Publications

Scientists from all over the world trust Glygen products for their most important experiments. Below is a list of the newest publications that have successfully used Glygen.

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MOST RECENT PUBLICATIONS

Year	Molecule	Application	Publication	Link	Quote	Glygen product	Media
2010	Glyco-peptide	Enrichment	Susan Grass, Cheryl F. Lichti, R. Reid Townsend, Julia Gross, and Joseph W. St. Geme, III The Haemophilus influenzae HMW1C Protein Is a Glycosyltransferase That Transfers Hexose Residues to Asparagine Sites in the HMW1 Adhesin PLoS Pathog. 2010 May; 6(5): e1000919.	Click	"Peptides were acidified with 5.5 цl formic acid (Sigma) and extracted 6 times with 10–200 цl NuTip porous graphite carbon wedge tips (Glygen) according to the manufacturer's directions and were then eluted into 1.5 ml autosampler vials with 60% acetonitrile"	NuTip	HyperCarb
2010	Glyco-protein	Purification / desalting	Hiroto Hirayama, Junichi Seino, Toshihiko Kitajima, Yoshifumi Jigami and Tadashi Suzuki Free Oligosaccharides to Monitor Glycoprotein Endoplasmic Reticulum-associated Degradation in Saccharomyces cerevisiae The Journal of Biological Chemistry, 285, 12390-12404.	Click	"For desalting of PA-oligosaccharide-containing fractions, we used C18+ carbon NuTip (Hypercrb, Glygen, Columbia, MD) as described previously"	NuTip	C18+HyperCarb
2010	Glyco-protein	Enrichment	Mizuho INAGAKI, Shuuichi NAKAYA, Daisuke NOHARA, Tomio YABE, Yoshihiro KANAMARU and Tohru SUZUKI, “The Multiplicity of N-Glycan Structures of Bovine Milk 18 kDa Lactophorin (Milk GlyCAM-1)”, Biosci. Biotechnol. Biochem., Vol. 74, 447-450 (2010) .	Click	"The PA-derivatized glycans were then dried with a centrifugal evaporator and dissolved in 10 цl of 0.1% v/v trifluoroacetic acid, followed by purified via Nutip Carbon (Glygen, Columbia, MD) according to the manufacturer's protocol."	NuTip	HyperCarb
2010	Glyco-protein	Enrichment	John W. Froehlich, Eric D. Dodds, Mariana Barboza, Erica L. McJimpsey, Richard R. Seipert, Jimi Francis, Hyun Joo An, Samara Freeman, J. Bruce German, Carlito B. Lebrilla Glycoprotein Expression in Human Milk during Lactation Journal of Agricultural and Food Chemistry 2010 58 (10), 6440-6448	Click	"Porous graphitized carbon cartridges were obtained from Glygen. "	NuTip	HyperCarb
2010	Glyco-protein	Enrichment	Ming Lei, et al, 'Sequential Enrichment of Sulfated Glycans by Strong Anion-Exchange Chromatography Prior to Mass Spectrometric Measurements,' J Am Soc Mass Spectrom, 2010, 21, 348-357	Click	"Sequential enrichment of sulfated glycans is attained using spin-columns packed with strong anion exchanger"	TopTip	SAX
2010	Peptide	Purification / desalting	Fan-Jiang Konga, Atsushi Oyanagia and Setsuko Komatsu Cell wall proteome of wheat roots under flooding stress using gel-based and LC MS/MS-based proteomics approaches Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics Volume 1804, Issue 1, January 2010, Pages 124-136	Click	"The peptide solution was desalted with NuTip C-18 pipet tips (Glygen, Columbia, MD, USA)."	NuTip	C18
2010	Peptide	Purification / desalting	Mohammad-Zaman Nouri, Setsuko Komatsu Comparative analysis of soybean plasma membrane proteins under osmotic stress using gel-based and LC MS/MS-based proteomics approaches PROTEOMICS Volume 10, Issue 10, pages 1930–1945, No. 10 May 2010	Click	"The resulting peptides were concentrated and desalted using a NuTip C-18 pipet tips (Glygen, Columbia, MD, USA) and analyzed by nanoLC MS/MS for protein identification."	NuTip	C18
2010	Peptide	Purification / desalting	Jian-Hua XIONG, Bin-Ying FU, Hua-Xue XU, and Yang-Sheng LI, Proteomic analysis of PEG-simulated drought stress responsive proteins of rice leaves using a pyramiding rice line at the seedling stage Botanical Studies (2010) 51: 137-145.	Click	"The peptide solution thus obtained was dried and reconcentrated with 30 ml of 0.1% TFA in 5% ACN/water and then desalted with NuTip C-18 pipette tips (Glygen, USA)."	NuTip	C18
2010	Peptide	Purification / desalting	Afroz, Amber; Rashid Khan, Muhammad; Komatsu, Setsuko Determination of Proteins Induced in Response to Jasmonic Acid and Salicylic Acid in Resistant and Susceptible Cultivars of Tomato Protein and Peptide Letters, Volume 17, Number 7, July 2010 , pp. 836-846(11)	Click	"The resulting peptide solution was dried and reconstituted with 30 μL of 0.1% trifluoroacetic acid in 5% acetonitrile and then desalted with NuTip C18 pipette tips (Glygen, Columbia, MD, USA)."	NuTip	C18
2010	Peptide	Purification / desalting	Afroz, Amber; Hashiguchi, Akiko; Khan, Muhammad R.; Komatsu, Setsuko Analyses of the Proteomes of the Leaf, Hypocotyl, and Root of Young Soybean Seedlings Protein and Peptide Letters, Volume 17, Number 3, March 2010 , pp. 319-331(13)	Click	"The resulting peptide solution was dried and reconstituted with 30 μL of 0.1% trifluoroacetic acid in 5% acetonitrile and then desalted with NuTip C18 pipette tips (Glygen, Columbia, MD)"	NuTip	C18
2010	Peptide	Purification / desalting	Yoshiaki Furukawa, Kumi Kaneko, Koji Yamanaka and Nobuyuki Nukina Mutation-dependent polymorphism of Cu,Zn-superoxide dismutase aggregates in the familial form of amyotrophic lateral sclerosis Journal of Biological Chemistry April 19, 2010	Click	"...desalted using NuTip C-18 (Glygen Co.)"	NuTip	C18
2010	Peptide	Purification	N Ahsan, Y Nanjo, H Sawada, Y Kohno, S Komatsu. Ozone stress-induced proteomic changes in leaf total soluble and chloroplast proteins of soybean reveal that carbon allocation is involved in adaptation in the early developmental stage. PROTEOMICS, Volume 10, Issue 14, pages 2605–2619, No. 14 July 2010	Click	"The generated peptides were purified using a NuTip C-18 (Glygen, Columbia, MD, USA)."	NuTip	C18
2010	Peptide	Purification	Junsong Sun, Robert C. Hopkins, Francis E. Jenney, Jr, Patrick M. McTernan, and Michael W. W. Adams Heterologous Expression and Maturation of an NADP-Dependent [NiFe]-Hydrogenase: A Key Enzyme in Biofuel Production PLoS One. 2010; 5(5): e10526.	Click	"The peptides were purified with C-18 reversed-phase NuTip® cartridges according to the manufacturer's instructions (Glygen Corp., Columbia, MD). "	NuTip	C18
2010	Peptide	Purification	Hamid Sobhanian, Roya Razavizadeh, Yohei Nanjo, Ali Akbar Ehsanpour, Ferdous Rastgar Jazii, Nasrin Motamed, Setsuko Komatsu Proteome analysis of soybean leaves, hypocotyls and roots under salt stress Proteome Science 2010, 8:19.	Click	"The generated peptides were purified using NuTip C-18 columns (Glygen Corp., Columbia, MD, USA)."	NuTip	C18
2010	Peptide	Purification	A Cvetkovic, AL Menon, MP Thorgersen, JW Scott, et al Microbial metalloproteomes are largely uncharacterized Nature 466, 779-782 (5 August 2010)	Click	"The peptides were purified with C-18 reversed-phase NuTip cartridges according to the manufacturer's instructions (Glygen). "	NuTip	C18
2010	Peptide	Purification	Thalia Nittis, Lionel Guittat, Richard D. LeDuc, Ben Dao, Julien P. Duxin, Henry Rohrs, R. Reid Townsend and Sheila A. Stewart. Revealing Novel Telomere Proteins Using in Vivo Cross-linking, Tandem Affinity Purification, and Label-free Quantitative LC-FTICR-MS Mol Cell Proteomics 2010 9: 1144-1156.	Click	"The final eluates were forced through Filter-in-a-tips (Glygen Corp., Columbia, MD) to remove any remaining beads."	TopTip	Filter
2010	Peptide	Enrichment	Ventura CL, Malachowa N, Hammer CH, Nardone GA, Robinson MA, et al. 2010 Identification of a Novel Staphylococcus aureus Two-Component Leukotoxin Using Cell Surface Proteomics. PLoS ONE 5(7): e11634. doi:10.1371/journal.pone.0011634	Click	"The dried sample was dissolved in 50 µl of 50 mM formic acid/75% isopropanol and applied to polyhydroxyethyl A 10-µl solid phase extraction tip (Glygen Corporation, Columbia, MD). "	NuTip	Poly-Hydroxy-ethyl A
2010	Peptide	Enrichment	Wen-Ping Chen, Xiao-Yuan Yang, Adrian D. Hegeman, William M. Gray, and Jerry D. Cohen Microscale analysis of amino acids using gas chromatography–mass spectrometry after methyl chloroformate derivatization Journal of Chromatography B Volume 878, Issue 24, 15 August 2010, Pages 2199-2208	Click	"Alternatively, commercial SCX tips (TT2-TWSCX.96, Glygen Corp, Columbia, MD, USA), based on their modified Top-tip format, proved successful and are more convenient for routine use."	TopTip	SCX
2010	Peptide	Enrichment	Scott J Walmsley, Corey Broeckling, Ann Hess, Jessica Prenni, and Norman P. Curthoys Proteomic Analysis of Brush Border Membrane Vesicles Isolated from Purified Proximal Convoluted Tubules Am J Physiol Renal Physiol (March 10, 2010). doi:10.1152/ajprenal.00711.2009	Click	"For SCX fractionation, the peptides were bound to a 156 Polysulfoethyl-A microspin TopTip (Glygen) and eluted stepwise with 20 μl volumes of 157 increasing ammonium acetate (20, 40, 60, 80, 120, 160 and 200 mM) in 20% acetonitrile, pH 158"	TopTip	SSA
2010	Phospho-peptide	Enrichment	Vishram P. Kedar, Martyn K. Darby, Jason G. Williams, and Perry J. Blackshear Phosphorylation of Human Tristetraprolin in Response to Its Interaction with the Cbl Interacting Protein CIN85 PLoS One. 2010; 5(3): e9588.	Click	"For phosphopeptide enrichment, TiO2 tips (Glygen) were employed using essentially the manufacturer's recommended protocol."	NuTip	TiO2
2010	Phospho-peptide	Enrichment	Mikkat S, Lorenz P, Scharf C, Yu X, Glocker MO, Ibrahim SM. MS characterization of qualitative protein polymorphisms in the spinal cords of inbred mouse strains. Proteomics. 2010 Mar;10(5):1050-62.	Click	"Porous titanium dioxide immobilized to the inner surface of pipette tips (NuTipTM, Glygen, Columbia, MD, USA) was used for the selective enrichment of phosphorylated peptides. "	NuTip	TiO2
2010	Phospho-peptide	Enrichment	John D. Leszyk Evaluation of the New MALDI Matrix 4-Chloro-α-Cyanocinnamic Acid J Biomol Tech. 2010 July; 21(2): 81–91.	Click	"Phosphopeptides were enriched using a modification of the published method.7 The dried peptide digest was dissolved in 10 μl binding solvent (75:19:6 acetonitrile:water:TFA). A 1- to 10-μl TiO2 NuTip was washed two times with 10 μl aliquots of binding solvent. Phosphopeptides were bound by repetitive pipetting (20 times) of the 10-μl sample volume. The NuTip was then washed five times with binding solvent and then five times with 0.1% TFA. Phosphopeptides were then eluted in a separate tube with a 10-μl vol 6% NH4OH after repetitive pipetting (10 times). A 5-μl aliquot of 5% TFA was then added, and the phosphopeptides were concentrated further and purified prior to MALDI analysis using a micro C18 ZipTip."	NuTip	TiO2
