki Louvi, Norma A. Bobadilla, Gerardo Gamba, and Richard P. Lifton
WNK2 Kinase Is a Novel Regulator of Essential Neuronal Cation-Chloride Cotransporters
THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 286, NO. 34, pp. 30171–30180, August 26, 2011
 
      2011 Claudia Röwer, Cornelia Koy, Michael Hecker, Toralf Reimer, Bernd Gerber, Hans-Jürgen Thiesen, Michael O. Glocker
Mass Spectrometric Characterization of Protein Structure Details Refines the Proteome Signature for Invasive Ductal Breast Carcinoma
Journal of The American Society for Mass Spectrometry: March 2011, Volume 22, Issue 3, pp 440-456
 
  NuTip C18 2011 Setsuko Komatsu, Deschamps Thibaut, Susumu Hiraga, Mikio Kato, Mitsuru Chiba, Akiko Hashiguchi, Makoto Tougou,Satoshi Shimamura, Hiroshi Yasue
Characterization of a novel flooding stress-responsive alcohol dehydrogenase expressed in soybean roots
Plant Mol Biol (2011) 77:309–322
 
    C18 2011 Valentina Calamia, Beatriz Rocha, Jesús Mateos, Patricia Fernández-Puente, Cristina Ruiz-Romero, and Francisco J. Blanco
Metabolic Labeling of Chondrocytes for the Quantitative Analysis of the Interleukin-1-beta-mediated Modulation of Their Intracellular and Extracellular Proteomes
J. Proteome Res., 2011, 10 (8), pp 3701–3711
 
      2011 Catherine K. Foo, Yumiko Ohhashi, Mark J.S. Kelly, Motomasa Tanaka, Jonathan S. Weissman,
Radically Different Amyloid Conformations Dictate the Seeding Specificity of a Chimeric Sup35 Prion Journal of Molecular Biology
Volume 408, Issue 1, 22 April 2011, Pages 1–8
 
  NuTip HILIC 2011 Yasuhiro Katsumata, Yasushi Kawaguch, Sayumi Baba, Seisuke Hattor, Koji Tahara, Kaori Ito, Tadao Iwasaki, Nozomi Yamaguchi, Masaaki Oyama, Hiroko Kozuka-Hata, Hiroaki Hattori, Kinya Nagata, Hisashi Yamanaka, and Masako Hara
Identification of Three New Autoantibodies Associated with Systemic Lupus Erythematosus Using Two Proteomic
Approaches
June 1, 2011 Molecular & Cellular Proteomics, 10, M110.005330.
 
      2011 Barbara S. Sixt, Christian Heinz, Peter Pichler, Eva Heinz, Jacqueline Montanaro,, Huub J. M. Op den Camp, Gustav Ammerer, Karl Mechtler, Michael Wagner, Matthias Horn
Proteomic analysis reveals a virtually complete set of proteins for translation and energy generation in elementary bodies of the amoeba symbiont Protochlamydia amoebophila
PROTEOMICS Volume 11, Issue 10, pages 1868–1892, May 2011
 
      2011 Emdadul Haque, Md.; Kawaguchi, Kentaro; Komatsu, Setsuko
Analysis of Proteins in Aerenchymatous Seminal Roots of Wheat Grown in Hypoxic Soils Under Waterlogged Conditions (supplementary Material)
Protein and Peptide Letters, Volume 18, Number 9
Analysis of Proteins in Aerenchymatous Seminal Roots of Wheat Grown in Hypoxic Soils
Under Waterlogged Conditions
  TwistTip SAX 2011 Xing Liu, Jerry D. Cohen and Gary Gardner
Low-Fluence Red Light Increases the Transport and Biosynthesis of Auxin1
Plant Physiology October 2011 vol. 157 no. 2 891-904
 
      2011 Haijun Liua, Richard Y.-C. Huangb, Jiawei Chenb, Michael L. Grossb, and Himadri B. Pakrasia,
Psb27, a transiently associated protein, binds to the chlorophyll binding protein CP43 in photosystem II assembly intermediates
10.1073/pnas.1111597108 . PNAS November 8, 2011 vol. 108
 
      2011 Nathan P. Mane, Li Don, Weidong Zhou§, Xiuxia D, Nikitha Regh, Arjan C. Koo, Dahan Cho, Charles L. Bailey, Emanuel F. Petricoin , Lance A. Liotta and Serguei G. Popov
Discovery of Mouse Spleen Signaling Responses to Anthrax using Label-Free Quantitative Phosphoproteomics via Mass Spectrometry
March 1, 2011 Molecular & Cellular Proteomics, 10, M110.000927.
 
  Nutip C18 2011 Luc Bonnefonda, Taiga Araib, Yuriko Sakaguchib, Tsutomu Suzukib, Ryuichiro Ishitania, and Osamu Nurekia
tRNA-dependent peptide bond formation by the transferase PacB in biosynthesis of the pacidamycin group of pentapeptidyl nucleoside antibiotics
Proc. Natl. Acad. Sci. USA 2011 108 (30) 12249-12253
 
      2011 Eric S. Underbakke, Yimin Zhu, Laura L. Kiessling
Protein Footprinting in a Complex Milieu: Identifying the Interaction Surfaces of the Chemotaxis Adaptor Protein CheW
Journal of Molecular Biology Volume 409, Issue 4, 17 June 2011, Pages 483–495
 
      2011 T. Shinanoa, S. Komatsub, T. Yoshimurac, S. Tokutakec, F.-J. Kongb, T. Watanabec, J. Wasakid, M. Osaki
Proteomic analysis of secreted proteins from aseptically grown rice
Phytochemistry Volume 72, Issues 4–5, April 2011, Pages 312–320
 
      2011 Naohiro Terasaka, Satoshi Kimura, Takuo Osawa, Tomoyuki Numata & Tsutomu Suzuki
Biogenesis of 2-agmatinylcytidine catalyzed by the dual protein and RNA kinase TiaS
Nature Structural & Molecular Biology Volume: 18, Pages: 1268–1274 Year published: (2011)
 
      2011 Deepak B Thimiri Govinda Raj, Bart Ghesquière, Arun Kumar Tharkeshwar, Katrijn Coen, Rita Derua, Dieter Vanderschaeghe, Evelien Rysman, Murali Bagadi, Pieter Baatsen, Bart De Strooper, Etienne Waelkens, Gustaaf Borghs, Nico Callewaert, Johan Swinnen, Kris Gevaert & Wim Annaert
A novel strategy for the comprehensive analysis of the biomolecular composition of isolated plasma membranes
Molecular Systems Biology 7 Article number: 541 doi:10.1038/msb.2011.74
 
  TopTip Carbon 2011 Schneider TD, Arteaga-Salas JM, Mentele E, David R, Nicetto D, et al.
Stage-Specific Histone Modification Profiles Reveal Global Transitions in the Xenopus Embryonic Epigenome.
PLoS ONE 6(7): e22548. doi:10.1371/journal.pone.0022548 (2011)
 
      2011 KL Krivos
Analysis of RNA: Peptide Heteroconjugates by Electron Induced Dissociation Mass Spectrometry
- 2011 - etd.ohiolink.edu
Development of Techniques for Removal of Sodium Dodecyl Sulfate for Enhanced Protein Identification and Characterization
S Fitzsimmons - 2011 - dalspace.library.dal.ca
 
      2011 Zhou, Wen
Gas-phase Ion-electron and Ion-photon Reactions for Structural Characterization of Protein Glycosylation.
A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy
(Chemistry)
in The University of Michigan
Gas-phase Ion-electron and Ion-photon Reactions for Structural. Characterization of Protein Glycosylation
  NuTip TiO2 2010 Uma K. Aryal and Andrew R.
Enrichment and analysis of phosphopeptides under different experimental conditions using titanium dioxide affinity chromatography and mass spectrometry
Rapid Communications in Mass Spectrometry, Volume 24, Issue 2, pages 219–231, 30 January 2010
"After HPLC separation, the phosphorylated glycans were purified using ion-exchange resin Muromac and applied to porous graphitic carbon (PGC) cartridges (Nutip Carbon and GL-Pak Carbograph) for desalting"
  NuTip C18 2011 Setsuko Komatsu , Akifumi Yamamoto , Takuji Nakamura , Mohammad-Zaman Nouri , Yohei Nanjo , Keito Nishizawa , and Kiyoshi Furukawa
Comprehensive Analysis of Mitochondria in Roots and Hypocotyls of Soybean under Flooding Stress using Proteomics and Metabolomics Techniques
J. Proteome Res., 2011, 10 (9), pp 3993–4004
 
Year Molecule Application Publication Link Quote Glygen product Media
2010 Glyco-peptide Enrichment Susan Grass, Cheryl F. Lichti, R. Reid Townsend, Julia Gross, and Joseph W. St. Geme, III The Haemophilus influenzae HMW1C Protein Is a Glycosyltransferase That Transfers Hexose Residues to Asparagine Sites in the HMW1 Adhesin PLoS Pathog. 2010 May; 6(5): e1000919. Click "Peptides were acidified with 5.5 цl formic acid (Sigma) and extracted 6 times with 10–200 цl NuTip
porous graphite carbon wedge tips (Glygen) according to the manufacturer's directions and were
then eluted into 1.5 ml autosampler vials with 60% acetonitrile"
NuTip HyperCarb
2010 Glyco-protein Purification / desalting Hiroto Hirayama, Junichi Seino, Toshihiko Kitajima, Yoshifumi Jigami and Tadashi Suzuki Free Oligosaccharides to Monitor Glycoprotein Endoplasmic Reticulum-associated Degradation in Saccharomyces cerevisiae The Journal of Biological Chemistry,   285,  12390-12404. Click "For desalting of PA-oligosaccharide-containing fractions, we used
C18+ carbon NuTip (Hypercrb, Glygen, Columbia, MD) as described previously"
NuTip C18+HyperCarb
2010 Glyco-protein Enrichment Mizuho INAGAKI, Shuuichi NAKAYA, Daisuke NOHARA, Tomio YABE, Yoshihiro KANAMARU and Tohru SUZUKI, “The Multiplicity of N-Glycan Structures of Bovine Milk 18 kDa Lactophorin (Milk GlyCAM-1)”, Biosci. Biotechnol. Biochem., Vol. 74, 447-450 (2010) . Click "The PA-derivatized glycans were then dried with a centrifugal evaporator and dissolved
in 10 цl of 0.1% v/v trifluoroacetic acid, followed by purified via Nutip Carbon (Glygen,
Columbia, MD) according to the manufacturer's protocol."
NuTip HyperCarb
2010 Glyco-protein Enrichment  John W. Froehlich, Eric D. Dodds, Mariana Barboza, Erica L. McJimpsey, Richard R. Seipert, Jimi Francis, Hyun Joo An, Samara Freeman, J. Bruce German, Carlito B. Lebrilla Glycoprotein Expression in Human Milk during Lactation  Journal of Agricultural and Food Chemistry 2010 58 (10), 6440-6448 Click "Porous graphitized carbon cartridges were obtained from Glygen. " NuTip HyperCarb
2010 Glyco-protein Enrichment Ming Lei, et al, 'Sequential Enrichment of Sulfated Glycans by Strong Anion-Exchange Chromatography Prior to Mass Spectrometric Measurements,' J Am Soc Mass Spectrom, 2010, 21, 348-357 Click "Sequential enrichment of sulfated glycans is attained using spin-columns packed
with strong anion exchanger"
TopTip SAX
2010 Peptide Purification / desalting Fan-Jiang Konga, Atsushi Oyanagia and Setsuko Komatsu Cell wall proteome of wheat roots under flooding stress using gel-based and LC MS/MS-based proteomics approaches Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics
Volume 1804, Issue 1, January 2010, Pages 124-136
Click "The peptide solution was desalted with NuTip C-18 pipet tips (Glygen, Columbia, MD, USA)." NuTip C18
2010 Peptide Purification / desalting Mohammad-Zaman Nouri, Setsuko Komatsu Comparative analysis of soybean plasma membrane proteins under osmotic stress using gel-based and LC MS/MS-based proteomics approaches PROTEOMICS Volume 10, Issue 10, pages 1930–1945, No. 10 May 2010 Click "The resulting peptides were concentrated and desalted using a NuTip C-18 pipet tips (Glygen, Columbia, MD, USA) and analyzed by nanoLC MS/MS for protein identification." NuTip C18
2010 Peptide Purification / desalting Jian-Hua XIONG, Bin-Ying FU, Hua-Xue XU, and Yang-Sheng LI, Proteomic analysis of PEG-simulated drought stress responsive proteins of rice leaves using a pyramiding rice line at the seedling stage Botanical Studies (2010) 51: 137-145. Click "The peptide solution thus obtained was dried and reconcentrated with 30 ml of 0.1% TFA in 5% ACN/water and then desalted with NuTip C-18 pipette tips (Glygen, USA)." NuTip C18
2010 Peptide Purification / desalting Afroz, Amber; Rashid Khan, Muhammad; Komatsu, Setsuko Determination of Proteins Induced in Response to Jasmonic Acid and Salicylic Acid in Resistant and Susceptible Cultivars of Tomato Protein and Peptide Letters, Volume 17, Number 7, July 2010 , pp. 836-846(11) Click "The resulting peptide solution was dried and reconstituted with 30 μL of 0.1% trifluoroacetic acid
in 5% acetonitrile and then desalted with NuTip C18 pipette tips (Glygen, Columbia, MD, USA)."
NuTip C18
2010 Peptide Purification / desalting Afroz, Amber; Hashiguchi, Akiko; Khan, Muhammad R.; Komatsu, Setsuko Analyses of the Proteomes of the Leaf, Hypocotyl, and Root of Young Soybean Seedlings Protein and Peptide Letters, Volume 17, Number 3, March 2010 , pp. 319-331(13) Click "The resulting peptide solution was dried and reconstituted with 30 μL of 0.1% trifluoroacetic acid
in 5% acetonitrile and then desalted with NuTip C18 pipette tips (Glygen, Columbia, MD)"
NuTip C18
2010 Peptide Purification / desalting Yoshiaki Furukawa, Kumi Kaneko, Koji Yamanaka and Nobuyuki Nukina Mutation-dependent polymorphism of Cu,Zn-superoxide dismutase aggregates in the familial form of amyotrophic lateral sclerosis Journal of Biological Chemistry April 19, 2010 Click "...desalted using NuTip C-18 (Glygen Co.)" NuTip C18
2010 Peptide Purification

N Ahsan, Y Nanjo, H Sawada, Y Kohno, S Komatsu. Ozone stress-induced proteomic changes in leaf total soluble and chloroplast proteins of soybean reveal that carbon allocation is involved in adaptation in the early developmental stage. PROTEOMICS, Volume 10, Issue 14, pages 2605–2619, No. 14 July 2010

Click

"The generated peptides were purified using a NuTip C-18 (Glygen, Columbia, MD, USA)."