2010	Phospho-peptide	Enrichment	Matthew B. Gatesa, Kenneth B. Tomera and Leesa J. Deterding. Comparison of Metal and Metal Oxide Media for Phosphopeptide Enrichment Prior to Mass Spectrometric Analyses. JASMS June 2010.	Click	"Both MALDI and ESI-MS analyses of the TiO2 NuTip eluents resulted in the reproducible observation of a greater number of unique sites of phosphorylation with the least amount of nonspecific binding compared with the other MOAC resins. ... Enrichment procedures before MS analyses dramatically improve detection and sequencing of phosphopeptides..."	NuTip	TiO2
2010	Phospho-peptide	Enrichment	Uma K. Aryal, Andrew R. S. Ross. Enrichment and analysis of phosphopeptides under different experimental conditions using titanium dioxide affinity chromatography and mass spectrometry. Rapid Communications in Mass Spectrometry Volume 24, Issue 2, pages 219–231, 30 January 2010.	Click	"To address these uncertainties, we have evaluated the performance of TiO2-MOAC under different loading and elution conditions, using a single make and model of commercially available TiO2 column (NuTipTM NT1TIO, Glygen Corp., USA) to minimize experimental variability."	NuTip	TiO2
2010	Phospho-peptide	Enrichment	Jamie D. Dunn, Gavin E. Reid, Merlin L. Bruening Techniques for phosphopeptide enrichment prior to analysis by mass spectrometry Mass Spectrometry Reviews, Volume 29, Issue 1, pages 29–54, January 2010	Click	"...Microtips that contained zirconium dioxide (Glygen, Columbia, MD) were utilized for the enrichment of phosphorylated peptides, and the specificity and recovery achieved was compared to that obtained with Glygen microtips containing titanium dioxide (with the same binding, rinsing and elution solutions)... Overall, the TiO2 microtips were more selective for enrichment of multiply phosphorylated peptides, whereas the ZrO2 tips enriched primarily monophosphorylated peptides. "	NuTip and TopTip	TiO2
2010	Phospho-peptide	Enrichment	Parvathi Rudrabhatla, Philip Grant, Howard Jaffe, Michael J. Strong, and Harish C. Pant Quantitative phosphoproteomic analysis of neuronal intermediate filament proteins (NF-M/H) in Alzheimer’s disease by iTRAQ FASEB J..2010; 0: fj.10-157859v1	Click	"...phopeptide enrichment by TiO2 chromatography utilizing a 1- to 200-l TiO2 Top Tip (Glygen Corp., Columbia, MD, USA) essentially according to the method of Wu et al. "	TopTip	TiO2
2010	Phospho-peptide	Enrichment	Özlü, Nurhan and Monigatti, Flavio and Renard, Bernhard Y. and Field, Christine M. and Steen, Hanno and Mitchison, Timothy J. and Steen, Judith J. Binding Partner Switching on Microtubules and Aurora-B in the Mitosis to Cytokinesis Transition Mol Cell Proteomics 2010 9: 336-350. First Published on September 28, 2009, doi:10.1074/mcp.M900308-MCP200	Click	"TiO 2 was used to enrich for phosphopeptides. Briefly, lyophilized samples were dissolved in binding buffer (5% TFA, 40% acetonitrile). After loading samples onto the TiO 2 columns (TopTip, Glygen), the column was washed with the binding buffer."	TopTip	TiO2
2010	Phospho-peptide	Enrichment	Weitao Jia, Justin F. Shaffer, Samantha P. Harris, Julie A. Leary Identification of Novel Protein Kinase A Phosphorylation Sites in the M-domain of Human and Murine Cardiac Myosin Binding Protein-C Using Mass Spectrometry Analysis Journal of Proteome Research 2010 9 (4), 1843-1853	Click	"The phosphopeptides were also enriched using TopTips filled with TiO 2 (particle size 20−30 μm, Glygen Corp., Columbia, MD) as described in the manufacturer's instruction with minor modifications."	TopTip	TiO2
2010	Phospho-peptide	Enrichment	Michael Mazanek, Elisabeth Roitinger, Otto Hudecz, James R.A. Hutchins, Bjorn Hegemann, Goran Mitulovic, Thomas Taus, Christoph Stingl, Jan-Michael Peters, Karl Mechtler, A new acid mix enhances phosphopeptide enrichment on titanium- and zirconium dioxide for mapping of phosphorylation sites on protein complexes, Journal of Chromatography B, Volume 878, Issues 5-6, 15 February 2010, Pages 515-524.	Click			TiO2/ZrO2
2010	Protein	Purification	Wim Fremout, Maarten Dhaenens, Steven Saverwyns, Jana Sanyova, Peter Vandenabeele, Dieter Deforce, Luc Moens, Tryptic peptide analysis of protein binders in works of art by liquid chromatography-tandem mass spectrometry, Analytica Chimica Acta, Volume 658, Issue 2, 25 January 2010, Pages 156-162.	Click		TopTip	HILIC

PUBLICATIONS BY APPLICATION

Molecule	Application	Year	Publication	Link	Quote	Glygen product	Media
Peptide	Enrichment	2010	Ventura CL, Malachowa N, Hammer CH, Nardone GA, Robinson MA, et al. 2010 Identification of a Novel Staphylococcus aureus Two-Component Leukotoxin Using Cell Surface Proteomics. PLoS ONE 5(7): e11634. doi:10.1371/journal.pone.0011634	Click	"The dried sample was dissolved in 50 µl of 50 mM formic acid/75% isopropanol and applied to polyhydroxyethyl A 10-µl solid phase extraction tip (Glygen Corporation, Columbia, MD). "	NuTip	Poly-Hydroxy-ethyl A
Peptide	Enrichment	2010	Wen-Ping Chen, Xiao-Yuan Yang, Adrian D. Hegeman, William M. Gray, and Jerry D. Cohen Microscale analysis of amino acids using gas chromatography–mass spectrometry after methyl chloroformate derivatization Journal of Chromatography B Volume 878, Issue 24, 15 August 2010, Pages 2199-2208	Click	"Alternatively, commercial SCX tips (TT2-TWSCX.96, Glygen Corp, Columbia, MD, USA), based on their modified Top-tip format, proved successful and are more convenient for routine use."	TopTip	SCX
Peptide	Enrichment	2010	Scott J Walmsley, Corey Broeckling, Ann Hess, Jessica Prenni, and Norman P. Curthoys Proteomic Analysis of Brush Border Membrane Vesicles Isolated from Purified Proximal Convoluted Tubules Am J Physiol Renal Physiol (March 10, 2010). doi:10.1152/ajprenal.00711.2009	Click	"For SCX fractionation, the peptides were bound to a 156 Polysulfoethyl-A microspin TopTip (Glygen) and eluted stepwise with 20 μl volumes of 157 increasing ammonium acetate (20, 40, 60, 80, 120, 160 and 200 mM) in 20% acetonitrile, pH 158"	TopTip	SSA
Peptide	Enrichment	2009	Fang Shen, Nan Li, Padmaja Gade, Dhananjaya V. Kalvakolanu, Timothy Weibley, Brad Doble, James R. Woodgett, Troy D. Wood, and Sarah L. Gaffen, IL-17 Receptor Signaling Inhibits C/EBPβ by Sequential Phosphorylation of the Regulatory 2 Domain, Sci. Signal., 24 February 2009 Vol. 2, Issue 59, p. ra8	Click	"…Vacuum-dried peptides were dissolved in 80%ACN/0.1%TFA and loaded on a Top tip-TiO 2 micropipette tip (Glygen Corp)"	TopTip	TiO2
Peptide	Enrichment	2009	Proteomic analyses of native brain KV4.2 channel complexes. Céline Marionneau, Richard D. LeDuc, Henry W. Rohrs, Andrew J. Link, R. Reid Townsend, and Jeanne M. Nerbonne. Proteomic analyses of native brain KV4.2 channel complexes. 2009 Jul–Aug; 3(4): 284–294.Published online 2009 July 16.	Click	"Peptides were acidified with formic acid, extracted with NuTip porous graphite carbon wedge tips (Glygen)…"	NuTip	HyperCarb
Peptide	Enrichment	2008	R . Craig-Schapiro, A . Davis, R . LeDuc, A . Sha , D . Holtzman, R . Townsend , A . Fagan: Development of a solid phase extraction protocol for extracting peptides from cerebrospinal fluid in conjunction with tandem mass spectrometry to identify novel biomarkers for Alzheimer's disease Alzheimer's and Dementia , Volume 4, Issue 4, Supplement 1, July 2008, Pages T536-T537.			NuTip	Carbon
Peptide	Enrichment	2007	Jurre J. Kamphorst,Rob van der Heijden, Jeroen DeGroot, Floris P. J. G. Lafeber, Theo H. Reijmers,Benno van El,Ubbo R. Tjaden, Jan van der Greef, and Thomas Hankemeier, Profiling of Endogenous Peptides in Human Synovial Fluid by NanoLC?MS: Method Validation and Peptide Identification, J. Proteome Res., 2007, 6 (11), pp 4388–4396.	Click	"SPE was performed with POROS RP-2 C 18 TopTips (Glygen, Columbia, MD)"	NuTip	POROS RP2
Peptide	Enrichment	2007	Gordon B. Mitchell, Muthafar H. Al-Haddawi, Mary Ellen Clark, Jennifer D. Beveridge, and Jeff L. Caswell. Effect of Corticosteroids and Neuropeptides on the Expression of Defensins in Bovine Tracheal Epithelial Cells. Infection and Immunity, March 2007, p. 1325-1334, Vol. 75, No. 3.	Click	"Samples were concentrated by C18 TopTip (Glygen Corp., Columbia, MD)"	TopTip	C18
Peptide	Enrichment	2004	Wolfgang H. Fischer, Rebecca Ross, Minkyu Park, Chien Li, Cynthia Donaldson and Joan Vaughan. Identification of Processing Sites in Neuropeptides Employing Microaffinity Columns and MALDI-MS (ASMS 2004). used TopTip with Carboxyl group for the EDC coupling applications			TopTip	Affinity
Peptide	Enrichment	2004	Andrew J. Alpert and Ashok K. Shukla (ABRF 2004 Poster), Displacement Chromatography Effects Can Cause Highly Selective Sampling of Peptides During Solid Phase Extraction Cleanup.	Click
Peptide	Purificadtion	2008	Jing-hua Xi, Fang Bai, Julia Gross , R. Reid Townsend, A. Sue Menko, and Usha P. Andley, A KNOCK-IN MOUSE MODEL DEMONSTRATES THAT THE R49C MUTATION IN A-CRYSTALLIN ENHANCES PROTEIN INSOLUBILITY AND CELL DEATH, J. Biol. Chem., Vol. 283, Issue 9, 5801-5814, February 29, 2008.	Click	"...digest was diluted with an equal volume of water and the peptides were extracted with a NuTip porous graphite carbon tip (Glygen Part No. NP2CAR.96)"	NuTip	Carbon
Peptide	Purification	2010	N Ahsan, Y Nanjo, H Sawada, Y Kohno, S Komatsu. Ozone stress-induced proteomic changes in leaf total soluble and chloroplast proteins of soybean reveal that carbon allocation is involved in adaptation in the early developmental stage. PROTEOMICS, Volume 10, Issue 14, pages 2605–2619, No. 14 July 2010	Click	"The generated peptides were purified using a NuTip C-18 (Glygen, Columbia, MD, USA)."	NuTip	C18
Peptide	Purification	2010	Junsong Sun, Robert C. Hopkins, Francis E. Jenney, Jr, Patrick M. McTernan, and Michael W. W. Adams Heterologous Expression and Maturation of an NADP-Dependent [NiFe]-Hydrogenase: A Key Enzyme in Biofuel Production PLoS One. 2010; 5(5): e10526.	Click	"The peptides were purified with C-18 reversed-phase NuTip® cartridges according to the manufacturer's instructions (Glygen Corp., Columbia, MD). "	NuTip	C18
Peptide	Purification	2010	Hamid Sobhanian, Roya Razavizadeh, Yohei Nanjo, Ali Akbar Ehsanpour, Ferdous Rastgar Jazii, Nasrin Motamed, Setsuko Komatsu Proteome analysis of soybean leaves, hypocotyls and roots under salt stress Proteome Science 2010, 8:19.	Click	"The generated peptides were purified using NuTip C-18 columns (Glygen Corp., Columbia, MD, USA)."	NuTip	C18
Peptide	Purification	2010	A Cvetkovic, AL Menon, MP Thorgersen, JW Scott, et al Microbial metalloproteomes are largely uncharacterized Nature 466, 779-782 (5 August 2010)	Click	"The peptides were purified with C-18 reversed-phase NuTip cartridges according to the manufacturer's instructions (Glygen). "	NuTip	C18
Peptide	Purification	2010	Thalia Nittis, Lionel Guittat, Richard D. LeDuc, Ben Dao, Julien P. Duxin, Henry Rohrs, R. Reid Townsend and Sheila A. Stewart. Revealing Novel Telomere Proteins Using in Vivo Cross-linking, Tandem Affinity Purification, and Label-free Quantitative LC-FTICR-MS Mol Cell Proteomics 2010 9: 1144-1156.	Click	"The final eluates were forced through Filter-in-a-tips (Glygen Corp., Columbia, MD) to remove any remaining beads."	TopTip	Filter
Peptide	Purification	2009	Roya Razavizadeh, Ali Akbar Ehsanpour, Nagib Ahsan, Setsuko Komatsu, Proteome analysis of tobacco leaves under salt stress, Peptides, Volume 30, Issue 9, September 2009, Pages 1651-1659.	Click	"Purification of the generated peptides was achieved using NuTip C-18 (Glygen, Columbia, MD, USA)."	NuTip	C18
Peptide	Purification	2009	S. Komatsu, E. Yamada and K. Furukawa Cold stress changes the concanavalin A-positive glycosylation pattern of proteins expressed in the basal parts of rice leaf sheaths, Amino Acids (2009), 0939-4451 (Print) 1438-2199 (Online).	Click	"Purification of the generated peptides was achieved using Nutip C-18 (Glygen, Columbia, MD, USA). "	NuTip	C18
Peptide	Purification	2009	Setsuko Komatsu, Tetsuya Sugimoto, Tomoki Hoshino, Yohei Nanjo and Kiyoshi Furukawa Identification of flooding stress responsible cascades in root and hypocotyl of soybean using proteome analysis Amino Acids Volume 38, Number 3, 729-738, DOI: 10.1007/s00726-009-0277-0	Click	"The degenerated peptides were purified using NuTip C-18 (Glygen, Columbia, MD, USA)."	NuTip	C18
Peptide	Purification	2009	S. Komatsu, E. Yamada and K. Furukawa, Cold stress changes the concanavalin A-positive glycosylation pattern of proteins expressed in the basal parts of rice leaf sheaths, Amino Acids, Volume 36, Number 1 / January, 2009	Click	"Purification of the generated peptides was achieved using Nutip C-18 (Glygen, Columbia, MD, USA"	NuTip	C18
Peptide	Purification / desalting	2009	T. Mahmood, A. Jan and S. Komatsu Biologia Plantarum, Volume 53, Number 2 / June, 2009, 285-293, Proteomic analysis of bacterial blight defence signalling pathway using transgenic rice overexpressing thaumatin-like protein.	Click	"The peptide solution, thus obtained was dried and reconcentrated with 0.03 cm3 of 0.1 TFA in 5 % acetonitrile/water and then desalted with NuTip C-18 pipette tips (Glygen, Columbia, MD, USA)."	NuTip	C18
Peptide	Purification / desalting	2008	Hideaki Shimizu, Asako Tosaki, Kumi Kaneko, Tamao Hisano, Takashi Sakurai, and Nobuyuki Nukina Crystal, Structure of an Active Form of BACE1, an Enzyme Responsible for Amyloid ? Protein Production, Molecular and Cellular Biology, June 2008, p. 3663-3671, Vol. 28, No. 11	Click	"desalted with a hydrophilic Nutip cartridge"	NuTip	HILIC
Peptide	Purification / desalting	2008	Extensive contacts between ADAMTS13 exosites and von Willebrand factor domain A2 contribute to substrate specificity. Weiqiang Gao, Patricia J. Anderson, and J. Evan Sadler. Blood. 2008 September 1; 112(5): 1713–1719.	Click	"Reactions were stopped with EDTA, and then desalted by adsorption on C18 micropipette tips (Glygen, Columbia, MD) and elution with 60% acetonitrile/0.1% formic acid."	NuTip	C18
Peptide	Purification / desalting	2008	Julia Gross, Susan Grass, Alan E. Davis, Petra Gilmore-Erdmann, R. Reid Townsend and Joseph W. St. Geme III, The Haemophilus influenzae HMW1 Adhesin Is a Glycoprotein with an Unusual N-Linked Carbohydrate Modification, First Published on July 11, 2008, doi: 10.1074/jbc.M801819200 September 19, 2008 The Journal of Biological Chemistry, 283, 26010-26015.	Click	"All digestions were acidified to 5% formic acid, and desalted peptides were prepared using a NuTip carbon tip (Glygen) according to the manufacturer's instructions."	NuTip	HyperCarb
Peptide	Purification / desalting	2008	Fang Shi, Ryo Yamamoto, Satoshi Shimamura, Susumu Hiraga, Norikazu Nakayama, Takuji Nakamura, Kiyoshi Yukawa, Mayumi Hachinohe, Hiroshi Matsumoto, Setsuko Komatsu, Cytosolic ascorbate peroxidase 2 (cAPX 2) is involved in the soybean response to flooding, Phytochemistry, Volume 69, Issue 6, April 2008, Pages 1295-1303.	Click		NuTip	C18
Peptide	Purification / desalting	2007	Xin Zang and Setsuko Komatsu, A proteomics approach for identifying osmotic-stress-related proteins in rice, Phytochemistry, Volume 68, Issue 4, February 2007, Pages 426-437	Click		NuTip	C18
Peptide	Purification / desalting	2006	Weiqiang Gao, Patricia J. Anderson, Elaine M. Majerus, Elodee A. Tuley, and J. Evan Sadler, Exosite interactions contribute to tension-induced cleavage of von Willebrand factor by the antithrombotic ADAMTS13 metalloprotease, PNAS \| December 12, 2006 \| vol. 103 \| no. 50 \| 19099-19104.	Click	"Reactions were stopped with EDTA, and then desalted by adsorption on C18 micropipette tips (Glygen, Columbia, MD) and elution with 60% acetonitrile/0.1% formic acid."	NuTip	C18
Peptide	Purification / desalting	2006	Tariq Mahmood , Asad Jan, Makoto Kakishima, Setsuko Komatsu, Proteomic analysis of bacterial-blight defense-responsive proteins in rice leaf blades Proteomics Volume 6, Issue 22 (2006), Pages 6053 - 6065	Click	"The peptide solution thus obtained was dried and reconcen- trated with 30 mL of 0.1% TFA in 5% ACN/water and then desalted with NuTip C-18 pipette tips (Glygen, Columbia, MD, USA)."	NuTip	C18
Peptide	Purification / desalting	2006	Tadashi Suzuki, Izumi Hara, Miyako Nakano, Masaki Shigeta, Takatoshi Nakagawa, Akihiro Kondo, Yoko Funakoshi and Naoyuki Taniguchi, Man2C1, an a-mannosidase is involved in the trimming of free oligosaccharides in the cytosol. Biochem. J. (2006)	Click	"The mixture was desalted using C18+Carbon NuTip (Hypercarb; Glygen Co., Columbia MD)."	NuTip	Carbon+C18
Peptide	Purification / desalting	2006	Hao Wang, Jennifer E. Grant, Christopher M. Doede, Sakthivel Sadayappan, Jeffrey Robbins, Jeffery W. Walker, PKC-[beta]II sensitizes cardiac myofilaments to Ca2+ by phosphorylating troponin I on threonine-144, Journal of Molecular and Cellular Cardiology, Volume 41, Issue 5, November 2006, Pages 823-833.	Click	"The two samples were combined, desalted on TopTip microcolumns (Glygen, Inc.) and analyzed by MALDI-TOF mass spectrometry"	TopTip	C18
Peptide	Purification / desalting	2006	Michael Wacker, Mario F. Feldman, Nico Callewaert, Michael Kowarik, Bradley R. Clarke\|\|, Nicola L. Pohl, Marcela Hernandez, Enrique D. Vines, Miguel A. Valvano, Chris Whitfield, and Markus Aebi,Substrate specificity of bacterial oligosaccharyltransferase suggests a common transfer mechanism for the bacterial and eukaryotic systems. PNAS \| May 2, 2006 \| vol. 103 \| no. 18 \| 7088-7093	Click	"Tryptic peptides extracted from in-gel-digested SDS/PAGE bands were desalted over a NuTip 10 Carbon cleanup tip (Glygen, Columbia, MD)."	TopTip	Hypercarb
Peptide	Purification / desalting	2005	Oleg Chertov, John T Simpson, Arya Biragyn, Thomas P Conrads, Timothy D Veenstra and Robert J Fisher, Enrichment of low-molecular-weight proteins from biofluids for biomarker discoveryExpert Review of Proteomics, January 2005, Vol. 2, No. 1, Pages 139-145 .	Click	"TopTip™ pipet tip (Glygen Corp.) is more efficient in separating precipitate and soluble material than centrifugation."	NuTip	C18
Peptide	Purification / desalting	2003	A.J. Alpert,A.K. Shukla, ABRF 2003 (2/2003; Denver): Poster# P111-W, Precipitation of Large, High-Abundance Proteins from Serum with Organic Solvents.	Click	"TopTip™disposable SPE pipet tip packed with C-18-coated silica (bed volume 25 μl) [Glygen Corp. item# TT2C18] was conditioned with two washes of 50 μl ACN."	TopTip	C18
Peptide	Purification / desalting	1999	E.T. Chin and D.I.Papac. The Use of Porous Graphite Carbon Column for Desalting Hydropilic peptides prior to Matrix Assisted Desorption/ Ionization Time-of-Flight Mass Spectrometry Analytical Biochemistry 1999, 273, 179-185.			TopTip	Hypercarb
Peptide	Purification / desalting	2010	Fan-Jiang Konga, Atsushi Oyanagia and Setsuko Komatsu Cell wall proteome of wheat roots under flooding stress using gel-based and LC MS/MS-based proteomics approaches Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics Volume 1804, Issue 1, January 2010, Pages 124-136	Click	"The peptide solution was desalted with NuTip C-18 pipet tips (Glygen, Columbia, MD, USA)."	NuTip	C18
Peptide	Purification / desalting	2010	Mohammad-Zaman Nouri, Setsuko Komatsu Comparative analysis of soybean plasma membrane proteins under osmotic stress using gel-based and LC MS/MS-based proteomics approaches PROTEOMICS Volume 10, Issue 10, pages 1930–1945, No. 10 May 2010	Click	"The resulting peptides were concentrated and desalted using a NuTip C-18 pipet tips (Glygen, Columbia, MD, USA) and analyzed by nanoLC MS/MS for protein identification."	NuTip	C18
Peptide	Purification / desalting	2010	Jian-Hua XIONG, Bin-Ying FU, Hua-Xue XU, and Yang-Sheng LI, Proteomic analysis of PEG-simulated drought stress responsive proteins of rice leaves using a pyramiding rice line at the seedling stage Botanical Studies (2010) 51: 137-145.	Click	"The peptide solution thus obtained was dried and reconcentrated with 30 ml of 0.1% TFA in 5% ACN/water and then desalted with NuTip C-18 pipette tips (Glygen, USA)."	NuTip	C18