NuTip C18
2010 Peptide Purification Junsong Sun, Robert C. Hopkins, Francis E. Jenney, Jr, Patrick M. McTernan, and Michael W. W. Adams Heterologous Expression and Maturation of an NADP-Dependent [NiFe]-Hydrogenase: A Key Enzyme in Biofuel Production PLoS One. 2010; 5(5): e10526. Click "The peptides were purified with C-18 reversed-phase NuTip® cartridges according to the manufacturer's instructions (Glygen Corp., Columbia, MD). " NuTip C18
2010 Peptide Purification Hamid Sobhanian, Roya Razavizadeh, Yohei Nanjo, Ali Akbar Ehsanpour, Ferdous Rastgar Jazii,
Nasrin Motamed, Setsuko Komatsu Proteome analysis of soybean leaves, hypocotyls and roots under salt stress Proteome Science 2010, 8:19.
Click "The generated peptides were purified
using NuTip C-18 columns (Glygen Corp., Columbia,
MD, USA)."
NuTip C18
2010 Peptide Purification A Cvetkovic, AL Menon, MP Thorgersen, JW Scott, et al Microbial metalloproteomes are largely uncharacterized Nature 466, 779-782 (5 August 2010) Click "The peptides were purified with C-18 reversed-phase NuTip cartridges according to the manufacturer's instructions (Glygen). " NuTip C18
2010 Peptide Purification Thalia Nittis, Lionel Guittat, Richard D. LeDuc, Ben Dao, Julien P. Duxin, Henry Rohrs, R. Reid Townsend and Sheila A. Stewart. Revealing Novel Telomere Proteins Using in Vivo Cross-linking, Tandem Affinity Purification, and Label-free Quantitative LC-FTICR-MS  Mol Cell Proteomics 2010 9: 1144-1156. Click "The final eluates were forced through Filter-in-a-tips (Glygen Corp., Columbia, MD) to remove any remaining beads." TopTip Filter
2010 Peptide Enrichment Ventura CL,  Malachowa N,  Hammer CH,  Nardone GA,  Robinson MA,  et al. 2010 Identification of a Novel Staphylococcus aureus  Two-Component Leukotoxin Using Cell Surface Proteomics. PLoS ONE 5(7): e11634. doi:10.1371/journal.pone.0011634  Click "The dried sample was dissolved in 50 µl of 50 mM formic acid/75% isopropanol and applied to polyhydroxyethyl A 10-µl solid phase extraction tip (Glygen Corporation, Columbia, MD). " NuTip Poly-Hydroxy-ethyl A
2010 Peptide Enrichment Wen-Ping Chen, Xiao-Yuan Yang, Adrian D. Hegeman, William M. Gray, and Jerry D. Cohen Microscale analysis of amino acids using gas chromatography–mass spectrometry after methyl chloroformate derivatization Journal of Chromatography B
Volume 878, Issue 24, 15 August 2010, Pages 2199-2208
Click "Alternatively, commercial SCX tips (TT2-TWSCX.96, Glygen Corp, Columbia, MD, USA), based on their modified Top-tip format, proved successful and are more convenient for routine use." TopTip SCX
2010 Peptide Enrichment Scott J Walmsley, Corey Broeckling, Ann Hess, Jessica Prenni,  and Norman P. Curthoys Proteomic Analysis of Brush Border Membrane Vesicles Isolated from Purified Proximal Convoluted Tubules Am J Physiol Renal Physiol (March 10, 2010). doi:10.1152/ajprenal.00711.2009 Click "For SCX fractionation, the peptides were bound to a 156 Polysulfoethyl-A microspin TopTip
(Glygen) and eluted stepwise with 20 μl volumes of 157 increasing ammonium acetate
(20, 40, 60, 80, 120, 160 and 200 mM) in 20% acetonitrile, pH 158"
TopTip SSA
2010 Phospho-peptide Enrichment Vishram P. Kedar, Martyn K. Darby, Jason G. Williams, and Perry J. Blackshear Phosphorylation of Human Tristetraprolin in Response to Its Interaction with the Cbl Interacting Protein CIN85 PLoS One. 2010; 5(3): e9588. Click "For phosphopeptide enrichment, TiO2 tips (Glygen) were employed using essentially the manufacturer's recommended protocol." NuTip TiO2
2010 Phospho-peptide Enrichment Mikkat S, Lorenz P, Scharf C, Yu X, Glocker MO, Ibrahim SM. MS characterization of qualitative protein polymorphisms in the spinal cords of inbred mouse strains. Proteomics. 2010 Mar;10(5):1050-62. Click "Porous titanium dioxide immobilized to the inner surface of pipette tips (NuTipTM, Glygen, Columbia, MD, USA) was used for the selective enrichment of phosphorylated peptides. " NuTip TiO2
2010 Phospho-peptide Enrichment John D. Leszyk Evaluation of the New MALDI Matrix 4-Chloro-α-Cyanocinnamic Acid J Biomol Tech. 2010 July; 21(2): 81–91. Click "Phosphopeptides were enriched using a modification of the published method.7 The dried peptide digest was dissolved in 10 μl binding solvent (75:19:6 acetonitrile:water:TFA). A 1- to 10-μl TiO2  NuTip was washed two times with 10 μl aliquots of binding solvent. Phosphopeptides were bound by repetitive pipetting (20 times) of the 10-μl sample volume. The NuTip was then washed five times with binding solvent and then five times with 0.1% TFA. Phosphopeptides were then eluted in a separate tube with a 10-μl vol 6% NH4OH after repetitive pipetting (10 times). A 5-μl aliquot of 5% TFA was then added, and the phosphopeptides were concentrated further and purified prior to MALDI analysis using a micro C18 ZipTip." NuTip TiO2
2010 Phospho-peptide Enrichment Matthew B. Gatesa, Kenneth B. Tomera and Leesa J. Deterding. Comparison of Metal and Metal Oxide Media for Phosphopeptide Enrichment Prior to Mass Spectrometric Analyses. JASMS June 2010. Click "Both MALDI and ESI-MS analyses of the TiO2 NuTip eluents resulted in the reproducible observation of a greater number of unique sites of phosphorylation with the least amount of nonspecific binding compared with the other MOAC resins. ... Enrichment procedures before MS analyses dramatically improve detection and sequencing of phosphopeptides..." NuTip TiO2
2010 Phospho-peptide Enrichment Uma K. Aryal, Andrew R. S. Ross. Enrichment and analysis of phosphopeptides under different experimental conditions using titanium dioxide affinity chromatography and mass spectrometry. Rapid Communications in Mass Spectrometry Volume 24, Issue 2, pages 219–231, 30 January 2010. Click "To address these uncertainties, we have evaluated the performance of TiO2-MOAC under different
loading and elution conditions, using a single make and model of commercially available TiO2
column (NuTipTM NT1TIO, Glygen Corp., USA) to minimize experimental variability."
NuTip TiO2
2010 Phospho-peptide Enrichment Jamie D. Dunn, Gavin E. Reid, Merlin L. Bruening Techniques for phosphopeptide enrichment prior to analysis by mass spectrometry Mass Spectrometry Reviews, Volume 29, Issue 1, pages 29–54, January 2010 Click "...Microtips that contained zirconium dioxide (Glygen, Columbia, MD) were utilized for the enrichment of phosphorylated peptides, and the specificity and recovery achieved was compared to that obtained with Glygen microtips containing titanium dioxide (with the same binding, rinsing and elution solutions)... Overall, the TiO2 microtips were more selective for enrichment of multiply phosphorylated peptides, whereas the ZrO2 tips enriched primarily monophosphorylated peptides. " NuTip and TopTip TiO2
2010 Phospho-peptide Enrichment Parvathi Rudrabhatla, Philip Grant, Howard Jaffe, Michael J. Strong,  and Harish C. Pant Quantitative phosphoproteomic analysis of neuronal intermediate filament proteins (NF-M/H) in Alzheimer’s disease by iTRAQ FASEB J..2010; 0: fj.10-157859v1 Click "...phopeptide enrichment by TiO2 chromatography utilizing a 1- to 200-l TiO2 Top
Tip (Glygen Corp., Columbia, MD, USA) essentially according to the method of Wu
et al. "
TopTip TiO2
2010 Phospho-peptide Enrichment Özlü, Nurhan and Monigatti, Flavio and Renard, Bernhard Y. and Field, Christine M. and Steen, Hanno and Mitchison, Timothy J. and Steen, Judith J. Binding Partner Switching on Microtubules and Aurora-B in the Mitosis to Cytokinesis Transition Mol Cell Proteomics 2010 9: 336-350. First Published on September 28, 2009, doi:10.1074/mcp.M900308-MCP200 Click "TiO 2 was used to enrich for phosphopeptides. Briefly, lyophilized samples were dissolved
in binding buffer (5% TFA, 40% acetonitrile). After loading samples onto the TiO 2 columns
(TopTip, Glygen), the column was washed with the binding buffer."
TopTip TiO2
2010 Phospho-peptide Enrichment Weitao Jia, Justin F. Shaffer, Samantha P. Harris, Julie A. Leary Identification of Novel Protein Kinase A Phosphorylation Sites in the M-domain of Human and Murine Cardiac Myosin Binding Protein-C Using Mass Spectrometry Analysis Journal of Proteome Research  2010  9  (4), 1843-1853 Click "The phosphopeptides were also enriched using TopTips filled with TiO 2 (particle size 20−30 μm, Glygen Corp., Columbia, MD) as described in the manufacturer's instruction with minor modifications." TopTip TiO2
2010 Phospho-peptide Enrichment Michael Mazanek, Elisabeth Roitinger, Otto Hudecz, James R.A. Hutchins, Bjorn Hegemann, Goran Mitulovic, Thomas Taus, Christoph Stingl, Jan-Michael Peters, Karl Mechtler, A new acid mix enhances phosphopeptide enrichment on titanium- and zirconium dioxide for mapping of phosphorylation sites on protein complexes, Journal of Chromatography B, Volume 878, Issues 5-6, 15 February 2010, Pages 515-524. Click     TiO2/ZrO2
2010 Protein Purification Wim Fremout, Maarten Dhaenens, Steven Saverwyns, Jana Sanyova, Peter Vandenabeele, Dieter Deforce, Luc Moens, Tryptic peptide analysis of protein binders in works of art by liquid chromatography-tandem mass spectrometry, Analytica Chimica Acta, Volume 658, Issue 2, 25 January 2010, Pages 156-162. Click   TopTip HILIC

 

 

PUBLICATIONS BY APPLICATION

 