Peptide	Purification / desalting	2010	Afroz, Amber; Rashid Khan, Muhammad; Komatsu, Setsuko Determination of Proteins Induced in Response to Jasmonic Acid and Salicylic Acid in Resistant and Susceptible Cultivars of Tomato Protein and Peptide Letters, Volume 17, Number 7, July 2010 , pp. 836-846(11)	Click	"The resulting peptide solution was dried and reconstituted with 30 μL of 0.1% trifluoroacetic acid in 5% acetonitrile and then desalted with NuTip C18 pipette tips (Glygen, Columbia, MD, USA)."	NuTip	C18
Peptide	Purification / desalting	2010	Afroz, Amber; Hashiguchi, Akiko; Khan, Muhammad R.; Komatsu, Setsuko Analyses of the Proteomes of the Leaf, Hypocotyl, and Root of Young Soybean Seedlings Protein and Peptide Letters, Volume 17, Number 3, March 2010 , pp. 319-331(13)	Click	"The resulting peptide solution was dried and reconstituted with 30 μL of 0.1% trifluoroacetic acid in 5% acetonitrile and then desalted with NuTip C18 pipette tips (Glygen, Columbia, MD)"	NuTip	C18
Peptide	Purification / desalting	2010	Yoshiaki Furukawa, Kumi Kaneko, Koji Yamanaka and Nobuyuki Nukina Mutation-dependent polymorphism of Cu,Zn-superoxide dismutase aggregates in the familial form of amyotrophic lateral sclerosis Journal of Biological Chemistry April 19, 2010	Click	"...desalted using NuTip C-18 (Glygen Co.)"	NuTip	C18
Peptide	Purification / desalting	2009	Akiko Hashiguchi, Katsumi Sakata and Setsuko Komatsu, Proteome Analysis of Early-Stage Soybean Seedlings under Flooding Stress, J. Proteome Res., 2009, 8 (4), pp 2058–206	Click	"The peptide solution obtained was dried and reconcentrated with 30 μL of 0.1% trifluioroacetic acid in 50% acetonitrile and desalted with NuTip C-18 pipet tips (Glygen, Columbia, MD)."	NuTip	C18
Peptide	Purification / desalting	2009	Bekim Bajrami, Yu Shi, Pascal Lapierre, Xudong Yao, Shifting Unoccupied Spectral Space in Mass Spectrum of Peptide Fragment Ions, Journal of the American Society for Mass Spectrometry, Volume 20, Issue 11, November 2009, Pages 2124-2134.	Click	"The resulting peptides were desalted using HLB material (Waters), which was packed in-house in TopTips (10–200 μL; Glygen, Columbia, MD"	TopTip	Empty
Peptide	Purification / desalting	2009	Nisar Ahmad Khan, Ryoji Takahashi, Jun Abe, Setsuko Komatsu, Identification of cleistogamy-associated proteins in flower buds of near-isogenic lines of soybean by differential proteomic analysis, Peptides, Volume 30, Issue 12, December 2009, Pages 2095-2102 (Corrected Proof - Note to users, doi:10.1016/j.peptides.2009.08.012.)	Click	"The peptide solution obtained was dried and reconcentrated with 30 μL of 0.1% trifluoroacetic acid in 50% acetonitrile and desalted with NuTip C-18 pipette tips (Glygen, Columbia, MD, USA)."	NuTip	C18
Peptide	Purification / desalting	2009	Setsuko Komatsu, Ryo Yamamoto, Yohei Nanjo, Yoji Mikami, Harunobu Yunokawa and Katsumi Sakata , A Comprehensive Analysis of the Soybean Genes and Proteins Expressed under Flooding Stress using Transcriptome and Proteome Techniques, J. Proteome Res., 2009, 8 (10), pp 4766–4778.	Click	"...peptide solution obtained was dried and reconcentrated with 30 μL of 0.1% trifluioroacetic acid in 50% acetonitrile and desalted with NuTip C-18 pipet tips (Glygen, Columbia, MD)"	NuTip	C18
Peptide	Purification / desalting	2009	Mahmood, Tariq; Kakishima, Makoto; Komatsu, Setsuko, Protein and Peptide Letters, Volume 16, Number 9, September 2009 , pp. 1041-1052(12), Proteome Analysis of Probenazole-Effect in Rice-Bacterial Blight Interactions.	Click	"The peptide solution, thus obtained was dried, reconcentrated and desalted with NuTip C-18 pipett tips (Glygen, Columbia, MD, USA)."	NuTip	C18
Peptide	Purification / desalting	2008	Toshihiro Ishizawa, Yusuke Nozaki, Takuya Ueda, Nono Takeuchi, The human mitochondrial translation release factor HMRF1L is methylated in the GGQ motif by the methyltransferase HMPrmC, Biochemical and Biophysical Research Communications, Volume 373, Issue 1, 15 August 2008, Pages 99-103.	Click	"The peptides were further purified using a Nutip C-18 column (Glygen Corp.) before MALDI-TOF MS analysis"	NuTip	C18
Peptide	Purification / desalting	2008	YanWen Feng , Setsuko Komatsu, Toshiko Furukawa, Tomokazu Koshiba and Yoshihisa Kohno Proteome analysis of proteins responsive to ambient and elevated ozone in rice seedlings , Agriculture, Ecosystems & Environment, Volume 125, Issues 1-4, May 2008, Pages 255-265.	Click		NuTip	C18
Peptide		2008	Benfang Ruan, et. al. Binding of rapamycin analogs to calcium channels and FKBP52 contributes to their neuroprotective activities, PNAS January 8, 2008 vol. 105 no. 1 33-38	Click	"TopTip P-4 column was from Glygen (Columbia, MD)"	TopTip	P-4 (Gel filtration)
Phospho-peptide	Enrichment	2010	Vishram P. Kedar, Martyn K. Darby, Jason G. Williams, and Perry J. Blackshear Phosphorylation of Human Tristetraprolin in Response to Its Interaction with the Cbl Interacting Protein CIN85 PLoS One. 2010; 5(3): e9588.	Click	"For phosphopeptide enrichment, TiO2 tips (Glygen) were employed using essentially the manufacturer's recommended protocol."	NuTip	TiO2
Phospho-peptide	Enrichment	2010	Mikkat S, Lorenz P, Scharf C, Yu X, Glocker MO, Ibrahim SM. MS characterization of qualitative protein polymorphisms in the spinal cords of inbred mouse strains. Proteomics. 2010 Mar;10(5):1050-62.	Click	"Porous titanium dioxide immobilized to the inner surface of pipette tips (NuTipTM, Glygen, Columbia, MD, USA) was used for the selective enrichment of phosphorylated peptides. "	NuTip	TiO2
Phospho-peptide	Enrichment	2010	John D. Leszyk Evaluation of the New MALDI Matrix 4-Chloro-α-Cyanocinnamic Acid J Biomol Tech. 2010 July; 21(2): 81–91.	Click	"Phosphopeptides were enriched using a modification of the published method.7 The dried peptide digest was dissolved in 10 μl binding solvent (75:19:6 acetonitrile:water:TFA). A 1- to 10-μl TiO2 NuTip was washed two times with 10 μl aliquots of binding solvent. Phosphopeptides were bound by repetitive pipetting (20 times) of the 10-μl sample volume. The NuTip was then washed five times with binding solvent and then five times with 0.1% TFA. Phosphopeptides were then eluted in a separate tube with a 10-μl vol 6% NH4OH after repetitive pipetting (10 times). A 5-μl aliquot of 5% TFA was then added, and the phosphopeptides were concentrated further and purified prior to MALDI analysis using a micro C18 ZipTip."	NuTip	TiO2
Phospho-peptide	Enrichment	2010	Matthew B. Gatesa, Kenneth B. Tomera and Leesa J. Deterding. Comparison of Metal and Metal Oxide Media for Phosphopeptide Enrichment Prior to Mass Spectrometric Analyses. JASMS June 2010.	Click	"Both MALDI and ESI-MS analyses of the TiO2 NuTip eluents resulted in the reproducible observation of a greater number of unique sites of phosphorylation with the least amount of nonspecific binding compared with the other MOAC resins. ... Enrichment procedures before MS analyses dramatically improve detection and sequencing of phosphopeptides..."	NuTip	TiO2
Phospho-peptide	Enrichment	2010	Uma K. Aryal, Andrew R. S. Ross. Enrichment and analysis of phosphopeptides under different experimental conditions using titanium dioxide affinity chromatography and mass spectrometry. Rapid Communications in Mass Spectrometry Volume 24, Issue 2, pages 219–231, 30 January 2010.	Click	"To address these uncertainties, we have evaluated the performance of TiO2-MOAC under different loading and elution conditions, using a single make and model of commercially available TiO2 column (NuTipTM NT1TIO, Glygen Corp., USA) to minimize experimental variability."	NuTip	TiO2
Phospho-peptide	Enrichment	2010	Jamie D. Dunn, Gavin E. Reid, Merlin L. Bruening Techniques for phosphopeptide enrichment prior to analysis by mass spectrometry Mass Spectrometry Reviews, Volume 29, Issue 1, pages 29–54, January 2010	Click	"...Microtips that contained zirconium dioxide (Glygen, Columbia, MD) were utilized for the enrichment of phosphorylated peptides, and the specificity and recovery achieved was compared to that obtained with Glygen microtips containing titanium dioxide (with the same binding, rinsing and elution solutions)... Overall, the TiO2 microtips were more selective for enrichment of multiply phosphorylated peptides, whereas the ZrO2 tips enriched primarily monophosphorylated peptides. "	NuTip and TopTip	TiO2
Phospho-peptide	Enrichment	2010	Parvathi Rudrabhatla, Philip Grant, Howard Jaffe, Michael J. Strong, and Harish C. Pant Quantitative phosphoproteomic analysis of neuronal intermediate filament proteins (NF-M/H) in Alzheimer’s disease by iTRAQ FASEB J..2010; 0: fj.10-157859v1	Click	"...phopeptide enrichment by TiO2 chromatography utilizing a 1- to 200-l TiO2 Top Tip (Glygen Corp., Columbia, MD, USA) essentially according to the method of Wu et al. "	TopTip	TiO2
Phospho-peptide	Enrichment	2010	Özlü, Nurhan and Monigatti, Flavio and Renard, Bernhard Y. and Field, Christine M. and Steen, Hanno and Mitchison, Timothy J. and Steen, Judith J. Binding Partner Switching on Microtubules and Aurora-B in the Mitosis to Cytokinesis Transition Mol Cell Proteomics 2010 9: 336-350. First Published on September 28, 2009, doi:10.1074/mcp.M900308-MCP200	Click	"TiO 2 was used to enrich for phosphopeptides. Briefly, lyophilized samples were dissolved in binding buffer (5% TFA, 40% acetonitrile). After loading samples onto the TiO 2 columns (TopTip, Glygen), the column was washed with the binding buffer."	TopTip	TiO2
Phospho-peptide	Enrichment	2010	Weitao Jia, Justin F. Shaffer, Samantha P. Harris, Julie A. Leary Identification of Novel Protein Kinase A Phosphorylation Sites in the M-domain of Human and Murine Cardiac Myosin Binding Protein-C Using Mass Spectrometry Analysis Journal of Proteome Research 2010 9 (4), 1843-1853	Click	"The phosphopeptides were also enriched using TopTips filled with TiO 2 (particle size 20−30 μm, Glygen Corp., Columbia, MD) as described in the manufacturer's instruction with minor modifications."	TopTip	TiO2
Phospho-peptide	Enrichment	2010	Michael Mazanek, Elisabeth Roitinger, Otto Hudecz, James R.A. Hutchins, Bjorn Hegemann, Goran Mitulovic, Thomas Taus, Christoph Stingl, Jan-Michael Peters, Karl Mechtler, A new acid mix enhances phosphopeptide enrichment on titanium- and zirconium dioxide for mapping of phosphorylation sites on protein complexes, Journal of Chromatography B, Volume 878, Issues 5-6, 15 February 2010, Pages 515-524.	Click			TiO2/ZrO2