Molecule Application Year Publication Link Quote Glygen product Media
Peptide Enrichment 2010 Ventura CL,  Malachowa N,  Hammer CH,  Nardone GA,  Robinson MA,  et al. 2010 Identification of a Novel Staphylococcus aureus  Two-Component Leukotoxin Using Cell Surface Proteomics. PLoS ONE 5(7): e11634. doi:10.1371/journal.pone.0011634  Click "The dried sample was dissolved in 50 µl of 50 mM formic acid/75% isopropanol and applied to polyhydroxyethyl A 10-µl solid phase extraction tip (Glygen Corporation, Columbia, MD). " NuTip Poly-Hydroxy-ethyl A
Peptide Enrichment 2010 Wen-Ping Chen, Xiao-Yuan Yang, Adrian D. Hegeman, William M. Gray, and Jerry D. Cohen Microscale analysis of amino acids using gas chromatography–mass spectrometry after methyl chloroformate derivatization Journal of Chromatography B
Volume 878, Issue 24, 15 August 2010, Pages 2199-2208
Click "Alternatively, commercial SCX tips (TT2-TWSCX.96, Glygen Corp, Columbia, MD, USA), based on their modified Top-tip format, proved successful and are more convenient for routine use." TopTip SCX
Peptide Enrichment 2010 Scott J Walmsley, Corey Broeckling, Ann Hess, Jessica Prenni,  and Norman P. Curthoys Proteomic Analysis of Brush Border Membrane Vesicles Isolated from Purified Proximal Convoluted Tubules Am J Physiol Renal Physiol (March 10, 2010). doi:10.1152/ajprenal.00711.2009 Click "For SCX fractionation, the peptides were bound to a 156 Polysulfoethyl-A microspin TopTip
(Glygen) and eluted stepwise with 20 μl volumes of 157 increasing ammonium acetate
(20, 40, 60, 80, 120, 160 and 200 mM) in 20% acetonitrile, pH 158"
TopTip SSA
Peptide Enrichment 2009 Fang Shen, Nan Li, Padmaja Gade, Dhananjaya V. Kalvakolanu, Timothy Weibley, Brad Doble, James R. Woodgett, Troy D. Wood, and Sarah L. Gaffen, IL-17 Receptor Signaling Inhibits C/EBPβ by Sequential Phosphorylation of the Regulatory 2 Domain, Sci. Signal., 24 February 2009 Vol. 2, Issue 59, p. ra8 Click "…Vacuum-dried peptides were dissolved in 80%ACN/0.1%TFA and loaded on a Top tip-TiO 2 micropipette tip (Glygen Corp)" TopTip TiO2
Peptide Enrichment 2009 Proteomic analyses of native brain KV4.2 channel complexes. Céline Marionneau, Richard D. LeDuc, Henry W. Rohrs, Andrew J. Link, R. Reid Townsend, and Jeanne M. Nerbonne.  Proteomic analyses of native brain KV4.2 channel complexes. 2009 Jul–Aug; 3(4): 284–294.Published online 2009 July 16. Click "Peptides were acidified with formic acid, extracted with NuTip porous graphite carbon wedge tips (Glygen)…" NuTip HyperCarb
Peptide Enrichment 2008 R . Craig-Schapiro, A . Davis, R . LeDuc, A . Sha , D . Holtzman, R . Townsend , A . Fagan: Development of a solid phase extraction protocol for extracting peptides from cerebrospinal fluid in conjunction with tandem mass spectrometry to identify novel biomarkers for Alzheimer's disease Alzheimer's and Dementia , Volume 4, Issue 4, Supplement 1, July 2008, Pages T536-T537.     NuTip Carbon
Peptide Enrichment 2007 Jurre J. Kamphorst,Rob van der Heijden, Jeroen DeGroot, Floris P. J. G. Lafeber, Theo H. Reijmers,Benno van El,Ubbo R. Tjaden, Jan van der Greef, and Thomas Hankemeier, Profiling of Endogenous Peptides in Human Synovial Fluid by NanoLC?MS:  Method Validation and Peptide Identification, J. Proteome Res., 2007, 6 (11), pp 4388–4396. Click "SPE was performed with POROS RP-2 C 18 TopTips (Glygen, Columbia, MD)" NuTip POROS RP2
Peptide Enrichment 2007 Gordon B. Mitchell, Muthafar H. Al-Haddawi, Mary Ellen Clark, Jennifer D. Beveridge, and Jeff L. Caswell. Effect of Corticosteroids and Neuropeptides on the Expression of Defensins in Bovine Tracheal Epithelial Cells. Infection and Immunity, March 2007, p. 1325-1334, Vol. 75, No. 3. Click "Samples were concentrated by C18 TopTip (Glygen Corp., Columbia, MD)" TopTip C18
Peptide Enrichment 2004 Wolfgang H. Fischer, Rebecca Ross, Minkyu Park, Chien Li, Cynthia Donaldson and Joan Vaughan. Identification of Processing Sites in Neuropeptides Employing Microaffinity Columns and MALDI-MS (ASMS 2004). used TopTip with Carboxyl group for the EDC coupling applications     TopTip Affinity
Peptide Enrichment 2004 Andrew J. Alpert and Ashok K. Shukla (ABRF 2004 Poster), Displacement Chromatography Effects Can Cause Highly Selective Sampling of Peptides During Solid Phase Extraction Cleanup. Click      
Peptide Purificadtion 2008 Jing-hua Xi, Fang Bai, Julia Gross , R. Reid Townsend, A. Sue Menko, and Usha P. Andley, A KNOCK-IN MOUSE MODEL DEMONSTRATES THAT THE R49C MUTATION IN A-CRYSTALLIN ENHANCES PROTEIN INSOLUBILITY AND CELL DEATH, J. Biol. Chem., Vol. 283, Issue 9, 5801-5814, February 29, 2008. Click "...digest was diluted with an equal volume of water and the peptides were extracted with a NuTip porous graphite carbon tip (Glygen Part No. NP2CAR.96)" NuTip Carbon
Peptide Purification 2010 N Ahsan, Y Nanjo, H Sawada, Y Kohno, S Komatsu. Ozone stress-induced proteomic changes in leaf total soluble and chloroplast proteins of soybean reveal that carbon allocation is involved in adaptation in the early developmental stage. PROTEOMICS, Volume 10, Issue 14, pages 2605–2619, No. 14 July 2010 Click "The generated peptides were purified using a NuTip C-18 (Glygen, Columbia, MD, USA)." NuTip C18
Peptide Purification 2010 Junsong Sun, Robert C. Hopkins, Francis E. Jenney, Jr, Patrick M. McTernan, and Michael W. W. Adams Heterologous Expression and Maturation of an NADP-Dependent [NiFe]-Hydrogenase: A Key Enzyme in Biofuel Production PLoS One. 2010; 5(5): e10526. Click "The peptides were purified with C-18 reversed-phase NuTip® cartridges according to the manufacturer's instructions (Glygen Corp., Columbia, MD). " NuTip C18
Peptide Purification 2010 Hamid Sobhanian, Roya Razavizadeh, Yohei Nanjo, Ali Akbar Ehsanpour, Ferdous Rastgar Jazii,
Nasrin Motamed, Setsuko Komatsu Proteome analysis of soybean leaves, hypocotyls and roots under salt stress Proteome Science 2010, 8:19.
Click "The generated peptides were purified
using NuTip C-18 columns (Glygen Corp., Columbia,
MD, USA)."
NuTip C18
Peptide Purification 2010 A Cvetkovic, AL Menon, MP Thorgersen, JW Scott, et al Microbial metalloproteomes are largely uncharacterized Nature 466, 779-782 (5 August 2010) Click "The peptides were purified with C-18 reversed-phase NuTip cartridges according to the manufacturer's instructions (Glygen). " NuTip C18
Peptide Purification 2010 Thalia Nittis, Lionel Guittat, Richard D. LeDuc, Ben Dao, Julien P. Duxin, Henry Rohrs, R. Reid Townsend and Sheila A. Stewart. Revealing Novel Telomere Proteins Using in Vivo Cross-linking, Tandem Affinity Purification, and Label-free Quantitative LC-FTICR-MS  Mol Cell Proteomics 2010 9: 1144-1156. Click "The final eluates were forced through Filter-in-a-tips (Glygen Corp., Columbia, MD) to remove any remaining beads." TopTip Filter
Peptide Purification 2009 Roya Razavizadeh, Ali Akbar Ehsanpour, Nagib Ahsan, Setsuko Komatsu, Proteome analysis of tobacco leaves under salt stress, Peptides, Volume 30, Issue 9, September 2009, Pages 1651-1659. Click "Purification of the generated peptides was achieved using NuTip C-18 (Glygen, Columbia, MD, USA)." NuTip C18
Peptide Purification 2009 S. Komatsu, E. Yamada and K. Furukawa Cold stress changes the concanavalin A-positive glycosylation pattern of proteins expressed in the basal parts of rice leaf sheaths, Amino Acids (2009), 0939-4451 (Print) 1438-2199 (Online). Click "Purification of the generated peptides was achieved using Nutip C-18 (Glygen, Columbia, MD, USA). " NuTip C18
Peptide Purification 2009 Setsuko Komatsu, Tetsuya Sugimoto, Tomoki Hoshino, Yohei Nanjo and Kiyoshi Furukawa Identification of flooding stress responsible cascades in root and hypocotyl of soybean using proteome analysis Amino Acids Volume 38, Number 3, 729-738, DOI: 10.1007/s00726-009-0277-0 Click "The degenerated peptides were purified using NuTip C-18 (Glygen, Columbia, MD, USA)." NuTip C18
Peptide Purification 2009 S. Komatsu, E. Yamada and K. Furukawa, Cold stress changes the concanavalin A-positive glycosylation pattern of proteins expressed in the basal parts of rice leaf sheaths, Amino Acids, Volume 36, Number 1 / January, 2009 Click "Purification of the generated peptides was achieved using Nutip C-18 (Glygen, Columbia, MD, USA" NuTip C18
Peptide Purification / desalting 2009 T. Mahmood, A. Jan and S. Komatsu Biologia Plantarum, Volume 53, Number 2 / June, 2009, 285-293, Proteomic analysis of bacterial blight defence signalling pathway using transgenic rice overexpressing thaumatin-like protein. Click "The peptide solution, thus obtained was dried and reconcentrated with 0.03 cm3 of 0.1 TFA in 5 % acetonitrile/water and then desalted with NuTip C-18 pipette tips (Glygen, Columbia, MD, USA)." NuTip C18
Peptide Purification / desalting 2008 Hideaki Shimizu, Asako Tosaki, Kumi Kaneko, Tamao Hisano, Takashi Sakurai, and Nobuyuki Nukina Crystal, Structure of an Active Form of BACE1, an Enzyme Responsible for Amyloid ? Protein Production, Molecular and Cellular Biology, June 2008, p. 3663-3671, Vol. 28, No. 11 Click "desalted with a hydrophilic Nutip cartridge" NuTip HILIC
Peptide Purification / desalting 2008 Extensive contacts between ADAMTS13 exosites and von Willebrand factor domain A2 contribute to substrate specificity. Weiqiang Gao, Patricia J. Anderson, and J. Evan Sadler. Blood. 2008 September 1; 112(5): 1713–1719.  Click "Reactions were stopped with EDTA, and then desalted by adsorption on C18 micropipette tips (Glygen, Columbia,
MD) and elution with 60% acetonitrile/0.1% formic acid."
NuTip C18
Peptide Purification / desalting 2008 Julia Gross, Susan Grass, Alan E. Davis, Petra Gilmore-Erdmann, R. Reid Townsend and Joseph W. St. Geme III, The Haemophilus influenzae HMW1 Adhesin Is a Glycoprotein with an Unusual N-Linked Carbohydrate Modification, First Published on July 11, 2008, doi: 10.1074/jbc.M801819200 September 19, 2008 The Journal of Biological Chemistry, 283, 26010-26015. Click "All digestions were acidified to 5% formic acid, and desalted peptides were prepared using a NuTip carbon tip (Glygen) according to the manufacturer's instructions." NuTip HyperCarb
Peptide Purification / desalting 2008 Fang Shi, Ryo Yamamoto, Satoshi Shimamura, Susumu Hiraga, Norikazu Nakayama, Takuji Nakamura, Kiyoshi Yukawa, Mayumi Hachinohe, Hiroshi Matsumoto, Setsuko Komatsu, Cytosolic ascorbate peroxidase 2 (cAPX 2) is involved in the soybean response to flooding, Phytochemistry, Volume 69, Issue 6, April 2008, Pages 1295-1303. Click   NuTip C18
Peptide Purification / desalting 2007 Xin Zang and Setsuko Komatsu, A proteomics approach for identifying osmotic-stress-related proteins in rice, Phytochemistry, Volume 68, Issue 4, February 2007, Pages 426-437 Click   NuTip C18
Peptide Purification / desalting 2006 Weiqiang Gao, Patricia J. Anderson, Elaine M. Majerus, Elodee A. Tuley, and J. Evan Sadler, Exosite interactions contribute to tension-induced cleavage of von Willebrand factor by the antithrombotic ADAMTS13 metalloprotease, PNAS | December 12, 2006 | vol. 103 | no. 50 | 19099-19104. Click "Reactions were stopped with EDTA, and then desalted by adsorption on C18 micropipette tips (Glygen, Columbia,