Phospho-peptide	Enrichment	2009	R. Luke Wiseman, King-Tung Chin, Cole M. Haynes1 Ariel Stanhill, Chong-FengXu, Assen Roguev, Nevan J. Krogan, Thomas A. Neubert and David Ron: hioredoxin related protein 32 is an arsenite-regulated thiol reductase of the proteasome 19S particle, JBC Papers in Press. Published on April 6, 2009	Click	"For the phospho-SILAC experiments, the extracted peptides were further purified on a TiO 2 tip (1-10-μl NuTip; Glygen)."	NuTip	Carbon
Phospho-peptide	Enrichment	2009	MVS Sivaram, TL Wadzinski, SD Redick, T Manna, SJ: Dynein light intermediate chain 1 is required for progress through the spindle assembly chckpoint. The EMBO Journal, 2009 – natue.com.	Click	"Phosphorylated tryptic peptides were enriched for using NuTip TiO2 micropipette tips (Glygen Corporation) using a modified protocol"	NuTip	TIO2
Phospho-peptide	Enrichment	2009	Mingquan Guo: A phosphoproteomic strategy compatible with systems biology analysis, doi:10.1016/j.ab.2009.04.008 Anal. Biochem.	Click	"TiO 2 particles (50 μm) were purchased from Glygen (Columbia, MD, USA)."	Media	TiO2
Phospho-peptide	Enrichment	2009	R. Luke Wiseman, King-Tung Chin, Coe M. Haynes, Ariel Stanhill, Chong-Feng Xu, Assen Roguev, Nevan J. Krogan, Thomas A. Neubert, and David Ron: Thioredoxin-related Protein 32 Is an Arsenite-regulated Thiol Reductase of the Proteasome 19 S Particle J. Biol. Chem., Vol. 284, Issue 22, 15233-15245, May 29, 2009	Click	"For the phospho-SILAC experiments, the extracted peptides were further purified on a TiO 2 tip (1-10-μl NuTip; Glygen)."	NuTip	TIO2
Phospho-peptide	Enrichment	2009	Anne M. Distler, Janos Kerner, Kwangwon Lee, Charles L. Hoppel, Chapter 6 Modifications of Mitochondrial Outer Membrane Proteins, In: William S. Allison and Anne N. Murphy, Editor(s), Methods in Enzymology, Academic Press, 2009, Volume 457, Mitochondrial Function, Part B: Mitochondrial Protein Kinases, Protein Phosphatases and Mitochondrial Diseases, Pages 97-115.	Click	"The porous titanium and zirconium oxide microtips are purchased from Glygen (Columbia, MD)."	NuTip	TiO2/ZrO2
Phospho-peptide	Enrichment	2009	Mingquan Guo, A phosphoproteomic strategy compatible with systems biology analysis, Analytical Biochemistry, In Press, Corrected Proof, Available online 11 April 2009.	Click	"TiO 2 particles (50 μm) were purchased from Glygen (Columbia, MD, USA)."	Media	TiO2
Phospho-peptide	Enrichment	2009	Ryan Chong, Rachel Swiss, Gabriel Briones, Kathryn L. Stone, Erol E. Gulcicek, Herve Agaisse, Regulatory Mimicry in Listeria monocytogenes Actin-Based Motility, Cell Host & Microbe, Volume 6, Issue 3, 17 September 2009, Pages 268-278.	Click		NuTip	TiO2
Phospho-peptide	Enrichment	2009	Jesse Rinehart, Yelena D. Maksimova, Jessica E. Tanis, Kathryn L. Stone, Caleb A. Hodson, Junhui Zhang, Mary Risinger, Weijun Pan, Dianqing Wu, Christopher M. Colangelo, Biff Forbush, Clinton H. Joiner, Erol E. Gulcicek, Patrick G. Gallagher, Richard P. Lifton, Sites of Regulated Phosphorylation that Control K-Cl Cotransporter Activity, Cell, Volume 138, Issue 3, 7 August 2009, Pages 525-536.	Click	"Peptides were extracted with 0.5% trifluoroacetic acid, dried, resuspended, and applied to a TiO2 TopTip micro-spin column (Glygen Corp.)."	TopTip	TiO2
Phospho-peptide	Enrichment	2009	Zenglan Wang, Gaofeng Dong, Sasha Singh, Hanno Steen, Jiaxu Li, A simple and effective method for detecting phosphopeptides for phosphoproteomic analysis, Journal of Proteomics, Volume 72, Issue 5, Special Section: "From Genome to Proteome: Integration and proteome completion", 21 July 2009, Pages 831-835.	Click	"The peptide solution was then added to 2.6 mg of equilibrated TiO 2 beads (Glygen) in a microspin column…"	Media	TiO2
Phospho-peptide	Enrichment	2009	Chitra Rajagopal, Kathryn L. Stone, Victor P. Francone, Richard E. Mains and Betty A. Eipper , Secretory Granule to the Nucleus, ROLE OF A MULTIPLY PHOSPHORYLATED INTRINSICALLY UNSTRUCTURED DOMAIN, First Published on July 27, 2009, doi: 10.1074/jbc.M109.035782 September 18, 2009 The Journal of Biological Chemistry, 284, 25723-25734.	Click	" TiO 2 Top Tips (Glygen Corp.) were washed with 40 μl of 100% acetonitrile, followed by 0.2 m sodium phosphate, pH 7.0, and then by 0.5% trifluoroacetic acid, 50% acetonitrile."	TopTip	TiO2
Phospho-peptide	Enrichment	2009	Emily S. Boja, Darci Phillips, Stephanie A. French, Robert A. Harris and Robert S. Balaban, Quantitative Mitochondrial Phosphoproteomics Using iTRAQ on an LTQ-Orbitrap with High Energy Collision Dissociation, J. Proteome Res., 2009, 8 (10), pp 4665–4675.	Click	"Titanium dioxide prepacked toptips were obtained from Glygen (Columbia, MD)."	TopTip	TiO2
Phospho-peptide	Enrichment	2009	Minsoo Oh, Hangun Kim, Ilhwan Yang, Ja-Hye Park, Wei-Tao Cong, Moon-Chang Baek, Sonja Bareiss, Hyunkyoung Ki, Qun Lu, Jinhyung No, Inho Kwon, Jung-Kap Choi and Kwonseop Kim, GSK-3 phosphorylates delta-catenin and negatively regulates its stability via ubiquitination/proteosome-mediated proteolysis, First Published on August 25, 2009, doi: 10.1074/jbc.M109.002659.	Click	"After trypsin digestion, phosphopeptides were enriched using phosphopeptide enrichment… TiO 2 (Glygen Corp.) according to the manufacturer's protocol."	TopTip	TiO2
Phospho-peptide	Enrichment	2008	Jamie D. Dunn, Elizabeth A. Igrisan, Amanda M. Palumbo, Gavin E. Reidand Merlin L. Bruening: Phosphopeptide Enrichment Using MALDI Plates Modified with High-Capacity Polymer Brushes, Anal. Chem., 2008, 80 (15), pp 5727–5735	Click	"Pipet tips containing 30 μg of TiO 2 or ZrO 2 embedded into the walls of the tip were purchased from Glygen. These systems have a binding capacity of 1−2 μg of peptide."	NuTip	TiO2/ZrO2
Phospho-peptide	Enrichment	2008	Chao Yuan, Quanhu Sheng,Haixu Tang, Yixue Li, Rong Zeng, and R. John Solaro, Quantitative comparison of sarcomeric phosphoproteomes of neonatal and adult rat hearts, Am J Physiol Heart Circ Physiol 295: H647-H656, 2008.	Click	"TiO 2 Nutips (Glygen, Columbia, MD) were washed with binding buffer (10% ACN-2% FA), then with 10 mg/ml dihydroxybenzoic (DHB; Sigma-Aldrich)…"	NuTip	TIO2
Phospho-peptide	Enrichment	2008	Marit Lenman, Carolin Sörensson, and Erik Andreasson Enrichment of Phosphoproteins and Phosphopeptide Derivatization Identify Universal Stress Proteins in Elicitor-Treated Arabidopsis. Molecular Plant-Microbe Interactions, October 2008, Volume 21, Number 10, Pages 1275-1284.	Click	"Prepacked TiO2 Nutip columns from Glygen…"	NuTip	TiO2
Phospho-peptide	Enrichment	2008	Matthias Rainer , Harald Sonderegger , Rania Bakry , Christian W. Huck , Sandra Morandell , Lukas A. Huber , Douglas T. Gjerde , Günther K. Bonn, Analysis of protein phosphorylation by monolithic extraction columns based on poly(divinylbenzene) containing embedded titanium dioxide and zirconium dioxide nano-powders. Proteomics Volume 8 Issue 21 (2008),Pages4593–4602	Click		TopTip	TiO2
Phospho-peptide	Enrichment	2008	Scott B. Ficarro, Jignesh R. Parikh, Nathaniel C. Blankand Jarrod A. Marto: Niobium(V) Oxide (Nb2O5): Application to Phosphoproteomics. Anal. Chem., 2008, 80 (12), pp 4606–4613	Click	"MALDI mass spectra of tryptic α-casein peptides enriched by TiO 2 and a mixed bed of TiO 2 and Nb 2 O 5 , each in TopTip format."	TopTip	TiO2
Phospho-peptide	Enrichment	2008	Matthew B. Gates, KennethB.Tomer and LeesaJ.Deterding: MALDI/MS Comparison of Fe-NTA Immobilized Metal Affinity Chromatography and Commercially-Available Metal Oxide Affinity Resins for Phosphopeptide Enrichment, NATO Science for Peace and Security Series A: Chemistry and Biology, Applications of Mass Spectrometry in Life Safety, p. 37-54 (2008).	Click	"Six different resins are compared: Glygen TiO2 NuTip, ZrO2 NuTip, mixed ZrO2 and TiO2 NuTip,Titansphere TiO2, PhosTrap magnetic titanium beads, and a Fe-NTA resin. "	NuTip and TopTip	TiO2/ZrO2
Phospho-peptide	Enrichment	2008	Eric S. Simon, Matthew Young, Antonia Chana, Zhao-Qin Bao and Philip C. Andrews, Improved enrichment strategies for phosphorylated peptides on titanium dioxide using methyl esterification and pH gradient elution, Analytical Biochemistry, Volume 377, Issue 2, 15 June 2008, Pages 234-242.	Click	"TiO 2 particles (50 μm) were purchased from Glygen (Columbia, MD)"	Media	TiO2
Phospho-peptide	Enrichment	2008	Mika Teranishi, Kentaro Nakamura, Hiroshi Morioka, Kazuo Yamamoto and Jun Hidema: The Native Cyclobutane Pyrimidine Dimer Photolyase of Rice Is Phosphorylated, Plant Physiology 146:1941-1951 (2008).	Click	"recover the phosphorylated peptides from tryptic peptide mixtures of the 56-kD CPD photolyase using a titanium dioxide microcolumn (Glygen)"	TopTip	TiO2
Phospho-peptide	Enrichment	2008	Adam J. Carroll, Joshua L. Heazlewood, Jun Ito, A. Harvey Millar, Analysis of the Arabidopsis cytosolic ribosome proteome provides detailed insights into its components and their post-translational modification, Journal of Biological Chemistry, 2008 ASBMB, ... 283, Issue 9, 5801-5814, February 29, 2008.	Click	"Phosphopeptide Enrichment Using Titanium Dioxide—. TiO 2 tips (NuTip) were supplied by Glygen Inc., and phosphopeptide enrichment procedures were essentially those outlined by Larsen et al. (24) with some modifications."	NuTip	TiO2
Phospho-peptide	Enrichment	2008	M Mazanek, G Mitulovi, F Herzog, C Stingl, JRA Titanium dioxide as a chemo-affinity solid phase in offline phosphopeptide chromatography prior to 11 enriched phosphopeptides by using self-packed TiO 2 tips, 2008.	Click	"enriched phosphopeptides by using self-packed TiO 2 tips, but we decided to use commercially available pre-packed "TopTips" from Glygen"	TopTip	TiO2
Phospho-peptide	Enrichment	2008	Liwen Wang; John C. Byrd; Michael A. Freitas and Raj Muthusamy. A Tandem Phosphoprotein / Phosphopeptide Enrichment Strategy for the Characterization of Signaling Proteins in Chronic Lymphocytic Leukemia.Presentation, Fifth Annual Ohio Mass Spectrometry Symposium March 24-25, 2008	Click	"Phosphopeptides were then enriched by use of zirconium dioxide coated NuTip (Glygen Corp, Columbia, MD)."	NuTip	ZrO2
Phospho-peptide	Enrichment	2008	Brian Roberts, Jan Pohl and Jennifer L. Gooch: A fluorimetric method for determination of calcineurin activity, Cell Calcium, Volume 43, Issue 5, May 2008, Pages 515-519.	Click	"Titanium-oxide (TiO 4 )-coated plates were obtained from Glygen (Colombus, MA)"	Lab in a Plate	TiO2
Phospho-peptide	Enrichment	2007	Henrik Molina, David M. Horn, Ning Tang, Suresh Mathivanan, and Akhilesh Pandey: Global proteomic profiling of phosphopeptides using electron transfer dissociation tandem mass spectrometry, PNAS February 13, 2007 vol. 104 no. 7 2199-2204.	Click	"The peptides obtained from the digestion step were dried down, and selected fractions were redissolved in 20 μl of 80% acetonitrile, 66 mg/ml 2,5-dihydrobenzoic acid, and 1% TFA and loaded onto a home-built TiO 2 (Glygen Corp, Columbia MD) microcolumn"	NuTip	TiO2
Phospho-peptide	Enrichment	2007	Eugen Damoc, Christopher S. Fraser, Min Zhou, Hortense Videler, Greg L. Mayeur, John W. B. Hershey, Jennifer A. Doudn, Carol V. Robinson, and Julie A. Leary : Structural Characterization of the Human Eukaryotic Initiation Factor 3 Protein Complex by Mass Spectrometry. Molecular & Cellular Proteomics 6:1135-1146, 2007.	Click	"Microtips filled with TiO2 were purchased from Glygen and used according to the manufacturer's instructions."	NuTip	TiO2
Phospho-peptide	Enrichment	2007	Xin-De Zheng, Raymond Teck Ho Lee, Yan-Ming Wang, Qi-Shan Lin and Yue Wang The EMBO Journal advance online publication 2 August 2007, Phosphorylation of Rga2, a Cdc42 GAP, by CDK/Hgc1 is crucial for Candida albicans hyphal growth. doi: 10.1038/sj.emboj.7601814, The EMBO Journal (2007), 1–10	Click	"The phosphopeptides were enriched using TiO2 TopTip (Glygen Inc., Columbia, NY). The bound peptides were eluted with 0.5% NH3OH."	TopTip	TiO2
Phospho-peptide	Enrichment	2007	D. A. Moraga, M. C. Chow, I. Isaac, and S. M. Stevens, P77-M Automated Analysis of Gel-Derived Phosphoproteins Using the Investigator Proteomic System, Jr. J Biomol Tech. 2007 February; 18(1): 26.	Click	"Tryptic digests from each protein spot were processed with the ProMS workstation using TiO2 microcolumns (Glygen Corp., Columbia, MD) for phosphopeptide enrichment prior to MALDI-TOF/TOF analysis (ABI 4700 Proteomics Analyzer)."	TopTip	TiO2
Phospho-peptide	Enrichment	2007	Liwen Wang, Hua Xu, Chen Ren, Shujun Liu, Guido Marcucci and Michael A. Freitas (Use of ZrO2 Tips)Enrichment and Characterization of Histone H1 Phosphorylation Isoforms in Chemoprevention of Acute Myeloid Leukemia. 4th Annual Ohio Mass Spectrometry Symposium (March 19-20 2007) 3rd Annual			NuTip	ZrO2
Phospho-peptide	Enrichment	2007	Eugen Damoc, Christopher S. Fraser, Min Zhou, Hortense Videler, Greg L. MayeurJohn W. B. Hershey, Jennifer A. Doudna, Carol V. Robinson and Julie A. Leary: Structural Characterization of the Human Eukaryotic Initiation Factor 3.	Click	"Microtips filled with TiO 2 were purchased from Glygen and used according to the manufacturer's instructions."	NuTip	TiO2
Phospho-peptide	Enrichment	2007	DJ Jang, M Guo, D Wang. Proteomic and biochemical studies of calcium-and phosphorylation-dependent calmodulin complexes in mammalian cells. J. Proteome Res., 2007, 6 (9), pp 3718–3728	Click	"Aliquots of the tryptic digests were enriched for phosphopeptides using TiO 2 columns (Glygen Inc.) as described 23 and analyzed by LC−MS/MS"		TiO2
Phospho-peptide	Enrichment	2006	Christopher J. Toher ; Adam W. Perala; Carla J. Marshall-Waggett; Gary A. Valaskovic; Ashok K. Shukla; A.A. Oetting; M.M. Shukla; N. Manohar. Online and Offline Nanoelectrospray Analysis of Phosphopeptides Purified by TiO2, ZrO2, and Carbon Wall-Coated Pipette Tips. ASMS 2006 Poster TP 215			NuTip	Carbon
Phospho-peptide	Enrichment	2006	H. K. Kweon and K. Hakansson. Selective zirconium dioxide-based enrichment of phosphorylated peptides for mass spectrometric analysis. Anal Chem. 2006 Mar 15;78(6):1743-9.	Click	"Microtips filled with ZrO2 and TiO2 (25 or 50 μg) ...from Glygen (Columbia, MD) [were] used without further modification."	NuTip	TiO2/ZrO2
Phospho-peptide	Enrichment	2006	Michael Mazanek, Goran Mituloviae, Franz Herzog, Christoph Stingl, James RA Hutchins, Jan Michael Peters and Karl Mechtler Titanium dioxide as a chemo-affinity solid phase in offline phosphopeptide chromatography prior to HPLC-MS/MS analysis	Click	"TiO2 -filled tips: TopTips, produced by Glygen"	TopTIp	TiO2
Phospho-peptide	Enrichment	2006	Christopher J. Toher, Adam W. Perala, Ashok K. Shukla, Gary A. Valaskovic Offline Nano-ESI Phosphopeptide Analysis with Carbon, TiO2, and ZrO2, wall-Coated Trap'nTips™, ABRF 2006			TopTip	TiO2
Phospho-peptide	Enrichment	2006	King JB, Gross J, Lovly CM, Rohrs H, Piwnica-Worms H, Townsend RR. Accurate mass-driven analysis for the characterization of protein phosphorylation. Study of the human Chk2 protein kinase.Anal Chem. 2006 Apr 1; 78(7):2171-81.	Click
Phospho-peptide	Enrichment	2006	Christopher J. Toher ; Adam W. Perala; Carla J. Marshall-Waggett; Gary A. Valaskovic; Ashok K. Shukla; A.A. Oetting; M.M. Shukla; N. Manohar. Online and Offline Nanoelectrospray Analysis of Phosphopeptides Purified by TiO2, ZrO2, and Carbon Wall-Coated Pipette Tips. ASMS 2006 Poster TP 215 ( for Hypercarb and TiO2)			NuTip and TopTip	POROS RP2
Phospho-peptide	Enrichment	2006	Maja Matis; Urs A. Meyer. Phosphoproteome analysis of primary human hepatocytes: effect of exposure to inducers of cytochromes P450. ASMS-2006 POSTER No. Th 658.			NuTip and TopTip	POROS RP2
Phospho-peptide	Enrichment	2006	Maja Matis, Ragna Sack and Urs A. Meyer. Role of protein phosphorylation/dephosphorylations in the regulation of cytochrome P450 genes ASMS 2006 POSTER No. 2666			NuTip and TopTip	POROS RP2
Phospho-peptide	Enrichment	2006	Qishan Lin, Jinghua Zhu. Application Titanium Dioxide IMAC for Enrichment Phosphopeptides Prior to Tandem Mass Spectrometry., U Albany Center for Functional Genomics	Click		NuTip and TopTip	POROS RP2
Phospho-peptide	Enrichment	2006	Julie B. King, Julia Gross, Christine M. Lovly, Henry Rohrs,Helen Piwnica-Worms, and R. Reid Townsend, Accurate Mass-Driven Analysis for the Characterization of Protein Phosphorylation. Study of the Human Chk2 Protein Kinase, Anal. Chem., 2006, 78 (7), pp 2171–2181.	Click
Phospho-peptide	Enrichment	2006	Christopher J. Toher, Adam W. Perala, Ashok K. Shukla, Gary A. Valaskovic: Offline Nano-ESI Phosphopeptide Analysis with TiO2, and ZrO2 Wall-Coated Trap'nTips™, ASMS 2006
Phospho-peptide	Enrichment	2004	Nelli I. Taranenko, Vladimir M. Doroshenko, Ashok K. Shukla, and Mukta M. Shukla. Applications of AP-MALDI Ion Trap Mass Spectrometry for the Analysis of Phosphopeptides, AMERICAN LABORATORY 36 MAY 2004	Click		NuTip	IMAC
Phospho-peptide	Enrichment	2004	V. M. Doroshenko, N. I. Taranenko, A. K. Shukla, M. M. Shukla Phospho- and Glyco-peptides Analysis Using Negative and Positive AP-MALDI Ion Trap Mass Spectrometry. ABRF2004
Phospho-peptide	Enrichment	2004	V. M. Doroshenko, N. I. Taranenko, A. K. Shukla, M. M. Shukla AP-MALDI Ion Trap Mass Spectrometry Analysis of Selective Phosphopeptides Using Different Immobilized Metal Affinity Chromatography Materials (ASMS 2004)
Phospho-peptide	Enrichment	2002	Loughrey Chen S, Huddleston MJ, Shou W, Deshaies RJ, Annan RS, Carr SA. Mass spectrometry-based methods for phosphorylation site mapping of hyperphosphorylated proteins applied to Net1, a regulator of exit from mitosis in yeast. Mol Cell Proteomics. 2002 Mar;1(3):186-96.			TopTip	Hypercarb
Phospho-peptide	Enrichment	1999	Jenny Albanese, Senior Field Applications Specialist ABI- Proteomics Phospho workshop at Mississippi State University: IMAC in combination with LC-Maldi enables femtomole detection of phosphopeptides. (1999) Profiling the Phosphoproteome of Endometrial Carcinoma.			NuTip and TopTip	POROS RP2
Phospho-peptide	Purification / desalting	2007	Andrew J. Alpert; Steven P. Gygi; Ashok K. Shukla, Desalting Phosphopeptides by Solid-Phase Extraction (POSTER ASMS 2007)
Phospho-peptide	Purification / desalting	2006	Adrian M. Taylor; Leroi DeSouza; K.W. Michael Siu Profiling the Phosphoproteome of Endometrial Carcinoma ASMS 2006. Poster No. TP462.	Click		TopTip	C18
Phospho-protein	Enrichment	2008	Scott B. Ficarro, Jignesh R. Parikh, Nathaniel C. Blank and Jarrod A. Marto, Niobium(V) Oxide (Nb2O5): Application to Phosphoproteomics,Anal. Chem., 2008, 80 (12), pp 4606–4613
Glyco-peptide	Enrichment	2010	Susan Grass, Cheryl F. Lichti, R. Reid Townsend, Julia Gross, and Joseph W. St. Geme, III The Haemophilus influenzae HMW1C Protein Is a Glycosyltransferase That Transfers Hexose Residues to Asparagine Sites in the HMW1 Adhesin PLoS Pathog. 2010 May; 6(5): e1000919.	Click	"Peptides were acidified with 5.5 цl formic acid (Sigma) and extracted 6 times with 10–200 цl NuTip porous graphite carbon wedge tips (Glygen) according to the manufacturer's directions and were then eluted into 1.5 ml autosampler vials with 60% acetonitrile"	NuTip	HyperCarb
Glyco-peptide	Enrichment	2008	Kazutosi Kubota, Yuji Sato, Yusuke Suzuki, Naoko Goto-Inoue, Tosifusa Toda, Minoru Suzuki, Shin-ichi Hisanaga, Akemi Suzuki and Tamao Endo, Analysis of Glycopeptides Using Lectin Affinity Chromatography with MALDI-TOF Mass Spectrometry, Anal. Chem., 2008, 80 (10), pp 3693–3698	Click	"Top Tips were purchased from Glygen Corp. (Columbia, MD)."	TopTip	Lectin
Glyco-peptide	Enrichment	2007	Carol L. Nilsson, Lectins: Analytical Tools from Nature, In: Carol L. Nilsson, Editor(s), Lectins, Elsevier Science B.V., Amsterdam, 2007, Pages 1-13.	Click		TopTip	Lectin
Glyco-peptide	Enrichment	2006	Vivianne I. Otto , Eugen Damoc, Leah N. Cueni, Thomas Schurpf, Renate Frei , Sarah Ali , Nico Callewaert , Adrian Moise, Julie A. Leary, Gerd Folkers, and Michael Przybylski: N-Glycan Structures and N-Glycosylation Sites of Mouse Soluble Intercellular Adhesion Molecule-1 Revealed by MALDI-TOF and FTICR Mass Spectrometry Glycobiology Advance Access published online on July 28, 2006 Glycobiology, doi:10.1093/glycob/cwl032	Click	"NuTip graphitized carbon 10-µL pipette tips were from Glygen (Columbia, MD)."	NuTip	Carbon