MD) and elution with 60% acetonitrile/0.1% formic acid."
NuTip C18
Peptide Purification / desalting 2006 Tariq Mahmood , Asad Jan, Makoto Kakishima, Setsuko Komatsu, Proteomic analysis of bacterial-blight defense-responsive proteins in rice leaf blades Proteomics Volume 6, Issue 22 (2006), Pages 6053 - 6065 Click "The peptide solution thus obtained was dried and reconcen-
trated with 30 mL of 0.1% TFA in 5% ACN/water and then desalted with NuTip C-18 pipette tips (Glygen, Columbia, MD, USA)."
NuTip C18
Peptide Purification / desalting 2006 Tadashi Suzuki, Izumi Hara, Miyako Nakano, Masaki Shigeta, Takatoshi Nakagawa, Akihiro Kondo, Yoko Funakoshi and Naoyuki Taniguchi, Man2C1, an a-mannosidase is involved in the trimming of free oligosaccharides in the cytosol. Biochem. J. (2006) Click "The mixture was desalted using C18+Carbon NuTip (Hypercarb; Glygen Co., Columbia MD)." NuTip Carbon+C18
Peptide Purification / desalting 2006 Hao Wang, Jennifer E. Grant, Christopher M. Doede, Sakthivel Sadayappan, Jeffrey Robbins, Jeffery W. Walker, PKC-[beta]II sensitizes cardiac myofilaments to Ca2+ by phosphorylating troponin I on threonine-144, Journal of Molecular and Cellular Cardiology, Volume 41, Issue 5, November 2006, Pages 823-833. Click "The two samples were combined, desalted on TopTip
microcolumns (Glygen, Inc.) and analyzed by MALDI-TOF mass spectrometry"
TopTip C18
Peptide Purification / desalting 2006 Michael Wacker, Mario F. Feldman, Nico Callewaert, Michael Kowarik, Bradley R. Clarke||, Nicola L. Pohl, Marcela Hernandez, Enrique D. Vines, Miguel A. Valvano, Chris Whitfield, and Markus Aebi,Substrate specificity of bacterial oligosaccharyltransferase suggests a common transfer mechanism for the bacterial and eukaryotic systems. PNAS | May 2, 2006 | vol. 103 | no. 18 | 7088-7093 Click "Tryptic peptides extracted from in-gel-digested SDS/PAGE bands were desalted over a NuTip 10 Carbon cleanup tip (Glygen, Columbia, MD)." TopTip Hypercarb
Peptide Purification / desalting 2005 Oleg Chertov, John T Simpson, Arya Biragyn, Thomas P Conrads, Timothy D Veenstra and Robert J Fisher, Enrichment of low-molecular-weight proteins from biofluids for biomarker discoveryExpert Review of Proteomics, January 2005, Vol. 2, No. 1, Pages 139-145 . Click "TopTip™ pipet tip (Glygen Corp.) is more efficient in separating precipitate and soluble material than centrifugation." NuTip C18
Peptide Purification / desalting 2003 A.J. Alpert,A.K. Shukla, ABRF 2003 (2/2003; Denver): Poster# P111-W, Precipitation of Large, High-Abundance Proteins from Serum with Organic Solvents. Click "TopTip™disposable SPE pipet tip packed with C-18-coated silica (bed volume 25 μl) [Glygen Corp. item# TT2C18] was conditioned with two washes of 50 μl ACN." TopTip C18
Peptide Purification / desalting 1999 E.T. Chin and D.I.Papac. The Use of Porous Graphite Carbon Column for Desalting Hydropilic peptides prior to Matrix Assisted Desorption/ Ionization Time-of-Flight Mass Spectrometry Analytical Biochemistry 1999, 273, 179-185.     TopTip Hypercarb
Peptide Purification / desalting 2010 Fan-Jiang Konga, Atsushi Oyanagia and Setsuko Komatsu Cell wall proteome of wheat roots under flooding stress using gel-based and LC MS/MS-based proteomics approaches Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics
Volume 1804, Issue 1, January 2010, Pages 124-136
Click "The peptide solution was desalted with NuTip C-18 pipet tips (Glygen, Columbia, MD, USA)." NuTip C18
Peptide Purification / desalting 2010 Mohammad-Zaman Nouri, Setsuko Komatsu Comparative analysis of soybean plasma membrane proteins under osmotic stress using gel-based and LC MS/MS-based proteomics approaches PROTEOMICS Volume 10, Issue 10, pages 1930–1945, No. 10 May 2010 Click "The resulting peptides were concentrated and desalted using a NuTip C-18 pipet tips (Glygen, Columbia, MD, USA) and analyzed by nanoLC MS/MS for protein identification." NuTip C18
Peptide Purification / desalting 2010 Jian-Hua XIONG, Bin-Ying FU, Hua-Xue XU, and Yang-Sheng LI, Proteomic analysis of PEG-simulated drought stress responsive proteins of rice leaves using a pyramiding rice line at the seedling stage Botanical Studies (2010) 51: 137-145. Click "The peptide solution thus obtained was dried and reconcentrated with 30 ml of 0.1% TFA in 5% ACN/water and then desalted with NuTip C-18 pipette tips (Glygen, USA)." NuTip C18
Peptide Purification / desalting 2010 Afroz, Amber; Rashid Khan, Muhammad; Komatsu, Setsuko Determination of Proteins Induced in Response to Jasmonic Acid and Salicylic Acid in Resistant and Susceptible Cultivars of Tomato Protein and Peptide Letters, Volume 17, Number 7, July 2010 , pp. 836-846(11) Click "The resulting peptide solution was dried and reconstituted with 30 μL of 0.1% trifluoroacetic acid
in 5% acetonitrile and then desalted with NuTip C18 pipette tips (Glygen, Columbia, MD, USA)."
NuTip C18
Peptide Purification / desalting 2010 Afroz, Amber; Hashiguchi, Akiko; Khan, Muhammad R.; Komatsu, Setsuko Analyses of the Proteomes of the Leaf, Hypocotyl, and Root of Young Soybean Seedlings Protein and Peptide Letters, Volume 17, Number 3, March 2010 , pp. 319-331(13) Click "The resulting peptide solution was dried and reconstituted with 30 μL of 0.1% trifluoroacetic acid
in 5% acetonitrile and then desalted with NuTip C18 pipette tips (Glygen, Columbia, MD)"
NuTip C18
Peptide Purification / desalting 2010 Yoshiaki Furukawa, Kumi Kaneko, Koji Yamanaka and Nobuyuki Nukina Mutation-dependent polymorphism of Cu,Zn-superoxide dismutase aggregates in the familial form of amyotrophic lateral sclerosis Journal of Biological Chemistry April 19, 2010 Click "...desalted using NuTip C-18 (Glygen Co.)" NuTip C18
Peptide Purification / desalting 2009 Akiko Hashiguchi, Katsumi Sakata and Setsuko Komatsu, Proteome Analysis of Early-Stage Soybean Seedlings under Flooding Stress, J. Proteome Res., 2009, 8 (4), pp 2058–206 Click "The peptide solution obtained was dried and reconcentrated with 30 μL of 0.1% trifluioroacetic acid in 50% acetonitrile and desalted with NuTip C-18 pipet tips (Glygen, Columbia, MD)." NuTip C18
Peptide Purification / desalting 2009 Bekim Bajrami, Yu Shi, Pascal Lapierre, Xudong Yao, Shifting Unoccupied Spectral Space in Mass Spectrum of Peptide Fragment Ions, Journal of the American Society for Mass Spectrometry, Volume 20, Issue 11, November 2009, Pages 2124-2134. Click "The resulting peptides were desalted using HLB material
(Waters), which was packed in-house in TopTips (10–200 μL; Glygen, Columbia, MD"
TopTip Empty
Peptide Purification / desalting 2009 Nisar Ahmad Khan, Ryoji Takahashi, Jun Abe, Setsuko Komatsu, Identification of cleistogamy-associated proteins in flower buds of near-isogenic lines of soybean by differential proteomic analysis, Peptides, Volume 30, Issue 12, December 2009, Pages 2095-2102 (Corrected Proof - Note to users, doi:10.1016/j.peptides.2009.08.012.) Click "The peptide solution obtained was dried and
reconcentrated with 30 μL of 0.1% trifluoroacetic acid in 50% acetonitrile and desalted with NuTip C-18 pipette tips (Glygen, Columbia, MD, USA)."
NuTip C18
Peptide Purification / desalting 2009 Setsuko Komatsu, Ryo Yamamoto, Yohei Nanjo, Yoji Mikami, Harunobu Yunokawa and Katsumi Sakata , A Comprehensive Analysis of the Soybean Genes and Proteins Expressed under Flooding Stress using Transcriptome and Proteome Techniques, J. Proteome Res., 2009, 8 (10), pp 4766–4778. Click "...peptide solution obtained was dried and reconcentrated with 30 μL of 0.1% trifluioroacetic acid in 50% acetonitrile and desalted with NuTip C-18 pipet tips (Glygen, Columbia, MD)" NuTip C18
Peptide Purification / desalting 2009 Mahmood, Tariq; Kakishima, Makoto; Komatsu, Setsuko, Protein and Peptide Letters, Volume 16, Number 9, September 2009 , pp. 1041-1052(12), Proteome Analysis of Probenazole-Effect in Rice-Bacterial Blight Interactions. Click "The peptide solution, thus obtained was dried, reconcentrated and desalted with NuTip C-18 pipett tips (Glygen, Columbia, MD, USA)." NuTip C18
Peptide Purification / desalting 2008 Toshihiro Ishizawa, Yusuke Nozaki, Takuya Ueda, Nono Takeuchi, The human mitochondrial translation release factor HMRF1L is methylated in the GGQ motif by the methyltransferase HMPrmC, Biochemical and Biophysical Research Communications, Volume 373, Issue 1, 15 August 2008, Pages 99-103. Click "The peptides were further purified using a Nutip C-18 column (Glygen Corp.) before MALDI-TOF MS analysis" NuTip C18
Peptide Purification / desalting 2008 YanWen Feng , Setsuko Komatsu, Toshiko Furukawa, Tomokazu Koshiba and Yoshihisa Kohno Proteome analysis of proteins responsive to ambient and elevated ozone in rice seedlings , Agriculture, Ecosystems & Environment, Volume 125, Issues 1-4, May 2008, Pages 255-265. Click   NuTip C18
Peptide   2008 Benfang Ruan, et. al. Binding of rapamycin analogs to calcium channels and FKBP52 contributes to their neuroprotective activities, PNAS January 8, 2008 vol. 105 no. 1 33-38 Click "TopTip P-4 column was from Glygen (Columbia, MD)" TopTip P-4 (Gel filtration)
Phospho-peptide Enrichment 2010 Vishram P. Kedar, Martyn K. Darby, Jason G. Williams, and Perry J. Blackshear Phosphorylation of Human Tristetraprolin in Response to Its Interaction with the Cbl Interacting Protein CIN85 PLoS One. 2010; 5(3): e9588. Click "For phosphopeptide enrichment, TiO2 tips (Glygen) were employed using essentially the manufacturer's recommended protocol." NuTip TiO2
Phospho-peptide Enrichment 2010 Mikkat S, Lorenz P, Scharf C, Yu X, Glocker MO, Ibrahim SM. MS characterization of qualitative protein polymorphisms in the spinal cords of inbred mouse strains. Proteomics. 2010 Mar;10(5):1050-62. Click "Porous titanium dioxide immobilized to the inner surface of pipette tips (NuTipTM, Glygen, Columbia, MD, USA) was used for the selective enrichment of phosphorylated peptides. " NuTip TiO2
Phospho-peptide Enrichment 2010 John D. Leszyk Evaluation of the New MALDI Matrix 4-Chloro-α-Cyanocinnamic Acid J Biomol Tech. 2010 July; 21(2): 81–91. Click "Phosphopeptides were enriched using a modification of the published method.7 The dried peptide digest was dissolved in 10 μl binding solvent (75:19:6 acetonitrile:water:TFA). A 1- to 10-μl TiO2  NuTip was washed two times with 10 μl aliquots of binding solvent. Phosphopeptides were bound by repetitive pipetting (20 times) of the 10-μl sample volume. The NuTip was then washed five times with binding solvent and then five times with 0.1% TFA. Phosphopeptides were then eluted in a separate tube with a 10-μl vol 6% NH4OH after repetitive pipetting (10 times). A 5-μl aliquot of 5% TFA was then added, and the phosphopeptides were concentrated further and purified prior to MALDI analysis using a micro C18 ZipTip." NuTip TiO2
Phospho-peptide Enrichment 2010 Matthew B. Gatesa, Kenneth B. Tomera and Leesa J. Deterding. Comparison of Metal and Metal Oxide Media for Phosphopeptide Enrichment Prior to Mass Spectrometric Analyses. JASMS June 2010. Click "Both MALDI and ESI-MS analyses of the TiO2 NuTip eluents resulted in the reproducible observation of a greater number of unique sites of phosphorylation with the least amount of nonspecific binding compared with the other MOAC resins. ... Enrichment procedures before MS analyses dramatically improve detection and sequencing of phosphopeptides..." NuTip TiO2
Phospho-peptide Enrichment 2010 Uma K. Aryal, Andrew R. S. Ross. Enrichment and analysis of phosphopeptides under different experimental conditions using titanium dioxide affinity chromatography and mass spectrometry. Rapid Communications in Mass Spectrometry Volume 24, Issue 2, pages 219–231, 30 January 2010. Click "To address these uncertainties, we have evaluated the performance of TiO2-MOAC under different
loading and elution conditions, using a single make and model of commercially available TiO2
column (NuTipTM NT1TIO, Glygen Corp., USA) to minimize experimental variability."
NuTip TiO2
Phospho-peptide Enrichment 2010 Jamie D. Dunn, Gavin E. Reid, Merlin L. Bruening Techniques for phosphopeptide enrichment prior to analysis by mass spectrometry Mass Spectrometry Reviews, Volume 29, Issue 1, pages 29–54, January 2010 Click "...Microtips that contained zirconium dioxide (Glygen, Columbia, MD) were utilized for the enrichment of phosphorylated peptides, and the specificity and recovery achieved was compared to that obtained with Glygen microtips containing titanium dioxide (with the same binding, rinsing and elution solutions)... Overall, the TiO2 microtips were more selective for enrichment of multiply phosphorylated peptides, whereas the ZrO2 tips enriched primarily monophosphorylated peptides. " NuTip and TopTip TiO2
Phospho-peptide Enrichment 2010 Parvathi Rudrabhatla, Philip Grant, Howard Jaffe, Michael J. Strong,  and Harish C. Pant Quantitative phosphoproteomic analysis of neuronal intermediate filament proteins (NF-M/H) in Alzheimer’s disease by iTRAQ FASEB J..2010; 0: fj.10-157859v1 Click "...phopeptide enrichment by TiO2 chromatography utilizing a 1- to 200-l TiO2 Top
Tip (Glygen Corp., Columbia, MD, USA) essentially according to the method of Wu
et al. "
TopTip TiO2
Phospho-peptide Enrichment 2010 Özlü, Nurhan and Monigatti, Flavio and Renard, Bernhard Y. and Field, Christine M. and Steen, Hanno and Mitchison, Timothy J. and Steen, Judith J. Binding Partner Switching on Microtubules and Aurora-B in the Mitosis to Cytokinesis Transition Mol Cell Proteomics 2010 9: 336-350. First Published on September 28, 2009, doi:10.1074/mcp.M900308-MCP200 Click "TiO 2 was used to enrich for phosphopeptides. Briefly, lyophilized samples were dissolved
in binding buffer (5% TFA, 40% acetonitrile). After loading samples onto the TiO 2 columns
(TopTip, Glygen), the column was washed with the binding buffer."
TopTip TiO2
Phospho-peptide Enrichment 2010 Weitao Jia, Justin F. Shaffer, Samantha P. Harris, Julie A. Leary Identification of Novel Protein Kinase A Phosphorylation Sites in the M-domain of Human and Murine Cardiac Myosin Binding Protein-C Using Mass Spectrometry Analysis Journal of Proteome Research  2010  9  (4), 1843-1853 Click "The phosphopeptides were also enriched using TopTips filled with TiO 2 (particle size 20−30 μm, Glygen Corp., Columbia, MD) as described in the manufacturer's instruction with minor modifications." TopTip TiO2
Phospho-peptide Enrichment 2010 Michael Mazanek, Elisabeth Roitinger, Otto Hudecz, James R.A. Hutchins, Bjorn Hegemann, Goran Mitulovic, Thomas Taus, Christoph Stingl, Jan-Michael Peters, Karl Mechtler, A new acid mix enhances phosphopeptide enrichment on titanium- and zirconium dioxide for mapping of phosphorylation sites on protein complexes, Journal of Chromatography B, Volume 878, Issues 5-6, 15 February 2010, Pages 515-524. Click     TiO2/ZrO2
Phospho-peptide Enrichment 2009 R. Luke Wiseman, King-Tung Chin, Cole M. Haynes1 Ariel Stanhill, Chong-FengXu, Assen Roguev, Nevan J. Krogan, Thomas A. Neubert and David Ron: hioredoxin related protein 32 is an arsenite-regulated thiol reductase of the proteasome 19S particle, JBC Papers in Press. Published on April 6, 2009 Click "For the phospho-SILAC experiments, the extracted
peptides were further purified on a TiO 2 tip (1-10-μl NuTip; Glygen)."
NuTip Carbon
Phospho-peptide Enrichment 2009 MVS Sivaram, TL Wadzinski, SD Redick, T Manna, SJ: Dynein light intermediate chain 1 is required for progress through the spindle assembly chckpoint. The EMBO Journal, 2009 – natue.com. Click "Phosphorylated tryptic peptides were enriched for using NuTip TiO2 micropipette tips (Glygen Corporation) using a modified protocol" NuTip TIO2
Phospho-peptide Enrichment 2009 Mingquan Guo: A phosphoproteomic strategy compatible with systems biology analysis, doi:10.1016/j.ab.2009.04.008 Anal. Biochem. Click "TiO 2 particles (50 μm) were purchased from Glygen (Columbia, MD, USA)." Media TiO2
Phospho-peptide Enrichment 2009 R. Luke Wiseman, King-Tung Chin, Coe M. Haynes, Ariel Stanhill, Chong-Feng Xu, Assen Roguev, Nevan J. Krogan, Thomas A. Neubert, and David Ron: Thioredoxin-related Protein 32 Is an Arsenite-regulated Thiol Reductase of the Proteasome 19 S Particle J. Biol. Chem., Vol. 284, Issue 22, 15233-15245, May 29, 2009 Click "For the phospho-SILAC experiments, the extracted
peptides were further purified on a TiO 2 tip (1-10-μl NuTip; Glygen)."
NuTip TIO2
Phospho-peptide Enrichment 2009 Anne M. Distler, Janos Kerner, Kwangwon Lee, Charles L. Hoppel, Chapter 6 Modifications of Mitochondrial Outer Membrane Proteins, In: William S. Allison and Anne N. Murphy, Editor(s), Methods in Enzymology, Academic Press, 2009, Volume 457, Mitochondrial Function, Part B: Mitochondrial Protein Kinases, Protein Phosphatases and Mitochondrial Diseases, Pages 97-115. Click "The porous titanium and zirconium oxide microtips are
purchased from Glygen (Columbia, MD)."
NuTip TiO2/ZrO2
Phospho-peptide Enrichment 2009 Mingquan Guo, A phosphoproteomic strategy compatible with systems biology analysis, Analytical Biochemistry, In Press, Corrected Proof, Available online 11 April 2009. Click "TiO 2 particles (50 μm) were purchased from Glygen (Columbia, MD, USA)." Media TiO2
Phospho-peptide Enrichment 2009 Ryan Chong, Rachel Swiss, Gabriel Briones, Kathryn L. Stone, Erol E. Gulcicek, Herve Agaisse, Regulatory Mimicry in Listeria monocytogenes Actin-Based Motility, Cell Host & Microbe, Volume 6, Issue 3, 17 September 2009, Pages 268-278. Click   NuTip TiO2
Phospho-peptide Enrichment 2009 Jesse Rinehart, Yelena D. Maksimova, Jessica E. Tanis, Kathryn L. Stone, Caleb A. Hodson, Junhui Zhang, Mary Risinger, Weijun Pan, Dianqing Wu, Christopher M. Colangelo, Biff Forbush, Clinton H. Joiner, Erol E. Gulcicek, Patrick G. Gallagher, Richard P. Lifton, Sites of Regulated Phosphorylation that Control K-Cl Cotransporter Activity, Cell, Volume 138, Issue 3, 7 August 2009, Pages 525-536. Click "Peptides were extracted with 0.5% trifluoroacetic acid, dried, resuspended, and applied to a TiO2 TopTip micro-spin column (Glygen Corp.)." TopTip TiO2
Phospho-peptide Enrichment 2009 Zenglan Wang, Gaofeng Dong, Sasha Singh, Hanno Steen, Jiaxu Li, A simple and effective method for detecting phosphopeptides for phosphoproteomic analysis, Journal of Proteomics, Volume 72, Issue 5, Special Section: "From Genome to Proteome: Integration and proteome completion", 21 July 2009, Pages 831-835. Click "The peptide solution was then added to 2.6 mg of equilibrated TiO 2 beads (Glygen) in a microspin column…" Media TiO2
Phospho-peptide Enrichment 2009 Chitra Rajagopal, Kathryn L. Stone, Victor P. Francone, Richard E. Mains and Betty A. Eipper , Secretory Granule to the Nucleus, ROLE OF A MULTIPLY PHOSPHORYLATED INTRINSICALLY UNSTRUCTURED DOMAIN, First Published on July 27, 2009, doi: 10.1074/jbc.M109.035782 September 18, 2009 The Journal of Biological Chemistry, 284, 25723-25734. Click " TiO 2 Top Tips (Glygen Corp.) were washed with
40 μl of 100% acetonitrile, followed by 0.2 m sodium phosphate, pH 7.0, and then by 0.5% trifluoroacetic acid, 50% acetonitrile."
TopTip TiO2
Phospho-peptide Enrichment 2009 Emily S. Boja, Darci Phillips, Stephanie A. French, Robert A. Harris and Robert S. Balaban, Quantitative Mitochondrial Phosphoproteomics Using iTRAQ on an LTQ-Orbitrap with High Energy Collision Dissociation, J. Proteome Res., 2009, 8 (10), pp 4665–4675. Click "Titanium dioxide prepacked toptips were obtained from Glygen (Columbia, MD)." TopTip TiO2
Phospho-peptide Enrichment 2009 Minsoo Oh, Hangun Kim, Ilhwan Yang, Ja-Hye Park, Wei-Tao Cong, Moon-Chang Baek, Sonja Bareiss, Hyunkyoung Ki, Qun Lu, Jinhyung No, Inho Kwon, Jung-Kap Choi and Kwonseop Kim, GSK-3 phosphorylates delta-catenin and negatively regulates its stability via ubiquitination/proteosome-mediated proteolysis, First Published on August 25, 2009, doi: 10.1074/jbc.M109.002659. Click "After trypsin digestion, phosphopeptides were enriched using
phosphopeptide enrichment… TiO 2 (Glygen Corp.) according to the manufacturer's protocol."
TopTip TiO2
Phospho-peptide Enrichment 2008 Jamie D. Dunn, Elizabeth A. Igrisan, Amanda M. Palumbo, Gavin E. Reidand Merlin L. Bruening: Phosphopeptide Enrichment Using MALDI Plates Modified with High-Capacity Polymer Brushes, Anal. Chem., 2008, 80 (15), pp 5727–5735 Click "Pipet tips containing 30 μg of TiO 2 or ZrO 2 embedded into the walls of the tip were purchased from Glygen. These systems have a binding capacity of 1−2 μg of peptide." NuTip TiO2/ZrO2
Phospho-peptide Enrichment 2008 Chao Yuan, Quanhu Sheng,Haixu Tang, Yixue Li, Rong Zeng, and R. John Solaro, Quantitative comparison of sarcomeric phosphoproteomes of neonatal and adult rat hearts, Am J Physiol Heart Circ Physiol 295: H647-H656, 2008. Click "TiO 2 Nutips (Glygen, Columbia, MD) were washed with binding buffer (10% ACN-2% FA), then with 10 mg/ml dihydroxybenzoic (DHB; Sigma-Aldrich)…" NuTip TIO2
Phospho-peptide Enrichment 2008 Marit Lenman, Carolin Sörensson, and Erik Andreasson Enrichment of Phosphoproteins and Phosphopeptide Derivatization Identify Universal Stress Proteins in Elicitor-Treated Arabidopsis. Molecular Plant-Microbe Interactions, October 2008, Volume 21, Number 10, Pages 1275-1284. Click "Prepacked TiO2 Nutip columns from Glygen…" NuTip TiO2
Phospho-peptide Enrichment 2008 Matthias Rainer , Harald Sonderegger , Rania Bakry , Christian W. Huck , Sandra Morandell , Lukas A. Huber , Douglas T. Gjerde , Günther K. Bonn, Analysis of protein phosphorylation by monolithic extraction columns based on poly(divinylbenzene) containing embedded titanium dioxide and zirconium dioxide nano-powders. Proteomics Volume 8 Issue 21 (2008),Pages4593–4602 Click   TopTip TiO2
Phospho-peptide Enrichment 2008 Scott B. Ficarro, Jignesh R. Parikh, Nathaniel C. Blankand Jarrod A. Marto: Niobium(V) Oxide (Nb2O5): Application to Phosphoproteomics. Anal. Chem., 2008, 80 (12), pp 4606–4613 Click "MALDI mass spectra of tryptic α-casein peptides enriched by
TiO 2 and a mixed bed of TiO 2 and Nb 2 O 5 , each in TopTip format."
TopTip TiO2