Glyco-peptide	Enrichment	2006	Joseph P. Connolly, Dr. , Diego Comerci , Timothy G. Alefantis , Alexander Walz , Marian Quan , Ryan Chafin, Paul Grewal Cesar V. Mujer, Rodolfo A. Ugalde, Vito G. DelVecchio. Proteomic analysis of Brucella abortus cell envelope and identification of immunogenic candidate proteins for vaccine development. PROTEOMICS Volume 6, Issue 13 (2006) , Pages 3767 – 3780	Click	"was resuspended in binding solution (15 mM ammonium acetate, pH 3.5, with 85% ACN) and loaded onto a hydrophilic interaction TopTip HILIC column"	TopTip	HILIC
Glyco-peptide	Enrichment	2006	T Adamson, K Hakansson, Infrared Multiphoton Dissociation and Electron Capture Dissociation of High Mannose-Type Glycopeptides". J. Proteome Res. 2006, 5, 493.	Click	"For the overnight trypsin digest, concanavalin A tips (Glygen Corporation, Columbia, MD) were used to selectively bind glycopeptides."	TopTip	Lectin
Glyco-peptide	Enrichment	2006	Wouter Laroy, Roland Contreras and Nico Callewaert. Glycome mapping on DNA, sequencing equipment. Nature Protocols 1, 397 - 405 (2006)	Click		NuTip	Carbon
Glyco-peptide	Enrichment	2004	Ashok K. Shukla, Mukta M. Shukla, Vladimir M. Doroshenko, Nelli I. Taranenko. Applications of Glycosidase in Glycoproteins Structure Determination by AP-MALDI MS/MS Analysis. ASMS 2004
Glyco-peptide	Purification	2009	Eric D. Dodds, Richard R. Seipert, Brian H. Clowers, J. Bruce Germanand Carlito B. Lebrilla, Analytical Performance of Immobilized Pronase for Glycopeptide Footprinting and Implications for Surpassing Reductionist Glycoproteomics, J. Proteome Res., 2009, 8 (2), pp 502–512	Click	"To compensate for the increased concentration of buffer salts, the digests were then purified by solid-phase extraction using 10 μL of Glygen NuTips loaded with graphitic carbon (Columbia, MD)."	NuTip	Carbon
Glyco-peptide	Purification	2004	N. I. Taranenko, A. K. Shukla, M. M. Shukla, V. M. Doroshenko. Effects of Lectins Affinity Chromatography on Glycoproteins Enrichment Using AP-MALDI Ion Trap Mass Spectrometry (2004)	Click	"After digestion, an aliquot of digested peptides and glycopeptides was applied to 10 µl immobilized lectin columns (TopTip)."	TopTip	Lectin
Glyco-peptide	Purification / desalting	2006	Takashiba M, Chiba Y, Jigami Y. Identification of phosphorylation sites in N-linked glycans by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Anal Chem. 2006 Jul 15;78(14):5208-13.	Click	"After HPLC separation, the phosphorylated glycans were purified using ion-exchange resin Muromac and applied to porous graphitic carbon (PGC) cartridges (Nutip Carbon and GL-Pak Carbograph) for desalting"	NuTip	Carbon
Glyco-peptide	Purification / desalting	2006	Kameyama A, Nakaya S, Ito H, Kikuchi N, Angata T, Nakamura M, Ishida HK, Narimatsu H, Strategy for simulation of CID spectra of N-linked oligosaccharides toward glycomics. J Proteome Res. 2006 Apr; 5(4):808-14.	Click	"The filtrates were desalted by using a NuTip-Hypercarbon (Glygen Corp., Columbia, MD) according to the manufacturer's manual."	NuTip	Hypercarb
Glyco-peptide	Purification / desalting	2006	Leah Cueni. A method for the qualitative and quantitative analysis of the glycosylation pattern of mouse soluble intercellular adhesion molecule-1 (sICAM-1), Institute of Pharmaceutical Sciences (Diploma Thesis), 2006	Click	"Samples were desalted prior to mass spectrometric analyses using NuTipTM graphitized car-bon 10μl pipette tips (Glygen Corp., Columbia, MD). Tips were conditioned by pipetting five times 10μl of 60% acetonitrile containing 0.05% trifluoroacetic acid (TFA) followed by three times 10μl of water."	NuTip	Carbon
Glyco-peptide	Purification / desalting	2009	Afucosylated antibodies against CCR5 and their use for the treatment of inflammatory conditions, United States Patent Application 20090226434.	Click	"Subsequently, the glycan structures released were isolated and desalted using NuTip-Carbon pipette tips (obtained from Glygen)."	NuTip	HyperCarb
Glyco-peptide	Purification / desalting	2008	Tadashi Suzuki, Ichiro Matsuo, Kiichiro Totani, Sho Funayama, Junichi Seino, Naoyuki Taniguchi, Yukishige Ito, Sumihiro Hase, Dual-gradient high-performance liquid chromatography for identification of cytosolic high-mannose-type free glycans, Analytical Biochemistry, Volume 381, Issue 2, 15 October 2008, Pages 224-232.	Click	"Fractions were desalted using C18 + Carbon NuTip (Hypercarb, Glygen, Columbia, MD)"	NuTip	Carbon
Glyco-peptide	Purification / desalting	2008	Julia Gross, Susan Grass, Alan E. Davis, Petra Gilmore-Erdmann, R. Reid Townsend, Joseph W. St. Geme, THE HAEMOPHILUS INFLUENZAE HMW1 ADHESIN IS A GLYCOPROTEIN WITH AN UNUSUAL N-LINKED CARBOHYDRATE MODIFICATION, JBC Papers in Press. Published on July 11, 2008	Click	"All digestions were acidified to 5% formic acid, and desalted peptides were prepared using a NuTip carbon tip (Glygen) according to the manufacturer's instructions."	NuTip	Carbon
Glyco-peptide	Purification / desalting	2008	Christoph Kannicht, Detlef Grunow and Lothar Lucka, Enzymatic Sequence Analysis of N -Glycans by Exoglycosidase Cleavage and Mass Spectrometry detection of Lewis X Structures, Methods in Molecular Biology, Volume 446, Post-translational Modifications of Proteins, Humana Press, p. 255-266, (2008)	Click	"TopTip carbon spin column, type P2-Carbon (Glygen Corp., Columbia, USA) for sample purification"	NuTip	Carbon
Glyco-peptide	Purification / desalting	2008	Adam W. Lucka, Bruce R. Kilgore, Rekha Patel, Bruce A. Andrien Jr. and Shirish T. Dhume, Mass Spectrometry and HPLC with Fluorescent Detection-Based Orthogonal Approaches to Characterize N -Linked Oligosaccharides of Recombinant Monoclonal Antibodies, Methods in Molecular Biology, Volume 446, Post-translational Modifi cations of Proteins, Humana Press, p. 347-36 (2008)	Click	"Hypercarb TopTip (Glygen Corp) (available in 3 binding-capacity levels)"	NuTip	Carbon
Glyco-peptide	Purification / desalting	2008	Rapha Carapito, Christine Carapito, Jean-Marc Jeltsch and Vincent Phalip,Efficient hydrolysis of hemicellulose by a Fusarium graminearum xylanase blend produced at high levels in Escherichia coli., Bioresource Technology, Article in Press, Corrected Proof doi:10.1016/j.biortech. 2008.	Click	"…Polysaccharideswere desalted using a NuTip type P20 carbon solid-phase extraction pipette tip (Glygen, USA)."	NuTip	Carbon
Glyco-protein	Enrichment	2010	Mizuho INAGAKI, Shuuichi NAKAYA, Daisuke NOHARA, Tomio YABE, Yoshihiro KANAMARU and Tohru SUZUKI, “The Multiplicity of N-Glycan Structures of Bovine Milk 18 kDa Lactophorin (Milk GlyCAM-1)”, Biosci. Biotechnol. Biochem., Vol. 74, 447-450 (2010) .	Click	"The PA-derivatized glycans were then dried with a centrifugal evaporator and dissolved in 10 цl of 0.1% v/v trifluoroacetic acid, followed by purified via Nutip Carbon (Glygen, Columbia, MD) according to the manufacturer's protocol."	NuTip	HyperCarb
Glyco-protein	Enrichment	2010	John W. Froehlich, Eric D. Dodds, Mariana Barboza, Erica L. McJimpsey, Richard R. Seipert, Jimi Francis, Hyun Joo An, Samara Freeman, J. Bruce German, Carlito B. Lebrilla Glycoprotein Expression in Human Milk during Lactation Journal of Agricultural and Food Chemistry 2010 58 (10), 6440-6448	Click	"Porous graphitized carbon cartridges were obtained from Glygen. "	NuTip	HyperCarb
Glyco-protein	Enrichment	2010	Ming Lei, et al, 'Sequential Enrichment of Sulfated Glycans by Strong Anion-Exchange Chromatography Prior to Mass Spectrometric Measurements,' J Am Soc Mass Spectrom, 2010, 21, 348-357	Click	"Sequential enrichment of sulfated glycans is attained using spin-columns packed with strong anion exchanger"	TopTip	SAX
Glyco-protein	Enrichment	2008	DS Spellman, TA Neubert - Analysis of Posttranslational Modifications, Proteomics of the Nervous System, 2008 – books.google.com			NuTip and TopTip	POROS RP2
Glyco-protein	Enrichment	2008	JT Adamson Structural Characterization of Protein Glycosylation Utilizing Fragmentation from Gas Phase Ion- , - 2008 -, ... A dissertation submitted in partial fulfillment of the requirements for the degree, of Doctor of Philosophy (Chemistry) in The University of Michigan 2008.			NuTip and TopTip	POROS RP2
Glyco-protein	Enrichment	2006	An HJ, Miyamoto S, Lancaster KS, Kirmiz C, Li B, Lam KS, Leiserowitz GS, Lebrilla CB. Related Articles, Profiling of glycans in serum for the discovery of potential biomarkers for ovarian cancer. J Proteome Res. 2006 Jul;5(7):1626-35.	Click		TopTip	Hypercarb
Glyco-protein	Enrichment	2004	N. I. Taranenko, A. K. Shukla, M. M. Shukla, V. M. Doroshenko Effects of Lectins Affinity Chromatography on Glycoproteins Enrichment Using AP-MALDI Ion Trap Mass Spectrometry. (ASMS 2004)
Glyco-protein	Purification / desalting	2010	Hiroto Hirayama, Junichi Seino, Toshihiko Kitajima, Yoshifumi Jigami and Tadashi Suzuki Free Oligosaccharides to Monitor Glycoprotein Endoplasmic Reticulum-associated Degradation in Saccharomyces cerevisiae The Journal of Biological Chemistry, 285, 12390-12404.	Click	"For desalting of PA-oligosaccharide-containing fractions, we used C18+ carbon NuTip (Hypercrb, Glygen, Columbia, MD) as described previously"	NuTip	C18+HyperCarb
Oligo-saccharide	Purification / desalting	2009	Raphaël Carapito, Christine Carapito, Jean-Marc Jeltsch and Vincent Phalip, Bioresource Technology, Volume 100, Issue 2, January 2009, Pages 845-850, Efficient hydrolysis of hemicellulose by a Fusarium graminearum xylanase blend produced at high levels in Escherichia coli	Click	"...the polysaccharides were desalted using a NuTip type P20 carbon solid-phase extraction pipette tip (Glygen, USA)."	NuTip	P20
Oligo-saccharide	Purification / desalting	2005	Lei Zhu, et.al Production of human monoclonal antibody in eggs of chimeric chickens, Nature Biotechnology 23, 1159 - 1169 (2005)	Click	"The released carbohydrates were desalted on a micro-carbon column from Glygen essentially following the vendor's protocol"	TopTip	Hypercarb
Oligo-saccharide	Purification / desalting	2009	Raphael Carapito, Christine Carapito, Jean-Marc Jeltsch, Vincent Phalip, Efficient hydrolysis of hemicellulose by a Fusarium graminearum xylanase blend produced at high levels in Escherichia coli, Bioresource Technology, Volume 100, Issue 2, January 2009, Pages 845-850.	Click	"...the polysaccharides were desalted using a NuTip type P20 carbon solid-phase extraction pipette tip (Glygen, USA)."	NuTip	P-20