Phospho-peptide Enrichment 2008 Matthew B. Gates, KennethB.Tomer and LeesaJ.Deterding: MALDI/MS Comparison of Fe-NTA Immobilized Metal Affinity Chromatography and Commercially-Available Metal Oxide Affinity Resins for Phosphopeptide Enrichment, NATO Science for Peace and Security Series A: Chemistry and Biology, Applications of Mass Spectrometry in Life Safety, p. 37-54 (2008). Click "Six different resins are compared: Glygen TiO2 NuTip, ZrO2 NuTip, mixed ZrO2 and TiO2 NuTip,Titansphere TiO2, PhosTrap magnetic titanium beads, and a Fe-NTA resin. " NuTip and TopTip TiO2/ZrO2
Phospho-peptide Enrichment 2008 Eric S. Simon, Matthew Young, Antonia Chana, Zhao-Qin Bao and Philip C. Andrews, Improved enrichment strategies for phosphorylated peptides on titanium dioxide using methyl esterification and pH gradient elution, Analytical Biochemistry, Volume 377, Issue 2, 15 June 2008, Pages 234-242. Click "TiO 2 particles (50 μm) were purchased from Glygen (Columbia, MD)" Media TiO2
Phospho-peptide Enrichment 2008 Mika Teranishi, Kentaro Nakamura, Hiroshi Morioka, Kazuo Yamamoto and Jun Hidema: The Native Cyclobutane Pyrimidine Dimer Photolyase of Rice Is Phosphorylated, Plant Physiology 146:1941-1951 (2008). Click "recover the phosphorylated peptides from tryptic peptide mixtures of the 56-kD CPD photolyase using a titanium dioxide microcolumn (Glygen)" TopTip TiO2
Phospho-peptide Enrichment 2008 Adam J. Carroll, Joshua L. Heazlewood, Jun Ito, A. Harvey Millar, Analysis of the Arabidopsis cytosolic ribosome proteome provides detailed insights into its components and their post-translational modification, Journal of Biological Chemistry, 2008 ASBMB, ... 283, Issue 9, 5801-5814, February 29, 2008. Click "Phosphopeptide Enrichment Using Titanium Dioxide—. TiO 2 tips (NuTip) were supplied by Glygen Inc., and phosphopeptide enrichment procedures were essentially those outlined by Larsen et al. (24) with some modifications." NuTip TiO2
Phospho-peptide Enrichment 2008 M Mazanek, G Mitulovi, F Herzog, C Stingl, JRA Titanium dioxide as a chemo-affinity solid phase in offline phosphopeptide chromatography prior to 11 enriched phosphopeptides by using self-packed TiO 2 tips, 2008. Click "enriched phosphopeptides by using self-packed TiO 2 tips, but we decided to use commercially available pre-packed "TopTips" from Glygen" TopTip TiO2
Phospho-peptide Enrichment 2008 Liwen Wang; John C. Byrd; Michael A. Freitas and Raj Muthusamy. A Tandem Phosphoprotein / Phosphopeptide Enrichment Strategy for the Characterization of Signaling Proteins in Chronic Lymphocytic Leukemia.Presentation, Fifth Annual Ohio Mass Spectrometry Symposium March 24-25, 2008 Click "Phosphopeptides were then enriched by use of zirconium dioxide coated NuTip (Glygen Corp, Columbia, MD)." NuTip ZrO2
Phospho-peptide Enrichment 2008 Brian Roberts, Jan Pohl and Jennifer L. Gooch: A fluorimetric method for determination of calcineurin activity, Cell Calcium, Volume 43, Issue 5, May 2008, Pages 515-519. Click "Titanium-oxide (TiO 4 )-coated plates were obtained from Glygen (Colombus, MA)" Lab in a Plate TiO2
Phospho-peptide Enrichment 2007 Henrik Molina, David M. Horn, Ning Tang, Suresh Mathivanan, and Akhilesh Pandey: Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry, PNAS February 13, 2007 vol. 104 no. 7 2199-2204. Click "The peptides obtained from the digestion step were dried down, and selected fractions were redissolved in 20 μl of 80% acetonitrile, 66 mg/ml 2,5-dihydrobenzoic acid, and 1% TFA and loaded onto a home-built TiO 2 (Glygen Corp, Columbia MD) microcolumn" NuTip TiO2
Phospho-peptide Enrichment 2007 Eugen Damoc, Christopher S. Fraser, Min Zhou, Hortense Videler, Greg L. Mayeur, John W. B. Hershey, Jennifer A. Doudn, Carol V. Robinson, and Julie A. Leary : Structural Characterization of the Human Eukaryotic Initiation Factor 3 Protein Complex by Mass Spectrometry. Molecular & Cellular Proteomics 6:1135-1146, 2007. Click "Microtips filled with TiO2 were purchased from Glygen and used according to the manufacturer's instructions." NuTip TiO2
Phospho-peptide Enrichment 2007 Xin-De Zheng, Raymond Teck Ho Lee, Yan-Ming Wang, Qi-Shan Lin and Yue Wang The EMBO Journal advance online publication 2 August 2007, Phosphorylation of Rga2, a Cdc42 GAP, by CDK/Hgc1 is crucial for Candida albicans hyphal growth. doi: 10.1038/sj.emboj.7601814, The EMBO Journal (2007), 1–10 Click "The phosphopeptides were enriched using TiO2 TopTip (Glygen Inc., Columbia, NY). The bound peptides were eluted with 0.5% NH3OH." TopTip TiO2
Phospho-peptide Enrichment 2007 D. A. Moraga, M. C. Chow, I. Isaac, and S. M. Stevens, P77-M Automated Analysis of Gel-Derived Phosphoproteins Using the Investigator Proteomic System, Jr. J Biomol Tech. 2007 February; 18(1): 26. Click "Tryptic digests from each protein spot were processed with the ProMS workstation using TiO2  microcolumns (Glygen Corp., Columbia, MD) for phosphopeptide enrichment prior to MALDI-TOF/TOF analysis (ABI 4700 Proteomics Analyzer)." TopTip TiO2
Phospho-peptide Enrichment 2007 Liwen Wang, Hua Xu, Chen Ren, Shujun Liu, Guido Marcucci and Michael A. Freitas (Use of ZrO2 Tips)Enrichment and Characterization of Histone H1 Phosphorylation Isoforms in Chemoprevention of Acute Myeloid Leukemia. 4th Annual Ohio Mass Spectrometry Symposium (March 19-20 2007) 3rd Annual     NuTip ZrO2
Phospho-peptide Enrichment 2007 Eugen Damoc, Christopher S. Fraser, Min Zhou, Hortense Videler, Greg L. MayeurJohn W. B. Hershey, Jennifer A. Doudna, Carol V. Robinson and Julie A. Leary: Structural Characterization of the Human Eukaryotic Initiation Factor 3. Click "Microtips filled with TiO 2 were purchased from Glygen and used according to the manufacturer's instructions." NuTip TiO2
Phospho-peptide Enrichment 2007 DJ Jang, M Guo, D Wang. Proteomic and biochemical studies of calcium-and phosphorylation-dependent calmodulin complexes in mammalian cells. J. Proteome Res., 2007, 6 (9), pp 3718–3728 Click "Aliquots of the tryptic digests were enriched for phosphopeptides using TiO 2 columns
(Glygen Inc.) as described 23 and analyzed by LC−MS/MS"
  TiO2
Phospho-peptide Enrichment 2006 Christopher J. Toher ; Adam W. Perala; Carla J. Marshall-Waggett; Gary A. Valaskovic; Ashok K. Shukla; A.A. Oetting; M.M. Shukla; N. Manohar. Online and Offline Nanoelectrospray Analysis of Phosphopeptides Purified by TiO2, ZrO2, and Carbon Wall-Coated Pipette Tips. ASMS 2006 Poster TP 215     NuTip Carbon
Phospho-peptide Enrichment 2006 H. K. Kweon and K. Hakansson. Selective zirconium dioxide-based enrichment of phosphorylated peptides for mass spectrometric analysis. Anal Chem. 2006 Mar 15;78(6):1743-9. Click "Microtips filled with ZrO2 and TiO2 (25 or 50 μg) ...from Glygen (Columbia, MD) [were] used without further modification." NuTip TiO2/ZrO2
Phospho-peptide Enrichment 2006 Michael Mazanek, Goran Mituloviae, Franz Herzog, Christoph Stingl, James RA Hutchins, Jan Michael Peters and Karl Mechtler Titanium dioxide as a chemo-affinity solid phase in offline phosphopeptide chromatography prior to HPLC-MS/MS analysis Click "TiO2 -filled tips: TopTips, produced by Glygen" TopTIp TiO2
Phospho-peptide Enrichment 2006 Christopher J. Toher, Adam W. Perala, Ashok K. Shukla, Gary A. Valaskovic Offline Nano-ESI Phosphopeptide Analysis with Carbon, TiO2, and ZrO2, wall-Coated Trap'nTips™, ABRF 2006     TopTip TiO2
Phospho-peptide Enrichment 2006 King JB, Gross J, Lovly CM, Rohrs H, Piwnica-Worms H, Townsend RR. Accurate mass-driven analysis for the characterization of protein phosphorylation. Study of the human Chk2 protein kinase.Anal Chem. 2006 Apr 1; 78(7):2171-81. Click      
Phospho-peptide Enrichment 2006 Christopher J. Toher ; Adam W. Perala; Carla J. Marshall-Waggett; Gary A. Valaskovic; Ashok K. Shukla; A.A. Oetting; M.M. Shukla; N. Manohar. Online and Offline Nanoelectrospray Analysis of Phosphopeptides Purified by TiO2, ZrO2, and Carbon Wall-Coated Pipette Tips. ASMS 2006 Poster TP 215 ( for Hypercarb and TiO2)     NuTip and TopTip POROS RP2
Phospho-peptide Enrichment 2006 Maja Matis; Urs A. Meyer. Phosphoproteome analysis of primary human hepatocytes: effect of exposure to inducers of cytochromes P450. ASMS-2006 POSTER No. Th 658.     NuTip and TopTip POROS RP2
Phospho-peptide Enrichment 2006 Maja Matis, Ragna Sack and Urs A. Meyer. Role of protein phosphorylation/dephosphorylations in the regulation of cytochrome P450 genes ASMS 2006 POSTER No. 2666     NuTip and TopTip POROS RP2
Phospho-peptide Enrichment 2006 Qishan Lin, Jinghua Zhu. Application Titanium Dioxide IMAC for Enrichment Phosphopeptides Prior to Tandem Mass Spectrometry., U Albany Center for Functional Genomics Click   NuTip and TopTip POROS RP2
Phospho-peptide Enrichment 2006 Julie B. King, Julia Gross, Christine M. Lovly, Henry Rohrs,Helen Piwnica-Worms, and R. Reid Townsend, Accurate Mass-Driven Analysis for the Characterization of Protein Phosphorylation. Study of the Human Chk2 Protein Kinase, Anal. Chem., 2006, 78 (7), pp 2171–2181. Click      
Phospho-peptide Enrichment 2006 Christopher J. Toher, Adam W. Perala, Ashok K. Shukla, Gary A. Valaskovic:  Offline Nano-ESI Phosphopeptide Analysis with TiO2, and ZrO2 Wall-Coated Trap'nTips™, ASMS 2006        
Phospho-peptide Enrichment 2004 Nelli I. Taranenko, Vladimir M. Doroshenko, Ashok K. Shukla, and Mukta M. Shukla. Applications of AP-MALDI Ion Trap Mass Spectrometry for the Analysis of Phosphopeptides, AMERICAN LABORATORY 36 MAY 2004 Click   NuTip IMAC
Phospho-peptide Enrichment 2004 V. M. Doroshenko, N. I. Taranenko, A. K. Shukla, M. M. Shukla Phospho- and Glyco-peptides Analysis Using Negative and Positive AP-MALDI Ion Trap Mass Spectrometry. ABRF2004        
Phospho-peptide Enrichment 2004 V. M. Doroshenko, N. I. Taranenko, A. K. Shukla, M. M. Shukla AP-MALDI Ion Trap Mass Spectrometry Analysis of Selective Phosphopeptides Using Different Immobilized Metal Affinity Chromatography Materials (ASMS 2004)        
Phospho-peptide Enrichment 2002 Loughrey Chen S, Huddleston MJ, Shou W, Deshaies RJ, Annan RS, Carr SA. Mass spectrometry-based methods for phosphorylation site mapping of hyperphosphorylated proteins applied to Net1, a regulator of exit from mitosis in yeast. Mol Cell Proteomics. 2002 Mar;1(3):186-96.     TopTip Hypercarb
Phospho-peptide Enrichment 1999 Jenny Albanese, Senior Field Applications Specialist ABI- Proteomics Phospho workshop at Mississippi State University: IMAC in combination with LC-Maldi enables femtomole detection of phosphopeptides. (1999) Profiling the Phosphoproteome of Endometrial Carcinoma.     NuTip and TopTip POROS RP2
Phospho-peptide Purification / desalting 2007 Andrew J. Alpert; Steven P. Gygi; Ashok K. Shukla, Desalting Phosphopeptides by Solid-Phase Extraction (POSTER ASMS 2007)        
Phospho-peptide Purification / desalting 2006 Adrian M. Taylor; Leroi DeSouza; K.W. Michael Siu Profiling the Phosphoproteome of Endometrial Carcinoma ASMS 2006. Poster No. TP462. Click   TopTip C18
Phospho-protein Enrichment 2008 Scott B. Ficarro, Jignesh R. Parikh, Nathaniel C. Blank and Jarrod A. Marto, Niobium(V) Oxide (Nb2O5): Application to Phosphoproteomics,Anal. Chem., 2008, 80 (12), pp 4606–4613        
Glyco-peptide Enrichment 2010 Susan Grass, Cheryl F. Lichti, R. Reid Townsend, Julia Gross, and Joseph W. St. Geme, III The Haemophilus influenzae HMW1C Protein Is a Glycosyltransferase That Transfers Hexose Residues to Asparagine Sites in the HMW1 Adhesin PLoS Pathog. 2010 May; 6(5): e1000919. Click "Peptides were acidified with 5.5 цl formic acid (Sigma) and extracted 6 times with 10–200 цl NuTip
porous graphite carbon wedge tips (Glygen) according to the manufacturer's directions and were
then eluted into 1.5 ml autosampler vials with 60% acetonitrile"
NuTip HyperCarb
Glyco-peptide Enrichment 2008 Kazutosi Kubota, Yuji Sato, Yusuke Suzuki, Naoko Goto-Inoue, Tosifusa Toda, Minoru Suzuki, Shin-ichi Hisanaga, Akemi Suzuki and Tamao Endo, Analysis of Glycopeptides Using Lectin Affinity Chromatography with MALDI-TOF Mass Spectrometry, Anal. Chem., 2008, 80 (10), pp 3693–3698 Click "Top Tips were purchased from Glygen Corp. (Columbia, MD)." TopTip Lectin
Glyco-peptide Enrichment 2007 Carol L. Nilsson, Lectins: Analytical Tools from Nature, In: Carol L. Nilsson, Editor(s), Lectins, Elsevier Science B.V., Amsterdam, 2007, Pages 1-13. Click   TopTip Lectin
Glyco-peptide Enrichment 2006 Vivianne I. Otto , Eugen Damoc, Leah N. Cueni, Thomas Schurpf, Renate Frei , Sarah Ali , Nico Callewaert , Adrian Moise, Julie A. Leary, Gerd Folkers, and Michael Przybylski: N-Glycan Structures and N-Glycosylation Sites of Mouse Soluble Intercellular Adhesion Molecule-1 Revealed by MALDI-TOF and FTICR Mass Spectrometry Glycobiology Advance Access published online on July 28, 2006 Glycobiology, doi:10.1093/glycob/cwl032 Click "NuTip graphitized carbon 10-µL pipette tips were from Glygen (Columbia, MD)." NuTip Carbon
Glyco-peptide Enrichment 2006 Joseph P. Connolly, Dr. , Diego Comerci , Timothy G. Alefantis , Alexander Walz , Marian Quan , Ryan Chafin, Paul Grewal Cesar V. Mujer, Rodolfo A. Ugalde, Vito G. DelVecchio. Proteomic analysis of Brucella abortus cell envelope and identification of immunogenic candidate proteins for vaccine development. PROTEOMICS Volume 6, Issue 13 (2006) , Pages 3767 – 3780 Click "was resuspended in binding solution (15 mM ammonium acetate, pH 3.5, with 85% ACN) and loaded onto a hydrophilic interaction TopTip HILIC column" TopTip HILIC
Glyco-peptide Enrichment 2006 T Adamson, K Hakansson, Infrared Multiphoton Dissociation and Electron Capture Dissociation of High Mannose-Type Glycopeptides". J. Proteome Res. 2006, 5, 493. Click "For the overnight trypsin digest, concanavalin A tips (Glygen Corporation, Columbia, MD) were used to selectively bind glycopeptides." TopTip Lectin
Glyco-peptide Enrichment 2006 Wouter Laroy, Roland Contreras and Nico Callewaert. Glycome mapping on DNA, sequencing equipment. Nature Protocols 1, 397 - 405 (2006) Click   NuTip Carbon
Glyco-peptide Enrichment 2004 Ashok K. Shukla, Mukta M. Shukla, Vladimir M. Doroshenko, Nelli I. Taranenko. Applications of Glycosidase in Glycoproteins Structure Determination by AP-MALDI MS/MS Analysis. ASMS 2004        
Glyco-peptide Purification 2009 Eric D. Dodds, Richard R. Seipert, Brian H. Clowers, J. Bruce Germanand Carlito B. Lebrilla, Analytical Performance of Immobilized Pronase for Glycopeptide Footprinting and Implications for Surpassing Reductionist Glycoproteomics, J. Proteome Res., 2009, 8 (2), pp 502–512 Click "To compensate for the increased concentration of buffer salts, the digests were then purified by solid-phase extraction using 10 μL of Glygen NuTips loaded with graphitic carbon (Columbia, MD)." NuTip Carbon
Glyco-peptide Purification 2004 N. I. Taranenko, A. K. Shukla, M. M. Shukla, V. M. Doroshenko.  Effects of Lectins Affinity Chromatography on Glycoproteins Enrichment Using AP-MALDI Ion Trap Mass Spectrometry (2004) Click "After digestion, an aliquot of digested peptides and glycopeptides was applied to 10 µl immobilized lectin columns (TopTip)." TopTip Lectin
Glyco-peptide Purification / desalting 2006 Takashiba M, Chiba Y, Jigami Y. Identification of phosphorylation sites in N-linked glycans by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Anal Chem. 2006 Jul 15;78(14):5208-13. Click "After HPLC separation, the phosphorylated glycans were purified using ion-exchange resin Muromac and applied to porous graphitic carbon (PGC) cartridges (Nutip Carbon and GL-Pak Carbograph) for desalting" NuTip Carbon
Glyco-peptide Purification / desalting 2006 Kameyama A, Nakaya S, Ito H, Kikuchi N, Angata T, Nakamura M, Ishida HK, Narimatsu H, Strategy for simulation of CID spectra of N-linked oligosaccharides toward glycomics. J Proteome Res. 2006 Apr; 5(4):808-14. Click "The filtrates were desalted by using a NuTip-Hypercarbon (Glygen Corp., Columbia, MD) according to the manufacturer's manual." NuTip Hypercarb
Glyco-peptide Purification / desalting 2006 Leah Cueni. A method for the qualitative and quantitative analysis of the glycosylation pattern of mouse soluble intercellular adhesion molecule-1 (sICAM-1), Institute of Pharmaceutical Sciences (Diploma Thesis), 2006 Click "Samples were desalted prior to mass spectrometric analyses using NuTipTM graphitized car-bon 10μl pipette tips (Glygen Corp., Columbia, MD). Tips were conditioned by pipetting five times 10μl of 60% acetonitrile containing 0.05% trifluoroacetic acid (TFA) followed by three times 10μl of water." NuTip Carbon
Glyco-peptide Purification / desalting 2009 Afucosylated antibodies against CCR5 and their use for the treatment of inflammatory conditions, United States Patent Application 20090226434. Click "Subsequently, the glycan structures released were isolated and desalted using NuTip-Carbon pipette tips (obtained from Glygen)." NuTip HyperCarb
Glyco-peptide Purification / desalting 2008 Tadashi Suzuki, Ichiro Matsuo, Kiichiro Totani, Sho Funayama, Junichi Seino, Naoyuki Taniguchi, Yukishige Ito, Sumihiro Hase, Dual-gradient high-performance liquid chromatography for identification of cytosolic high-mannose-type free glycans, Analytical Biochemistry, Volume 381, Issue 2, 15 October 2008, Pages 224-232. Click "Fractions were desalted using C18 + Carbon NuTip (Hypercarb, Glygen, Columbia, MD)" NuTip Carbon
Glyco-peptide Purification / desalting 2008 Julia Gross, Susan Grass, Alan E. Davis, Petra Gilmore-Erdmann, R. Reid Townsend, Joseph W. St. Geme, THE HAEMOPHILUS INFLUENZAE HMW1 ADHESIN IS A GLYCOPROTEIN WITH AN UNUSUAL N-LINKED CARBOHYDRATE MODIFICATION, JBC Papers in Press. Published on July 11, 2008 Click "All digestions were acidified to 5% formic acid, and desalted peptides were prepared using a NuTip carbon tip (Glygen) according to the manufacturer's instructions." NuTip Carbon
Glyco-peptide Purification / desalting 2008 Christoph Kannicht, Detlef Grunow and Lothar Lucka, Enzymatic Sequence Analysis of N -Glycans by Exoglycosidase Cleavage and Mass Spectrometry detection of Lewis X Structures, Methods in Molecular Biology, Volume 446, Post-translational Modifications of Proteins, Humana Press, p. 255-266, (2008) Click "TopTip carbon spin column, type P2-Carbon (Glygen Corp., Columbia, USA) for sample purification" NuTip Carbon
Glyco-peptide Purification / desalting 2008 Adam W. Lucka, Bruce R. Kilgore, Rekha Patel, Bruce A. Andrien Jr. and Shirish T. Dhume, Mass Spectrometry and HPLC with Fluorescent Detection-Based Orthogonal Approaches to Characterize N -Linked Oligosaccharides of Recombinant Monoclonal Antibodies, Methods in Molecular Biology, Volume 446, Post-translational Modifi cations of Proteins, Humana Press, p. 347-36 (2008) Click "Hypercarb TopTip (Glygen Corp) (available in 3 binding-capacity levels)" NuTip Carbon
Glyco-peptide Purification / desalting 2008 Rapha Carapito, Christine Carapito, Jean-Marc Jeltsch and Vincent Phalip,Efficient hydrolysis of hemicellulose by a Fusarium graminearum xylanase blend produced at high levels in Escherichia coli., Bioresource Technology, Article in Press, Corrected Proof doi:10.1016/j.biortech. 2008. Click "…Polysaccharideswere desalted using a NuTip type P20 carbon solid-phase extraction pipette tip (Glygen, USA)." NuTip Carbon
Glyco-protein Enrichment 2010 Mizuho INAGAKI, Shuuichi NAKAYA, Daisuke NOHARA, Tomio YABE, Yoshihiro KANAMARU and Tohru SUZUKI, “The Multiplicity of N-Glycan Structures of Bovine Milk 18 kDa Lactophorin (Milk GlyCAM-1)”, Biosci. Biotechnol. Biochem., Vol. 74, 447-450 (2010) . Click "The PA-derivatized glycans were then dried with a centrifugal evaporator and dissolved
in 10 цl of 0.1% v/v trifluoroacetic acid, followed by purified via Nutip Carbon (Glygen,
Columbia, MD) according to the manufacturer's protocol."
NuTip HyperCarb
Glyco-protein Enrichment 2010  John W. Froehlich, Eric D. Dodds, Mariana Barboza, Erica L. McJimpsey, Richard R. Seipert, Jimi Francis, Hyun Joo An, Samara Freeman, J. Bruce German, Carlito B. Lebrilla Glycoprotein Expression in Human Milk during Lactation  Journal of Agricultural and Food Chemistry 2010 58 (10), 6440-6448 Click "Porous graphitized carbon cartridges were obtained from Glygen. " NuTip HyperCarb
Glyco-protein Enrichment 2010 Ming Lei, et al, 'Sequential Enrichment of Sulfated Glycans by Strong Anion-Exchange Chromatography Prior to Mass Spectrometric Measurements,' J Am Soc Mass Spectrom, 2010, 21, 348-357 Click "Sequential enrichment of sulfated glycans is attained using spin-columns packed
with strong anion exchanger"
TopTip SAX
Glyco-protein Enrichment 2008 DS Spellman, TA Neubert - Analysis of Posttranslational Modifications, Proteomics of the Nervous System, 2008 – books.google.com     NuTip and TopTip POROS RP2
Glyco-protein Enrichment 2008 JT Adamson Structural Characterization of Protein Glycosylation Utilizing Fragmentation from Gas Phase Ion- , - 2008 -, ... A dissertation submitted in partial fulfillment of the requirements for the degree, of Doctor of Philosophy (Chemistry) in The University of Michigan 2008.     NuTip and TopTip POROS RP2
Glyco-protein Enrichment 2006 An HJ, Miyamoto S, Lancaster KS, Kirmiz C, Li B, Lam KS, Leiserowitz GS, Lebrilla CB. Related Articles, Profiling of glycans in serum for the discovery of potential biomarkers for ovarian cancer. J Proteome Res. 2006 Jul;5(7):1626-35. Click   TopTip Hypercarb
Glyco-protein Enrichment 2004 N. I. Taranenko, A. K. Shukla, M. M. Shukla, V. M. Doroshenko Effects of Lectins Affinity Chromatography on Glycoproteins Enrichment Using AP-MALDI Ion Trap Mass Spectrometry. (ASMS 2004)        
Glyco-protein Purification / desalting 2010 Hiroto Hirayama, Junichi Seino, Toshihiko Kitajima, Yoshifumi Jigami and Tadashi Suzuki Free Oligosaccharides to Monitor Glycoprotein Endoplasmic Reticulum-associated Degradation in Saccharomyces cerevisiae The Journal of Biological Chemistry,   285,  12390-12404. Click "For desalting of PA-oligosaccharide-containing fractions, we used
C18+ carbon NuTip (Hypercrb, Glygen, Columbia, MD) as described previously"
NuTip C18+HyperCarb
Oligo-saccharide Purification / desalting 2009 Raphaël Carapito, Christine Carapito, Jean-Marc Jeltsch and Vincent Phalip, Bioresource Technology, Volume 100, Issue 2, January 2009, Pages 845-850, Efficient hydrolysis of hemicellulose by a Fusarium graminearum xylanase blend produced at high levels in Escherichia coli Click "...the polysaccharides were desalted using a NuTip type P20 carbon solid-phase extraction pipette tip (Glygen, USA)." NuTip P20