Protein	Digestion	2007	Václav eovský , Jan Pohl , Zhihua Yang , Naseer Alam, Athula, B. Attygalle, Identification of three novel peptides isolated from the venom of the neotropical social wasp Polistes major major, Journal of Peptide Science Volume 13, Issue 7 (2007), Pages 445 – 450	Click	"PMM2 dissolved in ammonium bicarbonate buffer (20 µl, 50 mM, pH ∼ 7.8) was aspirated into a NuTip Trypsin (Bovine) tip (Glygen Corp., MD, USA) and kept for 5 min."	NuTip	Trypsin
Protein	Digestion	2005	Ashok K. Shukla; Mukta M Shukla; Alexis A. Oetting; Nelli I. Taranenko; Vladimir M. Doroshenko; Application of Non-Covalently Bound Trypsin on Chromatographic Material for Digestion of Proteins, ASMS 2005 TP-09, poster 144
Protein	Enrichment	2008	Kenneth M. Wannemacher, Alexandra Terskiy, Shengjie Bian, Prem N. Yadav, Hong Li, Richard D. Howells, Purification and mass spectrometric analysis of the [kappa] opioid receptor, Brain Research, Volume 1230, 16 September 2008, Pages 13-26.	Click		NuTip	C18
Protein	Enrichment	2008	Protein Hydrolysate Compositions Having Improved Sensory Characteristics and Physical Properties , United States Patent Application 20080305212.	Click
Protein	Enrichment	2008	S Hansen et al. Antibodies against insulin-like growth factor I receptor and uses thereof United States Patent Application 20080226635.	Click
Protein	Enrichment	2008	Joshua F. Alfaro, Laura A. Gillies, He G. Sun, Shujia Dai, Tianzhu Zang, Joshua J. Klaene, Byung Ju Kim, Jonathan D. Lowenson, Steven G. Clarke, Barry L. Karge and Zhaohui Sunny Zhou, Chemo-Enzymatic Detection of Protein Isoaspartate Using Protein Isoaspartate Methyltransferase and Hydrazine Trapping, Anal. Chem., 2008, 80 (10), pp 3882–3889.	Click	"Centrifuge adapters purchased from Glygen (Columbia, MD) were used to suspend columns above 1.5 mL centrifuge tubes during centrifugation. "	Centrifuge adaptors
Protein	Enrichment	2007	Emma Lundberg, Mårten Sundberg, Torbjörn Gräslund, Mathias Uhlén and Helene Andersson Svahn. A novel method for reproducible fluorescent labeling of small amounts of antibodies on solid phase. doi:10.1016/j.jim.2007.01.023, Journal of Immunological Methods Volume 322, Issues 1-2, 30 April 2007, Pages 40-49	Click	"Micropipette tips (TopTip 10–200 μl) filled with protein A affinity medium were obtained from Glygen Corp."	TopTip	Protein A
Protein	Enrichment	2007	Sandra E. Wiley, Mark L. Paddock, Edward C. Abresch, Larry Gross, Peter van der Geer, Rachel Nechushtai, Anne N. Murphy, Patricia A. Jennings and Jack E. Dixon. The Outer Mitochondrial Membrane Protein mitoNEET Contains a Novel Redox-active 2Fe-2S Cluster, J. Biol. Chem., Vol. 282, Issue 33, 23745-23749, August 17, 2007	Click	"Untagged mitoNEET 33–108 (0.25 μm) was desalted by applying 20 μl of sample to a solid phase extraction pipette tip (TopTip, Glygen Corp.) containing 30 μl of Poros20 reverse phase C18-polymer resin"	TopTip	POROS RP2
Protein	Enrichment	2003	Andrew J. Alpert and Ashok K. Shukla. Precipitation of Large Proteins from Serum with Organic Solvemts. ASMS 2003
Protein	Purification	2010	Wim Fremout, Maarten Dhaenens, Steven Saverwyns, Jana Sanyova, Peter Vandenabeele, Dieter Deforce, Luc Moens, Tryptic peptide analysis of protein binders in works of art by liquid chromatography-tandem mass spectrometry, Analytica Chimica Acta, Volume 658, Issue 2, 25 January 2010, Pages 156-162.	Click		TopTip	HILIC
Protein	Purification	2009	On-Chip Solid-Phase Extraction Preconcentration/Focusing Substrates Coupled to Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization Ion Trap Mass Spectrometry for High Sensitivity Biomolecule Analysis. Arti Navare, Marcela Nouzova, Fernando G. Noriega, Salvador Hernández-Martínez, Christoph Menzel, and Facundo M. Fernández. Rapid Commun Mass Spectrom. 2009 February; 23(4): 477–486.	Click		NuTip	C18
Protein	Purification	2009	David R.H. Evans, Jonathan K. Romero, Matthew Westoby, Chapter 9 Concentration of Proteins and Removal of Solutes, In: Richard R. Burgess and Murray P. Deutscher, Editor(s), Methods in Enzymology, Academic Press, 2009, Volume 463, Pages 97-120.	Click	"Examples of micropipette-based products are … NuTips (Glygen, Columbia, MD) in which the interior walls of the tip are coated with the binding."	NuTip
Protein	Purification / desalting	2009	Kristin R. Wildsmith, Bomie Han and Randall J. Bateman, Analytical Biochemistry Volume 395, Issue 1, 1 December 2009, Pages 116-118, Method for the simultaneous quantitation of apolipoprotein E isoforms using tandem mass spectrometry.	Click	"Before analysis, samples were desalted using Carbon Nu-tips following the manufacturer's instructions (Glygen, Columbia, MD)."	NuTip	Carbon
Protein	Purification / desalting	2009	Mahmoud Toorchi, Kiyoshi Yukawa, Mohammad-Zaman Nouri, Setsuko Komatsu, Proteomics approach for identifying osmotic-stress-related proteins in soybean roots, Peptides, Volume 30, Issue 12, December 2009, Pages 2108-2117.	Click	"..desalted with NuTip C-18 pipette tips (Glygen, Columbia, MD)"	NuTip	C18
Protein	Purification / desalting	2009	Setsuko Komatsu, Takuya Wada, Yann Abal, Mohammad-Zaman Nouri, Yohei Nanjo, Norikazu Nakayama, Satoshi Shimamura, Ryo Yamamoto, Takuji Nakamura and Kiyoshi Furukawa , Analysis of Plasma Membrane Proteome in Soybean and Application to Flooding Stress Response, J. Proteome Res., 2009, 8 (10), pp 4487–4499.	Click	"...desalted with NuTip C-18 pipet tips (Glygen, Columbia, MD)"	NuTip	C18
Protein	Purification / desalting	2009	KR Wildsmith, B Han, RJ Bateman. Method for the simultaneous quantitation of apolipoprotein E isoforms using tandem mass spectrometry. Analytical Biochemistry, Volume 395, Issue 1, 1 December 2009, Pages 116-118,	Click	"…Samples were desaltedusing Carbon Nu-tips following the manufacturer's instructions (Glygen, Columbia, MD)"	NuTip	HyperCarb
Small molecule	Enrichment	2009	Siegel Marshall M: "GPC Spin Column HPLC–ESI-MS Methods for Screening Drugs Noncovalently Bound to Proteins," Ligand-Macromolecular Interactions in Drug Discovery: Methods and Protocols, 2009.	Click		TopTip
Small molecule	Enrichment	2007	MM Siegel - Mass spectrometry in medicinal chemistry, 2007 - books.google.com Drug Screening Using Gel Permeation Chromatography Spin Columns Coupled with ESI
		2009	Sebastian Leidel, Patrick G. A. Pedrioli, Tamara Bucher, Renée Brost, Michael Costanzo, Alexander Schmidt, Ruedi Aebersold, Charles Boone, Kay Hofmann& Matthias Peter, Ubiquitin-related modifier Urm1 acts as a sulphur carrier in thiolation of eukaryotic transfer RNA, Nature 458, 228-232 (12 March 2009)	Click		NuTip	TiO2
		2009	Serum biomarker profiling by solid-phase extraction with particle-embedded micro tips and matrix-assisted laser desorption/ionization mass spectrometry. Arti Navare, Manshui Zhou, John McDonald, Fernando G. Noriega, M. Cameron Sullards, and Facundo M. Fernandez. Rapid Commun Mass Spectrom. Author manuscript; available in PMC 2009 April 6.	Click
		2009	Stable Oxygen Isotope Labeling of Pre-existing Phosphoryl Groups on Phosphomolecules for Modification-Specific Mass Spectrometry United States Patent Application 20090142849.
		2008	Brian Roberts, Jan Pohl, Jennifer L. Gooch, A fluorimetric method for determination of calcineurin activity, Cell Calcium, Volume 43, Issue 5, May 2008, Pages 515-519.	Click	"Titanium-oxide (TiO 4 )-coated plates were obtained from Glygen (Colombus, MA)"	Coated plates	TiO2
		2007	Koji Ikegam, Robb L. Heier, Midori Taruishi, , Hiroshi Takagi, Masahiro Mukai, Shuichi Shimma, Shu Taira, Ken Hatanaka , Nobuhiro Morone, Ikuko Yao, Patrick K. Campbell, Shigeki Yuasa, Carsten Janke, Grant R. MacGregor , and Mitsutoshi Setou, Loss of -tubulin polyglutamylation in ROSA22 mice is associated with abnormal targeting of KIF1A and modulated synaptic function, PNAS \| February 27, 2007 \| vol. 104 \| no. 9 \| 3213-3218	Click	"An anion exchange tip (Nutip; Glygen, Columbia, MD) was used to desalt the sample."	NuTip	Anion Exchange
		2007	Gerrit J. Schut, Stephanie L. Bridger, and Michael W. W. Adams, Insights into the Metabolism of Elemental Sulfur by the Hyperthermophilic Archaeon Pyrococcus furiosus: Characterization of a Coenzyme A- Dependent NAD(P)H Sulfur Oxidoreductase, Journal of Bacteriology, June 2007, p. 4431-4441, Vol. 189, No. 12	Click	"Peptides were purified using NuTip C 18 tips (Glygen Corp., Columbia, MD) and spotted (1 µl, containing -cyano-4-hydroxycinnamic acid) directly on a matrix-assisted laser desorption ionization plate."	NuTip	C18
		2007	Angie R. Purvis, Julia Gross, Luke T. Dang, Ren-Huai Huang, Milan Kapadia, R. Reid Townsend, and J. Evan Sadler: Two Cys residues essential for von Willebrand factor multimer assembly in the Golgi, PNAS October 2, 2007 vol. 104 no. 40 15647-15652.	Click	"Reactions were desalted by adsorption on C18 micropipette tips (Glygen Corp., Columbia, MD) and elution with 60% acetonitrile/0.1% formic acid."	NuTip	C18
		2007	Ashok K. Shukla; Mukta Shukla: Reversed Gel Filtration for the Sample Preparation. (POSTER ASMS 2007)
		2006	Mukta M. Shukla, Ashok K. Shukla, Nelli I. Taranenko, Vladimir M. Doroshenko, Sample Concentrator for AP-MALDI Plate. ASMS 2006.
		2005	Christopher J. Toher , Adam W. Perala , Ashok K. Shukla , Gary A. Valaskovic Sample Purification for Static Nanospray MS Using Wall-Coated Pipette Trap'nTips™ , \| (ASMS 2005)
		2005	MM Shukla, AK Shukla, G Barka, N Manohar, NI Taranenko Use of Chromatographic Hollow Fiber for Purification and Separation of Peptides for AP-MALDI, ABRF 2005; P37-S.	Click
		2004	Tassilo Muskat, Ph.D. Thesis 2004. Matrixabhangiges Ablationsverhalten von Neutralen und Oberflahencharakteristika bei der Matrix Assisted Laser Desorption and Ionisation.	Click		NuTip	Carbon
		2004	Mukta M. Shukla , Ashok K. Shukla, Vladimir M. Doroshenko, Nelli I. Taranenko Nanoliter Solid Phase Extraction (SPE) Using Chromatographic Hollow Fibers for Sample Preparation for Mass Spectrometry (ASMS 2004)
		2003	Ashok K. Shukla, Mukta M. Shukla, Eric D. Stover, and Andreas F. Huhmer. Application of Mixed-Mode Solid-PhaseExtraction (MMSPE) for Proteomics Sample Preparation. AMERICAN BIOTECHNOLOGY LABORATORY / OCTOBER 2003	Click		NuTip	HyperCarb + C18
		2003	Mukta M. Shukla, Ashok K. Shukla, Eric D. Stover, and Andreas F. Huhmer. Chromatographic Hollow Fiber. ASMS 2003

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