Oligo-saccharide Purification / desalting 2005 Lei Zhu, et.al Production of human monoclonal antibody in eggs of chimeric chickens, Nature Biotechnology 23, 1159 - 1169 (2005) Click "The released carbohydrates were desalted on a micro-carbon column from Glygen essentially following the vendor's protocol" TopTip Hypercarb
Oligo-saccharide Purification / desalting 2009 Raphael Carapito, Christine Carapito, Jean-Marc Jeltsch, Vincent Phalip, Efficient hydrolysis of hemicellulose by a Fusarium graminearum xylanase blend produced at high levels in Escherichia coli, Bioresource Technology, Volume 100, Issue 2, January 2009, Pages 845-850. Click "...the polysaccharides were desalted using a NuTip type P20 carbon solid-phase extraction pipette tip (Glygen, USA)." NuTip P-20
Protein Digestion 2007 Václav eovský , Jan Pohl , Zhihua Yang , Naseer Alam, Athula, B. Attygalle, Identification of three novel peptides isolated from the venom of the neotropical social wasp Polistes major major, Journal of Peptide Science Volume 13, Issue 7 (2007), Pages 445 – 450 Click "PMM2 dissolved in ammonium bicarbonate buffer (20 µl, 50 mM, pH 7.8) was aspirated into a NuTip Trypsin (Bovine) tip (Glygen Corp., MD, USA) and kept for 5 min." NuTip Trypsin
Protein Digestion 2005 Ashok K. Shukla; Mukta M Shukla; Alexis A. Oetting; Nelli I. Taranenko; Vladimir M. Doroshenko; Application of Non-Covalently Bound Trypsin on Chromatographic Material for Digestion of Proteins, ASMS 2005 TP-09, poster 144        
Protein Enrichment 2008 Kenneth M. Wannemacher, Alexandra Terskiy, Shengjie Bian, Prem N. Yadav, Hong Li, Richard D. Howells, Purification and mass spectrometric analysis of the [kappa] opioid receptor, Brain Research, Volume 1230, 16 September 2008, Pages 13-26. Click   NuTip C18
Protein Enrichment 2008 Protein Hydrolysate Compositions Having Improved Sensory Characteristics and Physical Properties , United States Patent Application 20080305212. Click      
Protein Enrichment 2008 S Hansen et al. Antibodies against insulin-like growth factor I receptor and uses thereof United States Patent Application 20080226635. Click      
Protein Enrichment 2008 Joshua F. Alfaro, Laura A. Gillies, He G. Sun, Shujia Dai, Tianzhu Zang, Joshua J. Klaene, Byung Ju Kim, Jonathan D. Lowenson, Steven G. Clarke, Barry L. Karge and Zhaohui Sunny Zhou, Chemo-Enzymatic Detection of Protein Isoaspartate Using Protein Isoaspartate Methyltransferase and Hydrazine Trapping, Anal. Chem., 2008, 80 (10), pp 3882–3889. Click "Centrifuge adapters purchased from Glygen (Columbia, MD) were used to suspend columns above 1.5 mL centrifuge tubes during centrifugation. " Centrifuge adaptors  
Protein Enrichment 2007 Emma Lundberg, Mårten Sundberg, Torbjörn Gräslund, Mathias Uhlén and Helene Andersson Svahn. A novel method for reproducible fluorescent labeling of small amounts of antibodies on solid phase. doi:10.1016/j.jim.2007.01.023, Journal of Immunological Methods Volume 322, Issues 1-2, 30 April 2007, Pages 40-49 Click "Micropipette tips (TopTip 10–200 μl) filled with protein A affinity medium were obtained from Glygen Corp." TopTip Protein A
Protein Enrichment 2007 Sandra E. Wiley, Mark L. Paddock, Edward C. Abresch, Larry Gross, Peter van der Geer, Rachel Nechushtai, Anne N. Murphy, Patricia A. Jennings and Jack E. Dixon. The Outer Mitochondrial Membrane Protein mitoNEET Contains a Novel Redox-active 2Fe-2S Cluster, J. Biol. Chem., Vol. 282, Issue 33, 23745-23749, August 17, 2007 Click "Untagged mitoNEET 33–108 (0.25 μm) was desalted by applying 20 μl of sample to a solid phase extraction pipette tip (TopTip, Glygen Corp.) containing 30 μl of Poros20 reverse phase C18-polymer resin" TopTip POROS RP2
Protein Enrichment 2003 Andrew J. Alpert and Ashok K. Shukla. Precipitation of Large Proteins from Serum with Organic Solvemts. ASMS 2003        
Protein Purification 2010 Wim Fremout, Maarten Dhaenens, Steven Saverwyns, Jana Sanyova, Peter Vandenabeele, Dieter Deforce, Luc Moens, Tryptic peptide analysis of protein binders in works of art by liquid chromatography-tandem mass spectrometry, Analytica Chimica Acta, Volume 658, Issue 2, 25 January 2010, Pages 156-162. Click   TopTip HILIC
Protein Purification 2009 On-Chip Solid-Phase Extraction Preconcentration/Focusing Substrates Coupled to Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization Ion Trap Mass Spectrometry for High Sensitivity Biomolecule Analysis. Arti Navare, Marcela Nouzova, Fernando G. Noriega, Salvador Hernández-Martínez, Christoph Menzel, and Facundo M. Fernández.  Rapid Commun Mass Spectrom. 2009 February; 23(4): 477–486.  Click   NuTip C18
Protein Purification 2009 David R.H. Evans, Jonathan K. Romero, Matthew Westoby, Chapter 9 Concentration of Proteins and Removal of Solutes, In: Richard R. Burgess and Murray P. Deutscher, Editor(s), Methods in Enzymology, Academic Press, 2009, Volume 463, Pages 97-120. Click "Examples of micropipette-based products are … NuTips (Glygen, Columbia, MD) in which the interior walls of the tip are coated with the binding." NuTip  
Protein Purification / desalting 2009 Kristin R. Wildsmith, Bomie Han and Randall J. Bateman, Analytical Biochemistry
Volume 395, Issue 1, 1 December 2009, Pages 116-118, Method for the simultaneous quantitation of apolipoprotein E isoforms using tandem mass spectrometry.
Click "Before analysis, samples were desalted using Carbon Nu-tips following the manufacturer's instructions (Glygen, Columbia, MD)." NuTip Carbon
Protein Purification / desalting 2009 Mahmoud Toorchi, Kiyoshi Yukawa, Mohammad-Zaman Nouri, Setsuko Komatsu, Proteomics approach for identifying osmotic-stress-related proteins in soybean roots, Peptides, Volume 30, Issue 12, December 2009, Pages 2108-2117. Click "..desalted with NuTip C-18 pipette tips (Glygen, Columbia, MD)" NuTip C18
Protein Purification / desalting 2009 Setsuko Komatsu, Takuya Wada, Yann Abal, Mohammad-Zaman Nouri, Yohei Nanjo, Norikazu Nakayama, Satoshi Shimamura, Ryo Yamamoto, Takuji Nakamura and Kiyoshi Furukawa , Analysis of Plasma Membrane Proteome in Soybean and Application to Flooding Stress Response, J. Proteome Res., 2009, 8 (10), pp 4487–4499. Click "...desalted with NuTip C-18 pipet tips (Glygen, Columbia, MD)" NuTip C18
Protein Purification / desalting 2009 KR Wildsmith, B Han, RJ Bateman. Method for the simultaneous quantitation of apolipoprotein E isoforms using tandem mass spectrometry. Analytical Biochemistry, Volume 395, Issue 1, 1 December 2009, Pages 116-118, Click "…Samples were desaltedusing Carbon Nu-tips following the manufacturer's instructions (Glygen, Columbia, MD)" NuTip HyperCarb
Small molecule Enrichment 2009 Siegel Marshall M: "GPC Spin Column HPLC–ESI-MS Methods for Screening Drugs Noncovalently Bound to Proteins," Ligand-Macromolecular Interactions in Drug Discovery: Methods and Protocols, 2009. Click   TopTip  
Small molecule Enrichment 2007 MM Siegel - Mass spectrometry in medicinal chemistry, 2007 - books.google.com Drug Screening Using Gel Permeation Chromatography Spin Columns Coupled with ESI        
    2009 Sebastian Leidel, Patrick G. A. Pedrioli, Tamara Bucher, Renée Brost, Michael Costanzo, Alexander Schmidt, Ruedi Aebersold, Charles Boone, Kay Hofmann& Matthias Peter, Ubiquitin-related modifier Urm1 acts as a sulphur carrier in thiolation of eukaryotic transfer RNA, Nature 458, 228-232 (12 March 2009) Click   NuTip TiO2
    2009 Serum biomarker profiling by solid-phase extraction with particle-embedded micro tips and matrix-assisted laser desorption/ionization mass spectrometry. Arti Navare, Manshui Zhou, John McDonald, Fernando G. Noriega, M. Cameron Sullards, and Facundo M. Fernandez. Rapid Commun Mass Spectrom. Author manuscript; available in PMC 2009 April 6. Click      
    2009 Stable Oxygen Isotope Labeling of Pre-existing Phosphoryl Groups on Phosphomolecules for Modification-Specific Mass Spectrometry United States Patent Application 20090142849.        
    2008 Brian Roberts, Jan Pohl, Jennifer L. Gooch, A fluorimetric method for determination of calcineurin activity, Cell Calcium, Volume 43, Issue 5, May 2008, Pages 515-519. Click "Titanium-oxide (TiO 4 )-coated plates were obtained from Glygen (Colombus, MA)" Coated plates TiO2
    2007 Koji Ikegam, Robb L. Heier, Midori Taruishi, , Hiroshi Takagi, Masahiro Mukai, Shuichi Shimma, Shu Taira, Ken Hatanaka , Nobuhiro Morone, Ikuko Yao,  Patrick K. Campbell, Shigeki Yuasa, Carsten Janke, Grant R. MacGregor , and Mitsutoshi Setou, Loss of -tubulin polyglutamylation in ROSA22 mice is associated with abnormal targeting of KIF1A and modulated synaptic function, PNAS | February 27, 2007 | vol. 104 | no. 9 | 3213-3218 Click "An anion exchange tip (Nutip; Glygen, Columbia, MD) was used to desalt the sample." NuTip Anion Exchange
    2007 Gerrit J. Schut, Stephanie L. Bridger, and Michael W. W. Adams, Insights into the Metabolism of Elemental Sulfur by the Hyperthermophilic Archaeon Pyrococcus furiosus: Characterization of a Coenzyme A- Dependent NAD(P)H Sulfur Oxidoreductase, Journal of Bacteriology, June 2007, p. 4431-4441, Vol. 189, No. 12 Click "Peptides were purified using NuTip
C 18 tips (Glygen Corp., Columbia, MD) and spotted (1 µl, containing -cyano-4-hydroxycinnamic acid) directly on a matrix-assisted laser desorption ionization plate."
NuTip C18
    2007  Angie R. Purvis, Julia Gross, Luke T. Dang, Ren-Huai Huang, Milan Kapadia, R. Reid Townsend, and J. Evan Sadler: Two Cys residues essential for von Willebrand factor multimer assembly in the Golgi, PNAS October 2, 2007 vol. 104 no. 40 15647-15652. Click "Reactions were desalted by adsorption on C18 micropipette tips (Glygen Corp., Columbia, MD) and elution with 60% acetonitrile/0.1% formic acid." NuTip C18
    2007 Ashok K. Shukla; Mukta Shukla: Reversed Gel Filtration for the Sample Preparation. (POSTER ASMS 2007)        
    2006 Mukta M. Shukla, Ashok K. Shukla, Nelli I. Taranenko, Vladimir M. Doroshenko, Sample Concentrator for AP-MALDI Plate. ASMS 2006.        
    2005 Christopher J. Toher , Adam W. Perala , Ashok K. Shukla , Gary A. Valaskovic Sample Purification for Static Nanospray MS Using Wall-Coated Pipette Trap'nTips™ , | (ASMS 2005)        
    2005 MM Shukla, AK Shukla, G Barka, N Manohar, NI Taranenko Use of Chromatographic Hollow Fiber for Purification and Separation of Peptides for AP-MALDI, ABRF 2005; P37-S. Click      
    2004 Tassilo Muskat, Ph.D. Thesis 2004. Matrixabhangiges Ablationsverhalten von Neutralen und Oberflahencharakteristika bei der Matrix Assisted Laser Desorption and Ionisation. Click   NuTip Carbon
    2004 Mukta M. Shukla , Ashok K. Shukla, Vladimir M. Doroshenko, Nelli I. Taranenko Nanoliter Solid Phase Extraction (SPE) Using Chromatographic Hollow Fibers for Sample Preparation for Mass Spectrometry (ASMS 2004)        
    2003 Ashok K. Shukla, Mukta M. Shukla, Eric D. Stover, and Andreas F. Huhmer. Application of Mixed-Mode Solid-PhaseExtraction (MMSPE) for Proteomics Sample Preparation. AMERICAN BIOTECHNOLOGY LABORATORY / OCTOBER 2003 Click   NuTip HyperCarb + C18
    2003 Mukta M. Shukla, Ashok K. Shukla, Eric D. Stover, and Andreas F. Huhmer. Chromatographic Hollow Fiber. ASMS 2003